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The map label for this gene is lexA

Identifier: 160877411

GI number: 160877411

Start: 5092489

End: 5093109

Strand: Direct

Name: lexA

Synonym: Sbal195_4309

Alternate gene names: 160877411

Gene position: 5092489-5093109 (Clockwise)

Preceding gene: 160877407

Following gene: 160877412

Centisome position: 95.24

GC content: 46.86

Gene sequence:

>621_bases
ATGAGACCTTTGACGCCACGCCAAGCTGAAATTTTAGAGCTAATTAAACGAAATATTGCCGATACTGGCATGCCACCCAC
TCGGGCAGAAATCGCGACCCGTTTAGGTTTTAAGAGCGCCAATGCGGCTGAAGAGCATCTTAAAGCGTTGGCGAAAAAGG
GCTGCATTGAGATCATGCCAGGCACATCGCGGGGCATTCGTCTGACTGCGGAAGTTGAAGAAGTAACGGAAACAGGTTTA
CCGCTTATCGGTCAAGTAGCCGCTGGCGAACCTATTCTCGCCCAAGAACATGTTGAACAGTACTACCAAGTCGACCCAAG
CATGTTCCATCCCGCCGCCGATTTTTTACTGCGGGTTAAAGGCGACAGCATGAAGAATATTGGCATTCTCGAAGGAGATT
TGCTGGCAGTTCATAAAGTACAACAAGCTAGAAATGGTCAAGTGGTTGTCGCTAGAGTCGACGACGATGTCACTGTTAAA
CGTTTCGAAAAAAAAGGTAATGTGGTCTATCTACACGCCGAAAATGAAGACTATTCCCCGATTAAAGTGGATTTAGGCTA
TCAAAGTTTAACAATTGAAGGACTCGCCGTCGGTGTGATCCGTAATGGAGATTGGCTATGA

Upstream 100 bases:

>100_bases
TCGCTAATTCTCTCCAACATAGCTAAAAAGTGGTTAATACTTGCGCTGCAAATAATACTGTATATACTAACAGTAACTGT
ATAGAAAAACAGGAAACACC

Downstream 100 bases:

>100_bases
ACAAACTACTTGGCAATGCACCCCGCCACCCGGGCTTGTGGCTCGATGAAGTCCGTGATGCCGACTATGACTGGCACCAT
GCACCTATCGCCTGCATGCC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MRPLTPRQAEILELIKRNIADTGMPPTRAEIATRLGFKSANAAEEHLKALAKKGCIEIMPGTSRGIRLTAEVEEVTETGL
PLIGQVAAGEPILAQEHVEQYYQVDPSMFHPAADFLLRVKGDSMKNIGILEGDLLAVHKVQQARNGQVVVARVDDDVTVK
RFEKKGNVVYLHAENEDYSPIKVDLGYQSLTIEGLAVGVIRNGDWL

Sequences:

>Translated_206_residues
MRPLTPRQAEILELIKRNIADTGMPPTRAEIATRLGFKSANAAEEHLKALAKKGCIEIMPGTSRGIRLTAEVEEVTETGL
PLIGQVAAGEPILAQEHVEQYYQVDPSMFHPAADFLLRVKGDSMKNIGILEGDLLAVHKVQQARNGQVVVARVDDDVTVK
RFEKKGNVVYLHAENEDYSPIKVDLGYQSLTIEGLAVGVIRNGDWL
>Mature_206_residues
MRPLTPRQAEILELIKRNIADTGMPPTRAEIATRLGFKSANAAEEHLKALAKKGCIEIMPGTSRGIRLTAEVEEVTETGL
PLIGQVAAGEPILAQEHVEQYYQVDPSMFHPAADFLLRVKGDSMKNIGILEGDLLAVHKVQQARNGQVVVARVDDDVTVK
RFEKKGNVVYLHAENEDYSPIKVDLGYQSLTIEGLAVGVIRNGDWL

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=206, Percent_Identity=69.9029126213592, Blast_Score=292, Evalue=1e-80,
Organism=Escherichia coli, GI1787431, Length=134, Percent_Identity=32.8358208955224, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_SHEB2 (B8ECL9)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002360004.1
- ProteinModelPortal:   B8ECL9
- SMR:   B8ECL9
- MEROPS:   S24.001
- GeneID:   7089731
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_4110
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22635; Mature: 22635

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: ACT_SITE 123-123 ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLTPRQAEILELIKRNIADTGMPPTRAEIATRLGFKSANAAEEHLKALAKKGCIEIMP
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECC
GTSRGIRLTAEVEEVTETGLPLIGQVAAGEPILAQEHVEQYYQVDPSMFHPAADFLLRVK
CCCCCEEEEEEHHHHHHCCCCEEEHHCCCCCCHHHHHHHHHHHCCCHHHCCHHHEEEEEC
GDSMKNIGILEGDLLAVHKVQQARNGQVVVARVDDDVTVKRFEKKGNVVYLHAENEDYSP
CCCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCC
IKVDLGYQSLTIEGLAVGVIRNGDWL
EEEECCCCEEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MRPLTPRQAEILELIKRNIADTGMPPTRAEIATRLGFKSANAAEEHLKALAKKGCIEIMP
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECC
GTSRGIRLTAEVEEVTETGLPLIGQVAAGEPILAQEHVEQYYQVDPSMFHPAADFLLRVK
CCCCCEEEEEEHHHHHHCCCCEEEHHCCCCCCHHHHHHHHHHHCCCHHHCCHHHEEEEEC
GDSMKNIGILEGDLLAVHKVQQARNGQVVVARVDDDVTVKRFEKKGNVVYLHAENEDYSP
CCCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCC
IKVDLGYQSLTIEGLAVGVIRNGDWL
EEEECCCCEEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA