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The map label for this gene is rsuA [H]

Identifier: 160877136

GI number: 160877136

Start: 4743274

End: 4743975

Strand: Direct

Name: rsuA [H]

Synonym: Sbal195_4032

Alternate gene names: 160877136

Gene position: 4743274-4743975 (Clockwise)

Preceding gene: 160877135

Following gene: 160877137

Centisome position: 88.7

GC content: 50.57

Gene sequence:

>702_bases
ATGCGCCTCGATAAATTCCTCTGTAAAAGCACAGATCTTGATCGTGATGCAGCAAGTGTCGCCATTATTAACGGCTCAGT
CACAGTCAATGGCACTATCGCGACAGACTGCGCGATGCAAGTGCATGAAAATAATGCTGTCTGCCTGAATGGAAAGCCGC
TAGCGCCACGTCCATCCCGCTATTTCATGCTGCACAAACCCGCCGATACCTTGTGTTCGAATGTAGATGGCGCCTACCCT
TCGCTATTTCATCTACTCGATATCGAGCACGCATTTGACCTGCACATCGTTGGTCGCTTAGATGCCGACACCACAGGCTT
AGTGCTACTCACAGACGATGGCCGCTGGTCGTTTAATATTATCTCGCCTCAATATCAGTGCGAAAAAGTCTATCGTGTCC
AACTGCGAGATCCACTCGACAGCGAAGCAACTGCAGCCATAGCTACAACGTTTGCTGCTGGGCTGCAATTACAGGGCGAA
ACGGCCCTCACTCTGCCAGCAACACTGCAGCAAGTGGATGAAAAAACCGTGCTACTCACACTCACCGAAGGCAAGTTTCA
TCAAGTCAAAAGGATGTTCGCCGCCGTGGGCAATAAAGTGATTGCCCTGCACAGAGCGCAAATTGGCGCAGTGGTCTTAG
ATGTGGACATTGGAGAATGGCGCGAGTTAACCTTAGCTGAGATAGACTCCTTCACGGCCTAA

Upstream 100 bases:

>100_bases
TTTTAACATTTATACAGAGCAGTTGCTTATCCTCCCTGAGTCGCGATAATACCGCTTACTCGAACACCCGTGCTCACTGC
TCTGCCTGAGATCTTTCACT

Downstream 100 bases:

>100_bases
GCTTTTATAGCAGAACAATTTACAGCACTCATTTCAGTGTTGTTCATTCAATCATTCCTACAAAGTCATACCTACAAACA
GCCTTGAGCTGGGTACTGAA

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MRLDKFLCKSTDLDRDAASVAIINGSVTVNGTIATDCAMQVHENNAVCLNGKPLAPRPSRYFMLHKPADTLCSNVDGAYP
SLFHLLDIEHAFDLHIVGRLDADTTGLVLLTDDGRWSFNIISPQYQCEKVYRVQLRDPLDSEATAAIATTFAAGLQLQGE
TALTLPATLQQVDEKTVLLTLTEGKFHQVKRMFAAVGNKVIALHRAQIGAVVLDVDIGEWRELTLAEIDSFTA

Sequences:

>Translated_233_residues
MRLDKFLCKSTDLDRDAASVAIINGSVTVNGTIATDCAMQVHENNAVCLNGKPLAPRPSRYFMLHKPADTLCSNVDGAYP
SLFHLLDIEHAFDLHIVGRLDADTTGLVLLTDDGRWSFNIISPQYQCEKVYRVQLRDPLDSEATAAIATTFAAGLQLQGE
TALTLPATLQQVDEKTVLLTLTEGKFHQVKRMFAAVGNKVIALHRAQIGAVVLDVDIGEWRELTLAEIDSFTA
>Mature_233_residues
MRLDKFLCKSTDLDRDAASVAIINGSVTVNGTIATDCAMQVHENNAVCLNGKPLAPRPSRYFMLHKPADTLCSNVDGAYP
SLFHLLDIEHAFDLHIVGRLDADTTGLVLLTDDGRWSFNIISPQYQCEKVYRVQLRDPLDSEATAAIATTFAAGLQLQGE
TALTLPATLQQVDEKTVLLTLTEGKFHQVKRMFAAVGNKVIALHRAQIGAVVLDVDIGEWRELTLAEIDSFTA

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=46.7811158798283, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=29.2372881355932, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=28.8702928870293, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25494; Mature: 25494

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLCKSTDLDRDAASVAIINGSVTVNGTIATDCAMQVHENNAVCLNGKPLAPRPSR
CCHHHHHHCCCCCCCCCCEEEEEECCEEECCEEEEEEEEEEECCCEEEECCCCCCCCCCE
YFMLHKPADTLCSNVDGAYPSLFHLLDIEHAFDLHIVGRLDADTTGLVLLTDDGRWSFNI
EEEEECCHHHHHCCCCCCCHHHHHHEECCCCEEEEEEEEECCCCCEEEEEECCCCEEEEE
ISPQYQCEKVYRVQLRDPLDSEATAAIATTFAAGLQLQGETALTLPATLQQVDEKTVLLT
ECCCHHHCEEEEEEECCCCCCCHHHHHHHHHHHCEEECCCEEEECHHHHHHCCCCEEEEE
LTEGKFHQVKRMFAAVGNKVIALHRAQIGAVVLDVDIGEWRELTLAEIDSFTA
EECCHHHHHHHHHHHHCCEEEEEEECEEEEEEEEECCCCCCEEEHHHHCCCCC
>Mature Secondary Structure
MRLDKFLCKSTDLDRDAASVAIINGSVTVNGTIATDCAMQVHENNAVCLNGKPLAPRPSR
CCHHHHHHCCCCCCCCCCEEEEEECCEEECCEEEEEEEEEEECCCEEEECCCCCCCCCCE
YFMLHKPADTLCSNVDGAYPSLFHLLDIEHAFDLHIVGRLDADTTGLVLLTDDGRWSFNI
EEEEECCHHHHHCCCCCCCHHHHHHEECCCCEEEEEEEEECCCCCEEEEEECCCCEEEEE
ISPQYQCEKVYRVQLRDPLDSEATAAIATTFAAGLQLQGETALTLPATLQQVDEKTVLLT
ECCCHHHCEEEEEEECCCCCCCHHHHHHHHHHHCEEECCCEEEECHHHHHHCCCCEEEEE
LTEGKFHQVKRMFAAVGNKVIALHRAQIGAVVLDVDIGEWRELTLAEIDSFTA
EECCHHHHHHHHHHHHCCEEEEEEECEEEEEEEEECCCCCCEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]