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The map label for this gene is ackA [H]

Identifier: 160875883

GI number: 160875883

Start: 3302052

End: 3303251

Strand: Direct

Name: ackA [H]

Synonym: Sbal195_2772

Alternate gene names: 160875883

Gene position: 3302052-3303251 (Clockwise)

Preceding gene: 160875881

Following gene: 160875884

Centisome position: 61.75

GC content: 46.58

Gene sequence:

>1200_bases
ATGTCAAATAATCTGGTTTTAGTTCTCAATTGCGGTAGCTCTTCTCTCAAATTTGCCGTCATTGACGCGCAAACAGGTGA
CGATCAAATTTCTGGCTTAGCCGAATGTTTTGGTTTAGAAGACTCGCGCATTAAATGGAAAATCAACGGCGAAAAACATG
AAGCGGCCTTAGGCGCATTTACGGCTCACCGTGAAGCAGTTGAATACATTGTTAATAAAATCCTTGCGGAACAACCTGAA
TTAGCAGCAAAAATTCAAGCCGTTGGCCACCGTATCGTTCACGGCGGTGAAAAGTTCACTCGTTCTGTTATCATCGACGA
AAGCGTGATCAAAGGTATCGAAGATTGTGCTTCACTAGCCCCCCTGCACAATCCTGCTCACCTTATCGGCATCCGTGCAG
CCATTGCCTCTTTCCCTAAATTGCCACAGGTTGCCGTATTTGATACCGCTTTCCACCAAAGCATGCCAGACAGAGCTTAT
GTCTACGCCCTGCCTTACAAGTTGTACCGTGAACACGGTATCCGTCGTTATGGCATGCATGGCACGAGCCATTTATTCGT
TAGCCGTGAAGCGGCAAAAATGCTCAACAAGCCCATTGAAGAAACTAACGTTATCTGTGCTCACCTGGGCAACGGCGCGT
CAGTGACTGCCATCAAAGGCGGCAAAAGTGTCGACACGTCTATGGGCTTAACCCCATTAGAAGGTTTGGTCATGGGCACA
CGTTGTGGCGATATTGACCCTTCAATCATCTATCACTTAGTTCATCAACTGGGTTACACCTTAGAAGAAGTGAATAACCT
GATGAACAAGCAAAGTGGTCTACTGGGGATTTCCGAGCTAACTAACGATTGCCGCGGTATCGAAGAAGGTTACGCAGACG
GTCATAAAGGCGCGACCTTAGCCTTAGAAATCTTCTGCTACCGTTTAGCCAAATACATCGCCTCTTACACTGTGCCACTG
GGCCGTTTAGATGCAGTCGTCTTCACCGGCGGCATTGGTGAAAACTCAGACTTAATCCGTGAAAAAGTACTCAACATGCT
GGAAATCTTCAATTTCCATGTCGATAGCGAACGCAACAAAGCGGCGCGTTTCGGTAAGAAAGGCATCATCACTCAAGATA
AAGGCACGATTGCCATGGTGATCCCAACGAACGAAGAATGGGTTATCGCCGAAGATTCAATTAAACTGATCAACAAATAA

Upstream 100 bases:

>100_bases
CACAATCTGGCGAATCGCCCCAAGACTGTTATGGTAAAAACACCGCAGATCCTAATTTTTTTGAATCGTTATATTAAACA
CAATGGCAAAGGGTTTACAC

Downstream 100 bases:

>100_bases
AAAATGACCTAAGCCGTTTGCTCACCCAAACGGCTTTACGCTTTACATAGCCATACTCACAAGCAAATTTGCAGAAACTC
TTCTGCCAAAAACCATCGAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAFTAHREAVEYIVNKILAEQPE
LAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAY
VYALPYKLYREHGIRRYGMHGTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPL
GRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNKAARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK

Sequences:

>Translated_399_residues
MSNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAFTAHREAVEYIVNKILAEQPE
LAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAY
VYALPYKLYREHGIRRYGMHGTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPL
GRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNKAARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK
>Mature_398_residues
SNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAFTAHREAVEYIVNKILAEQPEL
AAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAYV
YALPYKLYREHGIRRYGMHGTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGTR
CGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPLG
RLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNKAARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=64.6616541353383, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=45.5470737913486, Blast_Score=342, Evalue=3e-95,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43601; Mature: 43469

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAF
CCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
TAHREAVEYIVNKILAEQPELAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHC
PLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAYVYALPYKLYREHGIRRYGMH
CCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCC
GTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
CCCEEEEEHHHHHHHCCCCHHHCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCHH
ALEIFCYRLAKYIASYTVPLGRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNK
HHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK
HHHCCCCCCEEECCCCEEEEEECCCCEEEECCCHHHCCC
>Mature Secondary Structure 
SNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAF
CCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
TAHREAVEYIVNKILAEQPELAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHC
PLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAYVYALPYKLYREHGIRRYGMH
CCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCC
GTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
CCCEEEEEHHHHHHHCCCCHHHCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCHH
ALEIFCYRLAKYIASYTVPLGRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNK
HHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK
HHHCCCCCCEEECCCCEEEEEECCCCEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA