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The map label for this gene is rlmL [H]

Identifier: 160875561

GI number: 160875561

Start: 2895777

End: 2896784

Strand: Reverse

Name: rlmL [H]

Synonym: Sbal195_2449

Alternate gene names: 160875561

Gene position: 2896784-2895777 (Counterclockwise)

Preceding gene: 160875562

Following gene: 160875556

Centisome position: 54.17

GC content: 48.31

Gene sequence:

>1008_bases
ATGTCTATTCAAACGCTGTTATCGACGGCTCTGGCGAAACGCCATGATTTTTTACAACAAGCAGAACAGGATCAAACCGA
TTGTTTTCGGGTGTTCCATGGCACAGTAGAAGGCGTGAGTGGCTTGAATGTCGACCGTTACGGCGATGCTTGGCTAGTGC
AGAGTTTTCATCAGACCTTAACCGATGAAGAGTTAGCCGATGTTAGTGCCGTTTTGCTGCAACAGGCCGATTTGCCGATT
GTGTATAACGACCGCTCATTAGGCAATTCCCGCGTATTGAACGCGTTATCACCCGCACTGACAGAGATCGCGAGTTCTGA
GCGAGTCATGCAGGAAAATGGCATCAAGTTCAGCACTAAACTGCGTCATGAAGGGCAAGATCCGCTGTTATTCCTCGATA
TGCGCATTGGTCGTGAATATGTACGGGCAAACAGCCAAGATAAAACCGTGCTGAATTTGTTTTCTTACACCTGCGGCATT
GGTACAGCAGCAGCCATCGGCGGCGCGACTCGGGTGATGAACGTCGATTTTTCTTCCTTTGCTTTAGCCACGGGCAAGGC
GAATGCGGCGTTAAATCAGGTGTCCAATGTGTGTGAGTTTGTGCAGAGTGATGCGTTTCCGGCATTGCGCCAATTGGCTG
GTTTAAGTGTGGGAGGGCGACGCAATCAAAAATTACCGAGCTACCCAAAATTGCCGCAAACCCAGTTTGATCTCGTGTTC
CTCGATCCGCCGCGTTATGCCAAGAGTGCTTTCGGTATTGTCGATTTGATCAATGATTATCAAGGTTTATTTAAGCCTGC
ATTAATGGCGACTAAAGTCGGCGGCACTATCGTTTGCTGCAACAATGTGGCGAAAGTTGAGCGCCAAGCATGGTTTGACA
GTTTAGTACGCTGCGTTGAAAAACAAGGTCGTACAGTCACAGCGCACACTTGGCTTGATCCCCATGAAGATTTCCCTTCG
TTCGATGACAATCATCCACTTAAAATTGTCGCGCTGACCTTAAGTTAA

Upstream 100 bases:

>100_bases
TTTGGTGAATTTATCCGATATTCTGCGACCTAGTTTGTTATTATGCCGTTTTCCTTCTTGAAACCTTACGCAGGTTTCCG
CTGCGCGAGTCCCGCTTTTT

Downstream 100 bases:

>100_bases
CGGCATAGCGATACGAACGGCATAGTGATACCAACAAACAGGGCACCCGATTAGGTGCCCTTTTTATTGTCCATTTATTT
GCTGCTATATGGGTTGCAAG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSIQTLLSTALAKRHDFLQQAEQDQTDCFRVFHGTVEGVSGLNVDRYGDAWLVQSFHQTLTDEELADVSAVLLQQADLPI
VYNDRSLGNSRVLNALSPALTEIASSERVMQENGIKFSTKLRHEGQDPLLFLDMRIGREYVRANSQDKTVLNLFSYTCGI
GTAAAIGGATRVMNVDFSSFALATGKANAALNQVSNVCEFVQSDAFPALRQLAGLSVGGRRNQKLPSYPKLPQTQFDLVF
LDPPRYAKSAFGIVDLINDYQGLFKPALMATKVGGTIVCCNNVAKVERQAWFDSLVRCVEKQGRTVTAHTWLDPHEDFPS
FDDNHPLKIVALTLS

Sequences:

>Translated_335_residues
MSIQTLLSTALAKRHDFLQQAEQDQTDCFRVFHGTVEGVSGLNVDRYGDAWLVQSFHQTLTDEELADVSAVLLQQADLPI
VYNDRSLGNSRVLNALSPALTEIASSERVMQENGIKFSTKLRHEGQDPLLFLDMRIGREYVRANSQDKTVLNLFSYTCGI
GTAAAIGGATRVMNVDFSSFALATGKANAALNQVSNVCEFVQSDAFPALRQLAGLSVGGRRNQKLPSYPKLPQTQFDLVF
LDPPRYAKSAFGIVDLINDYQGLFKPALMATKVGGTIVCCNNVAKVERQAWFDSLVRCVEKQGRTVTAHTWLDPHEDFPS
FDDNHPLKIVALTLS
>Mature_334_residues
SIQTLLSTALAKRHDFLQQAEQDQTDCFRVFHGTVEGVSGLNVDRYGDAWLVQSFHQTLTDEELADVSAVLLQQADLPIV
YNDRSLGNSRVLNALSPALTEIASSERVMQENGIKFSTKLRHEGQDPLLFLDMRIGREYVRANSQDKTVLNLFSYTCGIG
TAAAIGGATRVMNVDFSSFALATGKANAALNQVSNVCEFVQSDAFPALRQLAGLSVGGRRNQKLPSYPKLPQTQFDLVFL
DPPRYAKSAFGIVDLINDYQGLFKPALMATKVGGTIVCCNNVAKVERQAWFDSLVRCVEKQGRTVTAHTWLDPHEDFPSF
DDNHPLKIVALTLS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=276, Percent_Identity=26.8115942028986, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787181, Length=286, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 36932; Mature: 36801

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQTLLSTALAKRHDFLQQAEQDQTDCFRVFHGTVEGVSGLNVDRYGDAWLVQSFHQTL
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TDEELADVSAVLLQQADLPIVYNDRSLGNSRVLNALSPALTEIASSERVMQENGIKFSTK
CHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LRHEGQDPLLFLDMRIGREYVRANSQDKTVLNLFSYTCGIGTAAAIGGATRVMNVDFSSF
CCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCHHHH
ALATGKANAALNQVSNVCEFVQSDAFPALRQLAGLSVGGRRNQKLPSYPKLPQTQFDLVF
EEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
LDPPRYAKSAFGIVDLINDYQGLFKPALMATKVGGTIVCCNNVAKVERQAWFDSLVRCVE
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHH
KQGRTVTAHTWLDPHEDFPSFDDNHPLKIVALTLS
HCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
SIQTLLSTALAKRHDFLQQAEQDQTDCFRVFHGTVEGVSGLNVDRYGDAWLVQSFHQTL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TDEELADVSAVLLQQADLPIVYNDRSLGNSRVLNALSPALTEIASSERVMQENGIKFSTK
CHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LRHEGQDPLLFLDMRIGREYVRANSQDKTVLNLFSYTCGIGTAAAIGGATRVMNVDFSSF
CCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCHHHH
ALATGKANAALNQVSNVCEFVQSDAFPALRQLAGLSVGGRRNQKLPSYPKLPQTQFDLVF
EEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
LDPPRYAKSAFGIVDLINDYQGLFKPALMATKVGGTIVCCNNVAKVERQAWFDSLVRCVE
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHH
KQGRTVTAHTWLDPHEDFPSFDDNHPLKIVALTLS
HCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA