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The map label for this gene is thiE [H]

Identifier: 160875550

GI number: 160875550

Start: 2881838

End: 2883736

Strand: Reverse

Name: thiE [H]

Synonym: Sbal195_2438

Alternate gene names: 160875550

Gene position: 2883736-2881838 (Counterclockwise)

Preceding gene: 160875551

Following gene: 160875549

Centisome position: 53.93

GC content: 48.71

Gene sequence:

>1899_bases
ATGAGTACCGAGCGTCCCGCGTTTGTTTGGACCATAGCTGGTTCAGACAGTGGCGGTGGTGCCGGTATTCAGGCGGACTT
AGCGACAATTCAAGATTTGGGCTGTCACGGTTGCAGTGTGGTCACCACAGTGACGGCGCAAAGTTCTGTGGCGGTCACTT
TAGTCGAGCCCGTATCAGCGGCGATGTTAATGGCTCAGCTGACGACTTTACTTTCCGACTTGCCACCCAAAGCAATTAAA
ATCGGCTTACTGGCAGATCAAACTCAAGTGGCATTGCTGGCAGATTGGATCGCGAGTTTTAAAATCCACTATCCATCTGT
GCCTGTGATTGTCGATCCTGTGATGGTCGCCAGTTGTGGCGATGCATTAGCAGTAGATAACTGTCAGGATATAAAAAGTG
CGGCCAAATCAGCCTTAGATTTTAGGCCTTTCAAAGGTTTAATCGAACTTATCACGCCCAATGTGCTTGAACTTGGGCGA
TTAACTCACAGTGATGTTTCAACGAAAGCGCAATTCGCTGCCGCGGCACTGGCTTTATCCCAGAGTCTTGATTGCAGTGT
GCTCGCCAAAGGGGGCGATGTAAGCTTTGGCAGCACTGACATTCTTGAAAATACTCATGCTAAAACTCACGATAATACTT
ATGCTCAAACTCAGGCTAACGCTCACAATAGCAACGGCTGGGATCTTGAGCTTGCCGAGGATTATCTGGTTTGCCATCAA
GTACGCGCGAGCTCTAAACTGCATCAAAATGGGCGTTTCTGGTTAGCCAGCCAGCGGGTTAATACCCGTCATAACCATGG
TAGCGGCTGTACTTTGTCATCGGCCATTGCCGCCGTGTTAGCGCAGGGTTTTGTATTACAAGATGCGGTGGTGGTTGCCA
AAGCCTATGTGAGTCAAGGCTTAAGCGCGGCGATTGGCTTAGGACAAGGTCCAGGGCCGTTGGCCCGTACGGGTTGGCCT
AATAACTTGTCCCGCTATGCCAAGATAAATCTGTGTGATGGCAATTTTATTCGTCATCACCTCAATCGACATCTTGATGT
TAGAAGTGATTTAGTTGCAACAGTTTTATCCGCAACAGATCAGGCAACCGCTCAGGTAAGAATAGCCTCGACGCCACCTC
AAAATATTTTATCCCACGGTTTTAAAGTGCTCGATGCCGAGCTTGGTGTTTATCCCGTGGTGAGTGACTTAACCATGCTG
GAAAGTTTATTAGCGGCAGGCGTTAAAACTGTGCAGTTACGGATAAAAACCGACATCAGCGAGTTAACTACTACCACCGC
GCCAGCCGAATCTGATTTAGGCAAGTCTGCGCTAGGTAGATGTGAGTCAGGCGAACCTGAGTTAATTGGCTCCGAATTAG
AAGCACAAATTCAAACAGCCATTGCCTTAGGCAAGCATTTTAATGCACAGCTTTTTATTAATGATCACTGGAAGTTAGCG
ATAAAATACCATGCCTTCGGGGTACATTTAGGCCAAGAAGATCTCGCCGTTACTGACTTAGCGGCCATTCAAGCCGCGGG
GCTCGCGCTAGGAATATCGAGCCACAGTTATTTCGAGTTGCTATTGGCGCACCAATACTCGCCATCCTACATAGCGCTTG
GGCATATATTCCCAACCACGACGAAGCAAATGCCTTCGGCGCCCCAAGGGCTCGCAAAACTTAAACACTATGTGGCGTTA
CTCCAAGGCCATTATCCCTTGGTCGCCATTGGCGGTATTGACTTAACAAATCTGGCAAAGGTGAAAGCAACAGGGGTGGG
CAATATTGCTGTGGTGCGCGCAATAACGAAAGCTAAGGAGCCAGTCGCAGCGTTTGCAGAGCTGAGCCAAGCTTGGGAGC
AATGTAGCTTGTGTGAAGAACTGGCTGTAAAGCAAGAGTTGGATGCAAAGCATGAGTAA

Upstream 100 bases:

>100_bases
TTGCTGCTTCGGGCGCCGAGATCTATCACGTGAAAGACAAGCTTGTCGCCGAAGCTGAGGAAGCCTAATTATGCAGGGTA
TTCATGGGGGGAAATTGCCC

Downstream 100 bases:

