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The map label for this gene is mii [H]

Identifier: 160877246

GI number: 160877246

Start: 4906151

End: 4907374

Strand: Direct

Name: mii [H]

Synonym: Sbal195_4143

Alternate gene names: 160877246

Gene position: 4906151-4907374 (Clockwise)

Preceding gene: 160877245

Following gene: 160877247

Centisome position: 91.75

GC content: 53.43

Gene sequence:

>1224_bases
ATGAATGCTTCAACAACCCACGCGCCACAAATCAAAGTTGCTGCGACCTATATGCGCGGCGGCACCAGTAAAGGCGTGTT
TTTCCGTCTGCAGGATTTACCTAAAGCCGCACAAGTTGCTGGCCCTGCCCGTGATGCGCTACTGCTGCGAGTGATTGGCA
GCCCAGACGCTTACGGCAAACAAATCGATGGCATGGGCGGCGCCACCTCAAGCACCAGTAAAACCGTGATCCTCTCCCCT
AGCACCCAAACCGATCACGATGTCGATTACCTGTTCGGCCAAGTGGCCATCGATAAACCCTTTGTCGATTGGAGTGGTAA
CTGCGGCAACTTAACCGCCGCCGTCGGCGCCTTTGCCATCAGCAATGGCTTAATTGATACGGCCCGCATTCCACAAAATG
GCATCTGCACTGTGCGTATTTGGCAAGCCAACATAGGCAAAACCATTATCGCCCATGTGCCAATCACTAAAGGTGAAGTG
CAAGAAACAGGGGATTTTGAGCTCGATGGTGTGACCTTTCCCGCCGCCGAAGTGCAAATCGAATTTATGAATCCGGCTGC
TGACGAAGATGCCCATTCTAACGGAGGGGGTAGCAGCATGTTCCCGACGGGCAATGTTGTTGATGTGCTAGAAGTGCCGG
GTATTGGCAGCTTTAAGGCCACTATGATTAACGCTGGCATTCCCACCATTTTTATCAATGCTGAGGACTTAGGCTACACA
GGCACTGAGCTGCAGGATGATATTAACAGCGACACGGCGGCACTGGCTAAATTTGAAACAATTCGCGCCTATGGCGCTGT
GCGTATGGGCTTGATTTCGCATATAGATGAAGCGATTACGCGCCAACATACGCCCAAGGTCGCCTTTGTGGCTAAACCCA
AAGCCTATGTGTCATCGAGCGGCAAGCCCGTCAATACCGATGATATCGACTTGCTCGTGCGCGCGCTGTCGATGGGTAAA
CTGCACCACGCCATGATGGGCACAGCTGCAGTGGCAATTGGCACAGCAGCAGCGATCCCCGGCACCTTAGTTAACTTAGC
CGCTAGCGGCGACACTAAGGACAAAATAGATGCGGTGCGTTTTGGTCATCCCTCTGGCACGCTGCGTGTTGGGGCTGAGG
CGTCAATGGTTGACGGCGAATGGACAGTCGTTAAAGCCATTATGAGCCGCAGCGCCCGCGTGCTAATGGAAGGTTTTGTG
CGAGTACCGAGCGAGTGTTTCTGA

Upstream 100 bases:

>100_bases
CAGCTAAAGATAAGCTAGCTAAACCGAGCGCCAATAACTTCCCCAATTAAGTTACTGACGCTCATTTGGATTCCATTGGG
AGTCCTTTTGGAGTCAATCA

Downstream 100 bases:

>100_bases
TCGTTTATCTGGTTTTAGATTCAATCGCTAAAGGCGCGCAAGGAAGAGGATTCAAATGAAAAAAACAACGGCATTACGAC
AAATGCTCAGTGGTAGCGAT

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MNASTTHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDAYGKQIDGMGGATSSTSKTVILSP
STQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAISNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEV
QETGDFELDGVTFPAAEVQIEFMNPAADEDAHSNGGGSSMFPTGNVVDVLEVPGIGSFKATMINAGIPTIFINAEDLGYT
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSSGKPVNTDDIDLLVRALSMGK
LHHAMMGTAAVAIGTAAAIPGTLVNLAASGDTKDKIDAVRFGHPSGTLRVGAEASMVDGEWTVVKAIMSRSARVLMEGFV
RVPSECF

Sequences:

>Translated_407_residues
MNASTTHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDAYGKQIDGMGGATSSTSKTVILSP
STQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAISNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEV
QETGDFELDGVTFPAAEVQIEFMNPAADEDAHSNGGGSSMFPTGNVVDVLEVPGIGSFKATMINAGIPTIFINAEDLGYT
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSSGKPVNTDDIDLLVRALSMGK
LHHAMMGTAAVAIGTAAAIPGTLVNLAASGDTKDKIDAVRFGHPSGTLRVGAEASMVDGEWTVVKAIMSRSARVLMEGFV
RVPSECF
>Mature_407_residues
MNASTTHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDAYGKQIDGMGGATSSTSKTVILSP
STQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAISNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEV
QETGDFELDGVTFPAAEVQIEFMNPAADEDAHSNGGGSSMFPTGNVVDVLEVPGIGSFKATMINAGIPTIFINAEDLGYT
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSSGKPVNTDDIDLLVRALSMGK
LHHAMMGTAAVAIGTAAAIPGTLVNLAASGDTKDKIDAVRFGHPSGTLRVGAEASMVDGEWTVVKAIMSRSARVLMEGFV
RVPSECF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=397, Percent_Identity=33.2493702770781, Blast_Score=168, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42666; Mature: 42666

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASTTHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDAYGK
CCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEEECCCCHHCC
QIDGMGGATSSTSKTVILSPSTQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAI
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHH
SNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEVQETGDFELDGVTFPAAEVQI
HCCCEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEECCEECCCEEEEE
EFMNPAADEDAHSNGGGSSMFPTGNVVDVLEVPGIGSFKATMINAGIPTIFINAEDLGYT
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHCCCC
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSS
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCC
GKPVNTDDIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAASGDTKDKIDAVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCEEE
FGHPSGTLRVGAEASMVDGEWTVVKAIMSRSARVLMEGFVRVPSECF
ECCCCCCEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNASTTHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDAYGK
CCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEEECCCCHHCC
QIDGMGGATSSTSKTVILSPSTQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAI
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHH
SNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEVQETGDFELDGVTFPAAEVQI
HCCCEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEECCEECCCEEEEE
EFMNPAADEDAHSNGGGSSMFPTGNVVDVLEVPGIGSFKATMINAGIPTIFINAEDLGYT
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHCCCC
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSS
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCC
GKPVNTDDIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAASGDTKDKIDAVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCEEE
FGHPSGTLRVGAEASMVDGEWTVVKAIMSRSARVLMEGFVRVPSECF
ECCCCCCEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA