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The map label for this gene is rna [H]

Identifier: 160877407

GI number: 160877407

Start: 5086476

End: 5087645

Strand: Direct

Name: rna [H]

Synonym: Sbal195_4305

Alternate gene names: 160877407

Gene position: 5086476-5087645 (Clockwise)

Preceding gene: 160877406

Following gene: 160877411

Centisome position: 95.12

GC content: 50.26

Gene sequence:

>1170_bases
ATGACCAAGCCATTTTGTTTGACCATCCGCCGGATATGGCCGCAAGCACTTTCCCTTAAAAGCCTATCGCGTACGTTACT
TGCGGCGTTCGGTTTATGCGCTGCGCTAATCATTTCAGCAGCACCTGCCTTTGCCGCGCCCGCAACCGGTGAGTTCGTTG
CCGATAAACGCTGTGAGCTATTTCAATCGAAAAATAAGCAGACGAATCCAGATCAATGGCAGAGCAATATTGGTGAGCGC
TATCCGGTACTCGAAATCCTCGGCAACAGCGCCAAACCTGATTGGCTGCGAGTGCGCACCAACGCAGTGAAGTCTCCCAT
GCGGTGGATCAGTGGCGCTTGTGGTCAGTATCTTTCCAATGTTGCAGGCAGTAATGTATCGACACCTGCAACAGCGGTAG
AACCCGCGCAAACCACTACAGCATCGGATATCAAAGCCAAAAGCCGAGACGGCCTTAAAGAGCATGGCACACCAGAGAAA
CGCCAAGGAGATCAATGCCGAATCGGCGGTGAATTTGACGCCAACGTGCTGGCATTAAGCTGGCAAAGCACCTTCTGCGA
ACTCTATGGCCGCCGCAAAGCCGAATGCCGCGCCCTCAGCCAAACGCCAGAATCGAGCCAGTGGCAACACTTCAGCCTGC
ACGGGCTGTGGCCGAATAAACAACAATGCGGCACACGTTATGGCTATTGCAGCACAGTCAAACTGCAGCCGAATGATTTC
TGTACTTATCCTGAGTTTGAACTGAATCCATCGGTGCGCAAAAATTTAGAAGAAGTCATGCCAAGCGCCCAATATGGCAC
CTGTTTAGAACGCCACGAATGGTGGAAACACGGCACTTGCCGTAGCCAAGACCCTAACGAGTACTTTTTATTGGCAACCC
AGCTGACCCAAGCGGTCAATGCCTCGGCATGGGTACAAAAATTTATTCATAACAACATAGGTAAGAGTGTCAGTAAAAGT
GAGTTAAACCAAAGTTTTGATAGCAGTTTCGGTCAAGGTGCTCACGCTAAAATGAGCCTAGAATGTGGCAAGGGATTACT
GAGTGAAATCCGCATTAACTTGCCCGAGGTCATTAAAGCCTCAGACTCCTTGCCTAGCCTACTCGCCAAAGCTAACAAGG
CAGGCAGAGGCTCCTGCCCTGAGACAATCATGATTGATAATGCTAATTAA

Upstream 100 bases:

>100_bases
GAGTAAAATAAGCCTCTTAGTTTATAACGCTTATGTGTACCATTAACTTAGCGCACCACAGGATTGACCCGACTTACTGC
AGCAGAAAGGATTCCATCAC

Downstream 100 bases:

>100_bases
ACAAATGGTTAGACGAATAAAAATGGCGACTAAGTGTCGCCGTTTTTAATCTTAAATGTGACTCGTCCTAAATATAGCTG
TCACATCTGATTAAGCTTGA

Product: ribonuclease T2

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTKPFCLTIRRIWPQALSLKSLSRTLLAAFGLCAALIISAAPAFAAPATGEFVADKRCELFQSKNKQTNPDQWQSNIGER
YPVLEILGNSAKPDWLRVRTNAVKSPMRWISGACGQYLSNVAGSNVSTPATAVEPAQTTTASDIKAKSRDGLKEHGTPEK
RQGDQCRIGGEFDANVLALSWQSTFCELYGRRKAECRALSQTPESSQWQHFSLHGLWPNKQQCGTRYGYCSTVKLQPNDF
CTYPEFELNPSVRKNLEEVMPSAQYGTCLERHEWWKHGTCRSQDPNEYFLLATQLTQAVNASAWVQKFIHNNIGKSVSKS
ELNQSFDSSFGQGAHAKMSLECGKGLLSEIRINLPEVIKASDSLPSLLAKANKAGRGSCPETIMIDNAN

Sequences:

>Translated_389_residues
MTKPFCLTIRRIWPQALSLKSLSRTLLAAFGLCAALIISAAPAFAAPATGEFVADKRCELFQSKNKQTNPDQWQSNIGER
YPVLEILGNSAKPDWLRVRTNAVKSPMRWISGACGQYLSNVAGSNVSTPATAVEPAQTTTASDIKAKSRDGLKEHGTPEK
RQGDQCRIGGEFDANVLALSWQSTFCELYGRRKAECRALSQTPESSQWQHFSLHGLWPNKQQCGTRYGYCSTVKLQPNDF
CTYPEFELNPSVRKNLEEVMPSAQYGTCLERHEWWKHGTCRSQDPNEYFLLATQLTQAVNASAWVQKFIHNNIGKSVSKS
ELNQSFDSSFGQGAHAKMSLECGKGLLSEIRINLPEVIKASDSLPSLLAKANKAGRGSCPETIMIDNAN
>Mature_388_residues
TKPFCLTIRRIWPQALSLKSLSRTLLAAFGLCAALIISAAPAFAAPATGEFVADKRCELFQSKNKQTNPDQWQSNIGERY
PVLEILGNSAKPDWLRVRTNAVKSPMRWISGACGQYLSNVAGSNVSTPATAVEPAQTTTASDIKAKSRDGLKEHGTPEKR
QGDQCRIGGEFDANVLALSWQSTFCELYGRRKAECRALSQTPESSQWQHFSLHGLWPNKQQCGTRYGYCSTVKLQPNDFC
TYPEFELNPSVRKNLEEVMPSAQYGTCLERHEWWKHGTCRSQDPNEYFLLATQLTQAVNASAWVQKFIHNNIGKSVSKSE
LNQSFDSSFGQGAHAKMSLECGKGLLSEIRINLPEVIKASDSLPSLLAKANKAGRGSCPETIMIDNAN

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=247, Percent_Identity=32.7935222672065, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 43000; Mature: 42869

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPFCLTIRRIWPQALSLKSLSRTLLAAFGLCAALIISAAPAFAAPATGEFVADKRCEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FQSKNKQTNPDQWQSNIGERYPVLEILGNSAKPDWLRVRTNAVKSPMRWISGACGQYLSN
HHHCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
VAGSNVSTPATAVEPAQTTTASDIKAKSRDGLKEHGTPEKRQGDQCRIGGEFDANVLALS
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCCCCEEEEE
WQSTFCELYGRRKAECRALSQTPESSQWQHFSLHGLWPNKQQCGTRYGYCSTVKLQPNDF
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECCCCC
CTYPEFELNPSVRKNLEEVMPSAQYGTCLERHEWWKHGTCRSQDPNEYFLLATQLTQAVN
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHH
ASAWVQKFIHNNIGKSVSKSELNQSFDSSFGQGAHAKMSLECGKGLLSEIRINLPEVIKA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHC
SDSLPSLLAKANKAGRGSCPETIMIDNAN
CCHHHHHHHHHCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
TKPFCLTIRRIWPQALSLKSLSRTLLAAFGLCAALIISAAPAFAAPATGEFVADKRCEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FQSKNKQTNPDQWQSNIGERYPVLEILGNSAKPDWLRVRTNAVKSPMRWISGACGQYLSN
HHHCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
VAGSNVSTPATAVEPAQTTTASDIKAKSRDGLKEHGTPEKRQGDQCRIGGEFDANVLALS
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCCCCEEEEE
WQSTFCELYGRRKAECRALSQTPESSQWQHFSLHGLWPNKQQCGTRYGYCSTVKLQPNDF
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECCCCC
CTYPEFELNPSVRKNLEEVMPSAQYGTCLERHEWWKHGTCRSQDPNEYFLLATQLTQAVN
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHH
ASAWVQKFIHNNIGKSVSKSELNQSFDSSFGQGAHAKMSLECGKGLLSEIRINLPEVIKA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHC
SDSLPSLLAKANKAGRGSCPETIMIDNAN
CCHHHHHHHHHCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]