>100_bases
AAAAACACCTCTTTCTCACGCAGAGGTTCTTTGCGGTCATATACACAGCGACAATGAACACTGCGGCCATGCACTCAGTG
ATGCCGATTTCATCCGTTAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MSTERPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSAAMLMAQLTTLLSDLPPKAIK
IGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCGDALAVDNCQDIKSAAKSALDFRPFKGLIELITPNVLELGR
LTHSDVSTKAQFAAAALALSQSLDCSVLAKGGDVSFGSTDILENTHAKTHDNTYAQTQANAHNSNGWDLELAEDYLVCHQ
VRASSKLHQNGRFWLASQRVNTRHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQGLSAAIGLGQGPGPLARTGWP
NNLSRYAKINLCDGNFIRHHLNRHLDVRSDLVATVLSATDQATAQVRIASTPPQNILSHGFKVLDAELGVYPVVSDLTML
ESLLAAGVKTVQLRIKTDISELTTTTAPAESDLGKSALGRCESGEPELIGSELEAQIQTAIALGKHFNAQLFINDHWKLA
IKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTTTKQMPSAPQGLAKLKHYVAL
LQGHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKEPVAAFAELSQAWEQCSLCEELAVKQELDAKHE

Sequences:

>Translated_632_residues
MSTERPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSAAMLMAQLTTLLSDLPPKAIK
IGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCGDALAVDNCQDIKSAAKSALDFRPFKGLIELITPNVLELGR
LTHSDVSTKAQFAAAALALSQSLDCSVLAKGGDVSFGSTDILENTHAKTHDNTYAQTQANAHNSNGWDLELAEDYLVCHQ
VRASSKLHQNGRFWLASQRVNTRHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQGLSAAIGLGQGPGPLARTGWP
NNLSRYAKINLCDGNFIRHHLNRHLDVRSDLVATVLSATDQATAQVRIASTPPQNILSHGFKVLDAELGVYPVVSDLTML
ESLLAAGVKTVQLRIKTDISELTTTTAPAESDLGKSALGRCESGEPELIGSELEAQIQTAIALGKHFNAQLFINDHWKLA
IKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTTTKQMPSAPQGLAKLKHYVAL
LQGHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKEPVAAFAELSQAWEQCSLCEELAVKQELDAKHE
>Mature_631_residues
STERPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSAAMLMAQLTTLLSDLPPKAIKI
GLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCGDALAVDNCQDIKSAAKSALDFRPFKGLIELITPNVLELGRL
THSDVSTKAQFAAAALALSQSLDCSVLAKGGDVSFGSTDILENTHAKTHDNTYAQTQANAHNSNGWDLELAEDYLVCHQV
RASSKLHQNGRFWLASQRVNTRHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQGLSAAIGLGQGPGPLARTGWPN
NLSRYAKINLCDGNFIRHHLNRHLDVRSDLVATVLSATDQATAQVRIASTPPQNILSHGFKVLDAELGVYPVVSDLTMLE
SLLAAGVKTVQLRIKTDISELTTTTAPAESDLGKSALGRCESGEPELIGSELEAQIQTAIALGKHFNAQLFINDHWKLAI
KYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTTTKQMPSAPQGLAKLKHYVALL
QGHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKEPVAAFAELSQAWEQCSLCEELAVKQELDAKHE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=220, Percent_Identity=51.8181818181818, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788420, Length=308, Percent_Identity=30.1948051948052, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325378, Length=306, Percent_Identity=28.1045751633987, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324997, Length=202, Percent_Identity=29.2079207920792, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324517, Length=209, Percent_Identity=29.1866028708134, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 66916; Mature: 66785

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTERPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSA
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECHHHH
AMLMAQLTTLLSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCG
HHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCEEECCCCCEEECCHHHHCCC
DALAVDNCQDIKSAAKSALDFRPFKGLIELITPNVLELGRLTHSDVSTKAQFAAAALALS
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHH
QSLDCSVLAKGGDVSFGSTDILENTHAKTHDNTYAQTQANAHNSNGWDLELAEDYLVCHQ
CCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEHHHHHHHHHH
VRASSKLHQNGRFWLASQRVNTRHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQG
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
LSAAIGLGQGPGPLARTGWPNNLSRYAKINLCDGNFIRHHLNRHLDVRSDLVATVLSATD
CHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCC
QATAQVRIASTPPQNILSHGFKVLDAELGVYPVVSDLTMLESLLAAGVKTVQLRIKTDIS
CCEEEEEEECCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCHH
ELTTTTAPAESDLGKSALGRCESGEPELIGSELEAQIQTAIALGKHFNAQLFINDHWKLA
HHHCCCCCCHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEE
IKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTT
EEEEEEEEEECCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCC
TKQMPSAPQGLAKLKHYVALLQGHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKE
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHEEEECCCCCHHHHHHHHHCCC
PVAAFAELSQAWEQCSLCEELAVKQELDAKHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STERPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSA
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECHHHH
AMLMAQLTTLLSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCG
HHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCEEECCCCCEEECCHHHHCCC
DALAVDNCQDIKSAAKSALDFRPFKGLIELITPNVLELGRLTHSDVSTKAQFAAAALALS
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHH
QSLDCSVLAKGGDVSFGSTDILENTHAKTHDNTYAQTQANAHNSNGWDLELAEDYLVCHQ
CCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEHHHHHHHHHH
VRASSKLHQNGRFWLASQRVNTRHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQG
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
LSAAIGLGQGPGPLARTGWPNNLSRYAKINLCDGNFIRHHLNRHLDVRSDLVATVLSATD
CHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCC
QATAQVRIASTPPQNILSHGFKVLDAELGVYPVVSDLTMLESLLAAGVKTVQLRIKTDIS
CCEEEEEEECCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCHH
ELTTTTAPAESDLGKSALGRCESGEPELIGSELEAQIQTAIALGKHFNAQLFINDHWKLA
HHHCCCCCCHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEE
IKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTT
EEEEEEEEEECCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCC
TKQMPSAPQGLAKLKHYVALLQGHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKE
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHEEEECCCCCHHHHHHHHHCCC
PVAAFAELSQAWEQCSLCEELAVKQELDAKHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA