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The map label for this gene is thiF [H]

Identifier: 160875549

GI number: 160875549

Start: 2880934

End: 2881854

Strand: Reverse

Name: thiF [H]

Synonym: Sbal195_2437

Alternate gene names: 160875549

Gene position: 2881854-2880934 (Counterclockwise)

Preceding gene: 160875550

Following gene: 160875548

Centisome position: 53.89

GC content: 47.56

Gene sequence:

>921_bases
ATGCAAAGCATGAGTAAAAAAACACCTCTTTCTCACGCAGAGGTTCTTTGCGGTCATATACACAGCGACAATGAACACTG
CGGCCATGCACTCAGTGATGCCGATTTCATCCGTTATTCTCGGCAAGTCTTATTGCCAGAAGTGGGTGAGGCTGGGCAAT
TACAACTGGCCGACGCCAGCGTTGTTATTATCGGTCTTGGCGGTTTAGGGCAATTGGCGGCGCAGTATCTGGCTTGTGCA
GGTATTGGTCGCTTGACCTTAATCGATAGGGATAAGGTTGAAGTATCGAATCTGCCAAGACAATTGTTATTCAACGATGC
CGATATTGGGCTCAATAAAGCGCAAATTGCCAAGCAAAAGCTCAATCGCATAGCACCGCATTGCACTGTTACTGCCCATG
AAACAGCATTTAATGCTGCGACATCGGCTCATCATTTAGCGGATATTTTACAAATAAAACAACAAGGTAAAAGAGTACTT
GTACTTGACTGCACCGATAACTTTGCGACTCGCCAAGCCATTAATCGCAGCTGTATCGAAGCGGCTTTAACTTTAGTCAG
CGCGTCAATCGCGGCATTTAGCGGCCAATTGTTCGCGGTCGACCAAATGCAGTTCCCCTCTGGTGGTTGTTATCACTGTA
TTTTCCCCGCACAAACGAGAGTGTCGCAGAGCTGCAGTACCCAAGGCGTACTCGGGCCGAGCGTAGGCGTCATGGCGTCA
ATGCAATCTTTGGTGGCCATGCAACTCTTGTTGAATGTGGATAGTTGTGATGAACCTAAAAGTGCCTTGTTGGGACGTTT
TTGGCGCTTCGACGCTAAATCACTTTCATGGACAGCGGCGATATTAACGCGGGATCCCCATTGTGACGTATGTGGTCCAA
AAGAGGTTCATTCATTATCCGATAAGCCCAGCAAACTATAA

Upstream 100 bases:

>100_bases
ATAACGAAAGCTAAGGAGCCAGTCGCAGCGTTTGCAGAGCTGAGCCAAGCTTGGGAGCAATGTAGCTTGTGTGAAGAACT
GGCTGTAAAGCAAGAGTTGG

Downstream 100 bases:

>100_bases
AGTATGGAGGTTTGATATGATTTTAATTCACATAAATAATGTCCCGCAGCCATTAACGGAACCGACTTCACTCGCGGCTG
TGATCCTCGCGCAAGATATA

Product: UBA/THIF-type NAD/FAD-binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQSMSKKTPLSHAEVLCGHIHSDNEHCGHALSDADFIRYSRQVLLPEVGEAGQLQLADASVVIIGLGGLGQLAAQYLACA
GIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKAQIAKQKLNRIAPHCTVTAHETAFNAATSAHHLADILQIKQQGKRVL
VLDCTDNFATRQAINRSCIEAALTLVSASIAAFSGQLFAVDQMQFPSGGCYHCIFPAQTRVSQSCSTQGVLGPSVGVMAS
MQSLVAMQLLLNVDSCDEPKSALLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSLSDKPSKL

Sequences:

>Translated_306_residues
MQSMSKKTPLSHAEVLCGHIHSDNEHCGHALSDADFIRYSRQVLLPEVGEAGQLQLADASVVIIGLGGLGQLAAQYLACA
GIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKAQIAKQKLNRIAPHCTVTAHETAFNAATSAHHLADILQIKQQGKRVL
VLDCTDNFATRQAINRSCIEAALTLVSASIAAFSGQLFAVDQMQFPSGGCYHCIFPAQTRVSQSCSTQGVLGPSVGVMAS
MQSLVAMQLLLNVDSCDEPKSALLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSLSDKPSKL
>Mature_306_residues
MQSMSKKTPLSHAEVLCGHIHSDNEHCGHALSDADFIRYSRQVLLPEVGEAGQLQLADASVVIIGLGGLGQLAAQYLACA
GIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKAQIAKQKLNRIAPHCTVTAHETAFNAATSAHHLADILQIKQQGKRVL
VLDCTDNFATRQAINRSCIEAALTLVSASIAAFSGQLFAVDQMQFPSGGCYHCIFPAQTRVSQSCSTQGVLGPSVGVMAS
MQSLVAMQLLLNVDSCDEPKSALLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSLSDKPSKL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=187, Percent_Identity=43.3155080213904, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4885649, Length=176, Percent_Identity=27.8409090909091, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI13376212, Length=265, Percent_Identity=29.811320754717, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI38327032, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI38045944, Length=84, Percent_Identity=42.8571428571429, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI38045942, Length=84, Percent_Identity=42.8571428571429, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87082356, Length=262, Percent_Identity=40.8396946564886, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787048, Length=263, Percent_Identity=37.2623574144487, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1789177, Length=101, Percent_Identity=39.6039603960396, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=267, Percent_Identity=34.4569288389513, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17508819, Length=228, Percent_Identity=25.8771929824561, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI193203301, Length=207, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=267, Percent_Identity=32.2097378277154, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6322639, Length=177, Percent_Identity=24.8587570621469, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24582879, Length=281, Percent_Identity=36.2989323843416, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24660640, Length=172, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24641311, Length=248, Percent_Identity=31.4516129032258, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20129973, Length=73, Percent_Identity=49.3150684931507, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 32900; Mature: 32900

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSMSKKTPLSHAEVLCGHIHSDNEHCGHALSDADFIRYSRQVLLPEVGEAGQLQLADAS
CCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCE
VVIIGLGGLGQLAAQYLACAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKAQIAKQK
EEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCHHHHCCCCCCCCCHHHHHHHH
LNRIAPHCTVTAHETAFNAATSAHHLADILQIKQQGKRVLVLDCTDNFATRQAINRSCIE
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
AALTLVSASIAAFSGQLFAVDQMQFPSGGCYHCIFPAQTRVSQSCSTQGVLGPSVGVMAS
HHHHHHHHHHHHHCCCEEEEECEECCCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHHH
MQSLVAMQLLLNVDSCDEPKSALLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSLS
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHCC
DKPSKL
CCCCCC
>Mature Secondary Structure
MQSMSKKTPLSHAEVLCGHIHSDNEHCGHALSDADFIRYSRQVLLPEVGEAGQLQLADAS
CCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCE
VVIIGLGGLGQLAAQYLACAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKAQIAKQK
EEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCHHHHCCCCCCCCCHHHHHHHH
LNRIAPHCTVTAHETAFNAATSAHHLADILQIKQQGKRVLVLDCTDNFATRQAINRSCIE
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
AALTLVSASIAAFSGQLFAVDQMQFPSGGCYHCIFPAQTRVSQSCSTQGVLGPSVGVMAS
HHHHHHHHHHHHHCCCEEEEECEECCCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHHH
MQSLVAMQLLLNVDSCDEPKSALLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSLS
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHCC
DKPSKL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]