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The map label for this gene is metE [H]

Identifier: 160876867

GI number: 160876867

Start: 4432934

End: 4435204

Strand: Reverse

Name: metE [H]

Synonym: Sbal195_3763

Alternate gene names: 160876867

Gene position: 4435204-4432934 (Counterclockwise)

Preceding gene: 160876869

Following gene: 160876865

Centisome position: 82.94

GC content: 48.75

Gene sequence:

>2271_bases
ATGCAATTAAATAGTCTTGGGTTCCCCCGTATCGGGCGTCGTCGTGAATTGAAATTTGCCTTAGAAAAATACTGGCGTGG
TGAGATCACTCAAGCTGAACTGCGTGAAGTGGCGAGCGAACTGCGCCGTACCCATTGGCAGTGGCAAACCGCTGCTGGCA
TCGAGCAAGTGCCCGTGGGGGATTTTGCCTTTTACGATCAAGTGCTTACCTTAAGTGCGACCTTAAATGCCATTCCTGAG
CGTCACCGCGGTGAAGGCGCTATCGATCTCGATACTCTATTCCGTGTTGCCCGCGGCAGAGCGCCAACGGGCCAAGATGC
GCCAGCTTCTGAAATGACCAAGTATTTCAATACTAACTATCACTATCTGGTGCCTGAGCTGACGCAAAACCAAGAGTTTT
CGATTGCTTACGAGCAGTTTTTTGATGAAGTCAGCGAAGCGCAAGCGCTAGGTTATAAAGCCAAACCTGTTCTACTCGGC
CCAGTCAGTTACTTGCACCTTGCTAAAACTGTGGGTCAAGAGTTCGATAAGTTAAGTTTATTACCTAATTTATTAACTGC
TTATGCTGACATTTTGGCGCGTTTCGCCGTCCAAGGTGTGGAATGGGTACAGTTAGATGAGCCTGTGTTGGCATTAGAGT
TAGACACGCATTGGCAAGCGGCGATGACTAAGACTTATCAAGCATTAAGCACTGCTAACGTTAAGGTTCTCTTAGCGAGT
TATTATGGCGGTATTGCGCATCATCAAGCCCTCGTATCTGCCTTGCCTGTTGCTGGTTTGCACTTAGATTTAGTTACTGC
GCCAAAACAACTGGCTGTGTTTGCAAATGCGTTGCGCAGTGAGCAAATTCTGTCGGCGGGTGTGGTTAATGGTCGTAACG
TGTGGGCGGCGGATATCGATTTGATTGCCGAGCGTATTGGCGCTGTGGCACGTGATTTAGGCGATCGTCTGTGGATTGGC
ACATCATGTTCATTACTGCATAGCCCAGTCGATTTAGATGTTGAAGTCAAACTATTACCTGTACTGCGTAAGCAGTTGGC
CTTTGCTAAGCAGAAACTGTTGGAATTGAGCAAAATTCGCCAATTATTGTTGGCGCCTAATTCGGATGAAGCACAAAAAA
TCAGTGCTTTTTGTATCGCTCGTCGTCAAGCCAAAGCGCAGGCTGCAGATAAGCAAGTGATTGATCGTGTTGCGGCATTA
ACTGCTGCGGATTATGAGCGTGTCAGTGATTTCAGTGTACGCCAAAGTGTGCAGCAGCAAAAATACCGTTTACCCCTGTT
GCCAACCACGACCATAGGTTCGTTCCCGCAAACACCCGCTATTCGAGGATTACGCAGCCGCTGGCGTAAGGGCGAGATAA
GTGATGCTCAGTATACTGAGCAGTTACAACAAGTGACCCGCGATACTATCGAACGCCAACTAAAACTGGGTATCGACGTA
CTCGTGCACGGTGAAGCCGAACGTAACGACATGGTGGAATACTTTGGCGAGCAGTTAGCTGGCGTTGGTTTTAGTAAAAA
TGGCTGGGTACAAAGTTATGGCTCACGTTGTGTTAAGCCACCCTTGATTTACGGCGATGTGAGTCGTCCAAAAGCCATGA
CAGTCGATTGGGCAAAGTTTGCTCAAAGCCTGACTGACAAACCCGTAAAAGGCATGTTGACGGGGCCAGTGACGATTTTG
CACTGGTCATTCGCCCGTGAAGATATCAGCCGCGATGCGATTGCAACACAACTGGCGCTGGCGATTCGTGATGAAGTAGT
CGATTTACAAAATGCGGGGATTGGCATTATTCAAATAGATGAGCCCGCCTTCCGTGAAGGTTTGCCATTGAAGCAAAGTG
AGTGGCAAACCTATTTAGATTGGGCGGTGAATGCCTTTAAACTCAGCGCTGCTGGCGTGACTGATGAGACTCAAATCCAC
ACTCACATGTGTTACAGCGAGTTTAACGACACGATTGCCGCGATTGCTGCCATGGATGCAGATGTGATCACAATCGAGAC
GTCGCGTTCGCGGATGGAGTTACTCAACGCCTTTGAAGATTTTGAATATCCAAATGAAATCGGCCCCGGTGTGTATGACA
TCCACTCACCAAACATCCCAAGTGTGGAGGCCATGGTGCAGTTAATCGAGAAAGCCGCGCAGAAAGTCCCCGTGCGTCAA
TTATGGGTCAACCCAGATTGTGGCCTAAAAACCCGAACTTGGGATGAAGTCGAACCTGCATTGAAAAACATGGTTGATGC
TACCCGCGAACTGCGTCGCCGCCTTGGCTAA

Upstream 100 bases:

>100_bases
GTTATTTATCAAGGCGGAAATGCTGAAAAGTGCCAAGAGAACACAATCTGGTGGGTGCAAGTGCCGAATTTGGCTTATCT
CAAATAAAGGAAGATAGGGT

Downstream 100 bases:

>100_bases
TATCACTGCTTGAGTAAGAGTTTTTAACTGAACTTATTGAGCTTACTCAGTAAAAAGACACCGGATGTTTATGCCTCCGG
TGTCTTTTATTCGCTGCGAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MQLNSLGFPRIGRRRELKFALEKYWRGEITQAELREVASELRRTHWQWQTAAGIEQVPVGDFAFYDQVLTLSATLNAIPE
RHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNYHYLVPELTQNQEFSIAYEQFFDEVSEAQALGYKAKPVLLG
PVSYLHLAKTVGQEFDKLSLLPNLLTAYADILARFAVQGVEWVQLDEPVLALELDTHWQAAMTKTYQALSTANVKVLLAS
YYGGIAHHQALVSALPVAGLHLDLVTAPKQLAVFANALRSEQILSAGVVNGRNVWAADIDLIAERIGAVARDLGDRLWIG
TSCSLLHSPVDLDVEVKLLPVLRKQLAFAKQKLLELSKIRQLLLAPNSDEAQKISAFCIARRQAKAQAADKQVIDRVAAL
TAADYERVSDFSVRQSVQQQKYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGEISDAQYTEQLQQVTRDTIERQLKLGIDV
LVHGEAERNDMVEYFGEQLAGVGFSKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAKFAQSLTDKPVKGMLTGPVTIL
HWSFAREDISRDAIATQLALAIRDEVVDLQNAGIGIIQIDEPAFREGLPLKQSEWQTYLDWAVNAFKLSAAGVTDETQIH
THMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNIPSVEAMVQLIEKAAQKVPVRQ
LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG

Sequences:

>Translated_756_residues
MQLNSLGFPRIGRRRELKFALEKYWRGEITQAELREVASELRRTHWQWQTAAGIEQVPVGDFAFYDQVLTLSATLNAIPE
RHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNYHYLVPELTQNQEFSIAYEQFFDEVSEAQALGYKAKPVLLG
PVSYLHLAKTVGQEFDKLSLLPNLLTAYADILARFAVQGVEWVQLDEPVLALELDTHWQAAMTKTYQALSTANVKVLLAS
YYGGIAHHQALVSALPVAGLHLDLVTAPKQLAVFANALRSEQILSAGVVNGRNVWAADIDLIAERIGAVARDLGDRLWIG
TSCSLLHSPVDLDVEVKLLPVLRKQLAFAKQKLLELSKIRQLLLAPNSDEAQKISAFCIARRQAKAQAADKQVIDRVAAL
TAADYERVSDFSVRQSVQQQKYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGEISDAQYTEQLQQVTRDTIERQLKLGIDV
LVHGEAERNDMVEYFGEQLAGVGFSKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAKFAQSLTDKPVKGMLTGPVTIL
HWSFAREDISRDAIATQLALAIRDEVVDLQNAGIGIIQIDEPAFREGLPLKQSEWQTYLDWAVNAFKLSAAGVTDETQIH
THMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNIPSVEAMVQLIEKAAQKVPVRQ
LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG
>Mature_756_residues
MQLNSLGFPRIGRRRELKFALEKYWRGEITQAELREVASELRRTHWQWQTAAGIEQVPVGDFAFYDQVLTLSATLNAIPE
RHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNYHYLVPELTQNQEFSIAYEQFFDEVSEAQALGYKAKPVLLG
PVSYLHLAKTVGQEFDKLSLLPNLLTAYADILARFAVQGVEWVQLDEPVLALELDTHWQAAMTKTYQALSTANVKVLLAS
YYGGIAHHQALVSALPVAGLHLDLVTAPKQLAVFANALRSEQILSAGVVNGRNVWAADIDLIAERIGAVARDLGDRLWIG
TSCSLLHSPVDLDVEVKLLPVLRKQLAFAKQKLLELSKIRQLLLAPNSDEAQKISAFCIARRQAKAQAADKQVIDRVAAL
TAADYERVSDFSVRQSVQQQKYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGEISDAQYTEQLQQVTRDTIERQLKLGIDV
LVHGEAERNDMVEYFGEQLAGVGFSKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAKFAQSLTDKPVKGMLTGPVTIL
HWSFAREDISRDAIATQLALAIRDEVVDLQNAGIGIIQIDEPAFREGLPLKQSEWQTYLDWAVNAFKLSAAGVTDETQIH
THMCYSEFNDTIAAIAAMDADVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNIPSVEAMVQLIEKAAQKVPVRQ
LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=56.3829787234042, Blast_Score=863, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=762, Percent_Identity=45.0131233595801, Blast_Score=643, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84490; Mature: 84490

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNSLGFPRIGRRRELKFALEKYWRGEITQAELREVASELRRTHWQWQTAAGIEQVPVG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DFAFYDQVLTLSATLNAIPERHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNY
HHHHHHHHHHHHHHHHHCCHHHCCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
HYLVPELTQNQEFSIAYEQFFDEVSEAQALGYKAKPVLLGPVSYLHLAKTVGQEFDKLSL
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
LPNLLTAYADILARFAVQGVEWVQLDEPVLALELDTHWQAAMTKTYQALSTANVKVLLAS
HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHH
YYGGIAHHQALVSALPVAGLHLDLVTAPKQLAVFANALRSEQILSAGVVNGRNVWAADID
HHCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHH
LIAERIGAVARDLGDRLWIGTSCSLLHSPVDLDVEVKLLPVLRKQLAFAKQKLLELSKIR
HHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QLLLAPNSDEAQKISAFCIARRQAKAQAADKQVIDRVAALTAADYERVSDFSVRQSVQQQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGEISDAQYTEQLQQVTRDTIERQLKLGIDV
HCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
LVHGEAERNDMVEYFGEQLAGVGFSKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAKF
EEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCCCEEEEHHHHH
AQSLTDKPVKGMLTGPVTILHWSFAREDISRDAIATQLALAIRDEVVDLQNAGIGIIQID
HHHHCCCCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
EPAFREGLPLKQSEWQTYLDWAVNAFKLSAAGVTDETQIHTHMCYSEFNDTIAAIAAMDA
CHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNIPSVEAMVQLIEKAAQKVPVRQ
CEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCHH
LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLNSLGFPRIGRRRELKFALEKYWRGEITQAELREVASELRRTHWQWQTAAGIEQVPVG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DFAFYDQVLTLSATLNAIPERHRGEGAIDLDTLFRVARGRAPTGQDAPASEMTKYFNTNY
HHHHHHHHHHHHHHHHHCCHHHCCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
HYLVPELTQNQEFSIAYEQFFDEVSEAQALGYKAKPVLLGPVSYLHLAKTVGQEFDKLSL
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
LPNLLTAYADILARFAVQGVEWVQLDEPVLALELDTHWQAAMTKTYQALSTANVKVLLAS
HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHH
YYGGIAHHQALVSALPVAGLHLDLVTAPKQLAVFANALRSEQILSAGVVNGRNVWAADID
HHCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHH
LIAERIGAVARDLGDRLWIGTSCSLLHSPVDLDVEVKLLPVLRKQLAFAKQKLLELSKIR
HHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QLLLAPNSDEAQKISAFCIARRQAKAQAADKQVIDRVAALTAADYERVSDFSVRQSVQQQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGEISDAQYTEQLQQVTRDTIERQLKLGIDV
HCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
LVHGEAERNDMVEYFGEQLAGVGFSKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAKF
EEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCCCEEEEHHHHH
AQSLTDKPVKGMLTGPVTILHWSFAREDISRDAIATQLALAIRDEVVDLQNAGIGIIQID
HHHHCCCCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
EPAFREGLPLKQSEWQTYLDWAVNAFKLSAAGVTDETQIHTHMCYSEFNDTIAAIAAMDA
CHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNIPSVEAMVQLIEKAAQKVPVRQ
CEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCHH
LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]