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The map label for this gene is mscL

Identifier: 160877075

GI number: 160877075

Start: 4675850

End: 4676260

Strand: Direct

Name: mscL

Synonym: Sbal195_3971

Alternate gene names: 160877075

Gene position: 4675850-4676260 (Clockwise)

Preceding gene: 160877074

Following gene: 160877083

Centisome position: 87.44

GC content: 46.47

Gene sequence:

>411_bases
ATGAGCTTAATTAAAGAGTTCAAGGCTTTTGCGTCCCGTGGCAATGTGATCGACATGGCCGTTGGTATCATCATAGGTGC
CGCATTCGGCAAGATAGTCTCGTCCTTTGTGGCCGATATCATAATGCCACCGATTGGTATCATTTTAGGCGGAGTGAATT
TTAGTGATTTGAGCATAGTGCTGCAGGCAGCTCAAGGCGACGCGCCTTCTGTGGTGATTGCCTATGGTAAATTCATTCAA
ACCATTATCGACTTTACGATTATCGCCTTCGCCATTTTCATGGGCGTCAAAGCAATCAATCGCTTAAAGCGCAAAGAAGA
AGTCGCGCCTAAAGCACCCGCGGCACCGACTAAAGATCAAGAACTGCTGTCTGAGATCCGCGATCTACTGAAAGCTCAGC
AAGAAAAATAA

Upstream 100 bases:

>100_bases
TAAGCTTGGCAAGGTAACAGGCAGCAAAAGACCTTAGGTTTTCCTGATACAGTTTGCATAATAAAATTTAACATTTAATC
TCAATTTTGGAGGAAACCTA

Downstream 100 bases:

>100_bases
GCCTAATCTAAAGCGCAGATCATAAAAAACCCATCGAACTCGATGGGTTTTGTTTTTATATCGGCGATGTTTCACATCAA
GCAGATAAGCGATTACTCAG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIVLQAAQGDAPSVVIAYGKFIQ
TIIDFTIIAFAIFMGVKAINRLKRKEEVAPKAPAAPTKDQELLSEIRDLLKAQQEK

Sequences:

>Translated_136_residues
MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIVLQAAQGDAPSVVIAYGKFIQ
TIIDFTIIAFAIFMGVKAINRLKRKEEVAPKAPAAPTKDQELLSEIRDLLKAQQEK
>Mature_135_residues
SLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIVLQAAQGDAPSVVIAYGKFIQT
IIDFTIIAFAIFMGVKAINRLKRKEEVAPKAPAAPTKDQELLSEIRDLLKAQQEK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=62.5, Blast_Score=178, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_SHEB2 (B8ECI1)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002359689.1
- ProteinModelPortal:   B8ECI1
- SMR:   B8ECI1
- GeneID:   7087453
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_3789
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14576; Mature: 14444

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x22063b7c)-; HASH(0x2248add8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LQAAQGDAPSVVIAYGKFIQTIIDFTIIAFAIFMGVKAINRLKRKEEVAPKAPAAPTKDQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
ELLSEIRDLLKAQQEK
HHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LQAAQGDAPSVVIAYGKFIQTIIDFTIIAFAIFMGVKAINRLKRKEEVAPKAPAAPTKDQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
ELLSEIRDLLKAQQEK
HHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA