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The map label for this gene is mdh [H]

Identifier: 160876913

GI number: 160876913

Start: 4489406

End: 4490341

Strand: Reverse

Name: mdh [H]

Synonym: Sbal195_3809

Alternate gene names: 160876913

Gene position: 4490341-4489406 (Counterclockwise)

Preceding gene: 160876915

Following gene: 160876911

Centisome position: 83.97

GC content: 49.15

Gene sequence:

>936_bases
ATGAAAGTTGCTGTACTTGGTGCTGCTGGTGGTATCGGCCAGGCTCTTGCCCTACTGTTAAAAACTCAACTGCCTGCGGG
TTCACATTTGTCTCTATATGATATCGCACCGGTTACTCCGGGTGTTGCGGTTGACTTGAGCCACATCCCTACTGCTGTTG
AAATCAAAGGTTTTGCTGGTGAAGATCCAACGCCTGCCCTAGTGGGTGCTGACGTTGTTCTAATTTCTGCTGGTGTTGCG
CGTAAGCCTGGTATGGATCGTTCAGATCTGTTCAATATCAATGCCGGTATCGTTCGCAACCTGATCGAAAAAGTAGCGGC
AACTTGCCCTACGGCTTTGGTTGGCATTATCACTAACCCAGTCAACACCACTGTCGCTATCGCTGCAGAAGTGATGAAGA
AAGCGGGTGTTTATGATAAGAACCGTCTGTTCGGTATCACGACATTAGACGTGATCCGTTCTGAAACCTTCATCGCCGAA
TTAAAAAGCCTGAACGTTGCTGACGTTAAAGTCAATGTGATCGGCGGCCACAGCGGCGTGACCATTCTGCCATTACTGTC
TCAAGTTGAAGGCGTGACTTTCACTGACGAAGAAGTGGCAAGTCTGACTACTCGCATCCAAAACGCGGGTACAGAAGTGG
TTGAAGCTAAAGCCGGTGGCGGTAGCGCGACTCTGTCTATGGGTCAAGCGGCTTGCCGTTTCGGTCTGTCTTTGGTTCGT
GGTCTGCAAGGCGAAGCTAACATCGTTGAATGCGCCTATGTTGACGGCGGCAGCGAGCACGCAGAGTTCTTCGCACAACC
TGTGCTGTTAGGCAAAAACGGTATTGAAAAAGTACTGCCTTACGGTGAAGTCAGTGCATTCGAAGCTAACGCTCGTGATT
CTATGTTAGATACGCTGAAAGGCGATATCAAACTAGGCGTTGATTTCGTTAAATAA

Upstream 100 bases:

>100_bases
CGACTAAGGGATAATTGAAATTGTTGTGACATTCCAGTAATGTGACGCCAACGTAAATTAGATCCATGTCACACATATCC
GAGAAATAACGGAGATAACT

Downstream 100 bases:

>100_bases
TCTTTCGGATAATCTTTTAGATTATGACGAATTAAAAAACGGAGCCATTGAGCTCCGTTTTTTTATGCCTGGGTTTTATA
CTTTCTAAAGCTCATTCAGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAE
LKSLNVADVKVNVIGGHSGVTILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAE
LKSLNVADVKVNVIGGHSGVTILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAE
LKSLNVADVKVNVIGGHSGVTILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=55.4487179487179, Blast_Score=321, Evalue=5e-88,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=79.3548387096774, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=56.8690095846645, Blast_Score=325, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=50.1587301587302, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6320125, Length=345, Percent_Identity=36.8115942028986, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324446, Length=367, Percent_Identity=35.9673024523161, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=53.8709677419355, Blast_Score=314, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=47.6038338658147, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=44.6666666666667, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32104; Mature: 32104

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALVGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNP
CCCCCHHHCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAELKSLNVADVKVNVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCC
TILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCEECHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCCEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIKLGVDFVK
CCEEECEEECC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALVGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNP
CCCCCHHHCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAELKSLNVADVKVNVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCC
TILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCEECHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCCEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIKLGVDFVK
CCEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA