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The map label for this gene is prpB [H]

Identifier: 160877247

GI number: 160877247

Start: 4907430

End: 4908284

Strand: Direct

Name: prpB [H]

Synonym: Sbal195_4144

Alternate gene names: 160877247

Gene position: 4907430-4908284 (Clockwise)

Preceding gene: 160877246

Following gene: 160877248

Centisome position: 91.77

GC content: 48.77

Gene sequence:

>855_bases
ATGAAAAAAACAACGGCATTACGACAAATGCTCAGTGGTAGCGATATTCACGTCGCCCCCGGTGTTTACGATGGTATGAC
CGCACGTTTAGCCGAACAAACGGGTTTTAAGCTGATTTATGCCAGTGGCGGCGCGATTGCGAGAAGTTGTGGTTTCCCCG
ATATAGGCATGCTGAGCTTTAGCGAAGTGCTGCATCGACTCGAGCAAATGGTTGAAGTGACCCAAGTCCCCATCATAGCG
GATGCGGATACTGGGTTTGGTAATGCCATCAACGTGAGTCGCACAGTAAAAGCCTTTGAGCGAGCCGGTGTCGCAGCATT
GCACTTGGAGGATCAAACCTTTCCAAAGCGCTGCGGCCACCTCAATGATAAATCTTTGGTGAGTACCCAAGAAATGGTGC
ACAAGATCCGTGTCGCTAAGGACAGCCAAACTGACCCAGATTTTACCTTAATTGCTCGCACTGATGCGATTGCCGTTGAA
GGATTCGATGCTGCAATGGAACGCTCACATGCGTATTTAGCAGCGGGTGCCGACGTGATTTTTGTCGAAGCGCCCGAAAC
CATTGAGCAAATTGAGTTGATCGCTAAGCATATCAAGCAGCCTAAGCTCATCAATATGTTCCACAGTGGTAAAACACCGC
TGGTGTCGAAGGACAGACTGCAAGCATTGGGATACAAGTTCATCATTATTCCCTCAGACTTACAGCGAGCGACTATACAT
GCTTGTCAGCACACCTTACGCACCATTCTTGAGCAAGGTGACAGTGGCAGTATTGCCGAGCAGATGGTGTCGTTTGCAGA
GCGAGAACGGATCATAAATACACAAGCATATTTGGATCTTGACCTCGGCTATTGA

Upstream 100 bases:

>100_bases
GTGCTAATGGAAGGTTTTGTGCGAGTACCGAGCGAGTGTTTCTGATCGTTTATCTGGTTTTAGATTCAATCGCTAAAGGC
GCGCAAGGAAGAGGATTCAA

Downstream 100 bases:

>100_bases
CGTAACTGAGTCAATGCAAGTTTAACCCTGCTATCTAATCGCTAAAACAGTTCAACCAAAGACCTTGCTTCTCCCCCTTC
CGGCAAGGTCTTTTTCTATT

Product: isocitrate lyase family protein

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQTGFKLIYASGGAIARSCGFPDIGMLSFSEVLHRLEQMVEVTQVPIIA
DADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGHLNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVE
GFDAAMERSHAYLAAGADVIFVEAPETIEQIELIAKHIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTQAYLDLDLGY

Sequences:

>Translated_284_residues
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQTGFKLIYASGGAIARSCGFPDIGMLSFSEVLHRLEQMVEVTQVPIIA
DADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGHLNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVE
GFDAAMERSHAYLAAGADVIFVEAPETIEQIELIAKHIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTQAYLDLDLGY
>Mature_284_residues
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQTGFKLIYASGGAIARSCGFPDIGMLSFSEVLHRLEQMVEVTQVPIIA
DADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGHLNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVE
GFDAAMERSHAYLAAGADVIFVEAPETIEQIELIAKHIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTQAYLDLDLGY

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=274, Percent_Identity=39.4160583941606, Blast_Score=200, Evalue=8e-53,
Organism=Escherichia coli, GI1790445, Length=203, Percent_Identity=32.512315270936, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=213, Percent_Identity=30.9859154929577, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982926, Length=213, Percent_Identity=30.9859154929577, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31171; Mature: 31171

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQTGFKLIYASGGAIARSCGFPDIGMLSF
CCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCHHHH
SEVLHRLEQMVEVTQVPIIADADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGH
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCC
LNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVEGFDAAMERSHAYLAAGADVI
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCHHHHHHHCCEEEEECCCEE
FVEAPETIEQIELIAKHIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
EEECCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCHHHHHHCCEEEEEECCCHHHHHHH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTQAYLDLDLGY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQTGFKLIYASGGAIARSCGFPDIGMLSF
CCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCHHHH
SEVLHRLEQMVEVTQVPIIADADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGH
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCC
LNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVEGFDAAMERSHAYLAAGADVI
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCHHHHHHHCCEEEEECCCEE
FVEAPETIEQIELIAKHIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
EEECCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCHHHHHHCCEEEEEECCCHHHHHHH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTQAYLDLDLGY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]