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The map label for this gene is polB [H]

Identifier: 160874770

GI number: 160874770

Start: 1994542

End: 1996971

Strand: Direct

Name: polB [H]

Synonym: Sbal195_1653

Alternate gene names: 160874770

Gene position: 1994542-1996971 (Clockwise)

Preceding gene: 160874763

Following gene: 160874771

Centisome position: 37.3

GC content: 47.24

Gene sequence:

>2430_bases
ATGAAGCCTGAGGCAACAACGGCCGTTAGTGTACAAGGTCGAGTCTTAACACGCCATGCGATTCATCGTCATGGGAGTTT
AGTGTTGCAGTATTATCTGGCGACCGCCCAAGGTCCTGTGCTGGTGGAAGTGCAAGGCACTGAACACATTTGTTTTTGTC
ATCAAACGGATGTGGCGGCTTTACAGTTACAAACGCCGGGTCAAGCATTGCGATTTGTGCCATTAGCGCTCAAAAGCTTT
AAAGGCGGCTCCGTCAGTGGAGTTTATGCTAAATCGAGCAACAGCTTACGTACCTTACAGCGCATCGCAAAAGATGCCGA
TATCCCTTTATTTGAAGCGGATATTCGCCCTGAACAGCGTTATTTAATCGAGCGGTTTGTGGCGCTGGATGTGGCATTTT
TAGGGCATTTCTCTGCGCCGACTAGCGACAGTCTTGAGGCTAACTCGCTGCCCGTGTTTATCGCCGAGCGGGCTAAGAGT
ATTGCCCCTTTAACGGCGGTGAATTTACGCTGTATCTCACTGGATTTTGAATGCAGCTTTGAGGGCTTACTCTATTCGGT
GGCGCTATATGGTCAAGACAGTGGCCGAGACGAGGGGCAAGGCAGCCTCAAGCAGACTTATGAAAAAGTGATTATGGTGG
GCGAAGCTGAATCAAACACCCCCGAATATATTGAATGGGTGGCCGATGAAGCGGCGCTGATCCATCGATTAATCGCGTGG
TTCCATGAGTTTGATCCTGACTTTATTATCGGTTGGTCAGTGGTGAGTTTCGATCTCGCGCTATTGTCACGCCGTGCTCT
ATTACACCGTATTCCGCTCACCATTGGCCGCGGTGGCGCTAAGCTCGATTGGAAAGTTGACAATAAGTTTCGCCCAGAAA
CCTTAGTTTTGCCGGGCCGTGTGGTACTCGATGGTATCGATTGGCTTAAAGCCGCTTTTTATCACTTTGAGCGTTTTTCG
CTTGAATTTGTGTCACGGCACCTATTAGGTGAAGGCAAGGCCATTCATAACGTCGATAATCGCGTTGAAGAAATTGACCA
GCTGTTTAAGACCGATAAACAAGCCTTAGCGCATTACAATCTTACCGATAGCCGCCTAGTGTGGGATATTTTTACTCACA
CTCAATTGTGGGATTTTGCCCTCGCGCGTGCTGAACTGACAGGTTTAGAACTGGGACGGGTAGGGGCCTCAGTGGCGGCG
TTTAATCATTTGTATTTACCGCATCTACATCGCGCGGGTTATGTCGCTCCTGCAGAGCCTGTGAGTCAGGGCATTGAAAG
TCCTGGTGGTTACGTGATGGATTCTGTGCCGGGTCTTTATAAACACATCTTAGTGTTCGATTTTAAAAGTCTGTATCCCT
CCATTATTCGCACCTTTTTGATCGATCCTAAGGGATTGATTGAAGGCTTGCAGTTGCCGGAGTCTGACTGTGTTGCGGGG
TTTCTCGGCGGCCGTTTCAGTCGGCAACAGCCTATTTTACCTAAGCTTATCCAAACCTTATCTGAGCAGCGAGAAAAGGC
CAAGCGTGAGCACAATGCGCCGTTATCCCAAGCGATAAAAATTATCATGAACTCGCTGTATGGGGTTTTAGGTTCTCAAG
GTTGTGTGTTCCACGATGCAAAGTTAGCAAGTTCGATCACTATGCGCGGCCATCAAATCATGAAACAAACCCGTGCATGG
ATAGAAGAGATGGGCTATCAAGTGATTTATGGCGATACGGATTCGACCTTTGTCTGGCTTGGCAGCGAGCCTGATCTCTC
AGACATTAACGCCTTGGGTAACATTCTGGCGCAGGATATCAATGCTAAGTGGCAGGCTAAAATCTGTGCTGATCACCAAG
TTGAGAGTTTTCTCGAGTTACAGTTTGAGCGGCATTACGAGCAGTTCTTTATGCCAACCTTAAGAGGCTCATTAGAAGGC
AGTAAAAAGCGTTATGTTGGCGCGTGGCGCAATGCGAGCGATGAACTTGAGCTCACCTTTAAAGGTATGGAACAAGTGCG
CAGTGATTGGAGCCCCTTAGCGCGCAAAGTCCAAGAGTCACTCTATGAGCGAATGTTTAACCAAAGGGATATCAGTGTTT
ATTTAGCCAGCGTGTTTGAAAAATTACTTGATGGTCAGTTGGATAATGATTTGGTATTTCGTAAACGCATGCGGCGCAAT
ATCGAAGATTACACGGCTAAATCTTCACCACATGTTAAAGTCGCCCAGCAACTATGCGACGTAACAGGTGACACCACTTT
TGGCAAGCATGGGGCGCAAATTGACTACCTGATTACTGTAAATGGACCGGAACCCTTACTGTACAGAACGTCACCAATTG
ATTACCAATACTACATTGATAAGCAAATTGGCCCTATTGCAGAGCCGGTATTTTCGATAATGAAACTAAACTACATGGAT
ATTGCGTCAAAGCAGTTGCTGTTAATATAG

Upstream 100 bases:

>100_bases
TAATGCTGTACATAATAACAGTGATTGCATAGCCTAAGAAACTTTTCTGCCATTTGGCGGCATTTAAATTGAGTAGCTAT
ACGATTTGGGAACCCATGCG

Downstream 100 bases:

>100_bases
TGTTTTTAATGTTCTTTATGTGTTGCCATAAGTGGCGAATGTAGCGTTTTGACCTGTTTTGTAGAGGTTAACTGGTTTGT
TTTTGTGATGTTAATCGACA

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
MKPEATTAVSVQGRVLTRHAIHRHGSLVLQYYLATAQGPVLVEVQGTEHICFCHQTDVAALQLQTPGQALRFVPLALKSF
KGGSVSGVYAKSSNSLRTLQRIAKDADIPLFEADIRPEQRYLIERFVALDVAFLGHFSAPTSDSLEANSLPVFIAERAKS
IAPLTAVNLRCISLDFECSFEGLLYSVALYGQDSGRDEGQGSLKQTYEKVIMVGEAESNTPEYIEWVADEAALIHRLIAW
FHEFDPDFIIGWSVVSFDLALLSRRALLHRIPLTIGRGGAKLDWKVDNKFRPETLVLPGRVVLDGIDWLKAAFYHFERFS
LEFVSRHLLGEGKAIHNVDNRVEEIDQLFKTDKQALAHYNLTDSRLVWDIFTHTQLWDFALARAELTGLELGRVGASVAA
FNHLYLPHLHRAGYVAPAEPVSQGIESPGGYVMDSVPGLYKHILVFDFKSLYPSIIRTFLIDPKGLIEGLQLPESDCVAG
FLGGRFSRQQPILPKLIQTLSEQREKAKREHNAPLSQAIKIIMNSLYGVLGSQGCVFHDAKLASSITMRGHQIMKQTRAW
IEEMGYQVIYGDTDSTFVWLGSEPDLSDINALGNILAQDINAKWQAKICADHQVESFLELQFERHYEQFFMPTLRGSLEG
SKKRYVGAWRNASDELELTFKGMEQVRSDWSPLARKVQESLYERMFNQRDISVYLASVFEKLLDGQLDNDLVFRKRMRRN
IEDYTAKSSPHVKVAQQLCDVTGDTTFGKHGAQIDYLITVNGPEPLLYRTSPIDYQYYIDKQIGPIAEPVFSIMKLNYMD
IASKQLLLI

Sequences:

>Translated_809_residues
MKPEATTAVSVQGRVLTRHAIHRHGSLVLQYYLATAQGPVLVEVQGTEHICFCHQTDVAALQLQTPGQALRFVPLALKSF
KGGSVSGVYAKSSNSLRTLQRIAKDADIPLFEADIRPEQRYLIERFVALDVAFLGHFSAPTSDSLEANSLPVFIAERAKS
IAPLTAVNLRCISLDFECSFEGLLYSVALYGQDSGRDEGQGSLKQTYEKVIMVGEAESNTPEYIEWVADEAALIHRLIAW
FHEFDPDFIIGWSVVSFDLALLSRRALLHRIPLTIGRGGAKLDWKVDNKFRPETLVLPGRVVLDGIDWLKAAFYHFERFS
LEFVSRHLLGEGKAIHNVDNRVEEIDQLFKTDKQALAHYNLTDSRLVWDIFTHTQLWDFALARAELTGLELGRVGASVAA
FNHLYLPHLHRAGYVAPAEPVSQGIESPGGYVMDSVPGLYKHILVFDFKSLYPSIIRTFLIDPKGLIEGLQLPESDCVAG
FLGGRFSRQQPILPKLIQTLSEQREKAKREHNAPLSQAIKIIMNSLYGVLGSQGCVFHDAKLASSITMRGHQIMKQTRAW
IEEMGYQVIYGDTDSTFVWLGSEPDLSDINALGNILAQDINAKWQAKICADHQVESFLELQFERHYEQFFMPTLRGSLEG
SKKRYVGAWRNASDELELTFKGMEQVRSDWSPLARKVQESLYERMFNQRDISVYLASVFEKLLDGQLDNDLVFRKRMRRN
IEDYTAKSSPHVKVAQQLCDVTGDTTFGKHGAQIDYLITVNGPEPLLYRTSPIDYQYYIDKQIGPIAEPVFSIMKLNYMD
IASKQLLLI
>Mature_809_residues
MKPEATTAVSVQGRVLTRHAIHRHGSLVLQYYLATAQGPVLVEVQGTEHICFCHQTDVAALQLQTPGQALRFVPLALKSF
KGGSVSGVYAKSSNSLRTLQRIAKDADIPLFEADIRPEQRYLIERFVALDVAFLGHFSAPTSDSLEANSLPVFIAERAKS
IAPLTAVNLRCISLDFECSFEGLLYSVALYGQDSGRDEGQGSLKQTYEKVIMVGEAESNTPEYIEWVADEAALIHRLIAW
FHEFDPDFIIGWSVVSFDLALLSRRALLHRIPLTIGRGGAKLDWKVDNKFRPETLVLPGRVVLDGIDWLKAAFYHFERFS
LEFVSRHLLGEGKAIHNVDNRVEEIDQLFKTDKQALAHYNLTDSRLVWDIFTHTQLWDFALARAELTGLELGRVGASVAA
FNHLYLPHLHRAGYVAPAEPVSQGIESPGGYVMDSVPGLYKHILVFDFKSLYPSIIRTFLIDPKGLIEGLQLPESDCVAG
FLGGRFSRQQPILPKLIQTLSEQREKAKREHNAPLSQAIKIIMNSLYGVLGSQGCVFHDAKLASSITMRGHQIMKQTRAW
IEEMGYQVIYGDTDSTFVWLGSEPDLSDINALGNILAQDINAKWQAKICADHQVESFLELQFERHYEQFFMPTLRGSLEG
SKKRYVGAWRNASDELELTFKGMEQVRSDWSPLARKVQESLYERMFNQRDISVYLASVFEKLLDGQLDNDLVFRKRMRRN
IEDYTAKSSPHVKVAQQLCDVTGDTTFGKHGAQIDYLITVNGPEPLLYRTSPIDYQYYIDKQIGPIAEPVFSIMKLNYMD
IASKQLLLI

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=631, Percent_Identity=26.9413629160063, Blast_Score=140, Evalue=6e-33,
Organism=Homo sapiens, GI106507301, Length=661, Percent_Identity=25.5673222390318, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI153792012, Length=671, Percent_Identity=22.8017883755589, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1786246, Length=808, Percent_Identity=42.450495049505, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI32565317, Length=629, Percent_Identity=25.1192368839428, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17559488, Length=718, Percent_Identity=22.9805013927577, Blast_Score=119, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320101, Length=692, Percent_Identity=24.8554913294798, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324227, Length=545, Percent_Identity=22.5688073394495, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325090, Length=434, Percent_Identity=24.8847926267281, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24664937, Length=646, Percent_Identity=25.6965944272446, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648780, Length=638, Percent_Identity=22.7272727272727, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 91180; Mature: 91180

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00116 DNA_POLYMERASE_B ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEATTAVSVQGRVLTRHAIHRHGSLVLQYYLATAQGPVLVEVQGTEHICFCHQTDVAA
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEE
LQLQTPGQALRFVPLALKSFKGGSVSGVYAKSSNSLRTLQRIAKDADIPLFEADIRPEQR
EEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCCHHHH
YLIERFVALDVAFLGHFSAPTSDSLEANSLPVFIAERAKSIAPLTAVNLRCISLDFECSF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHCCCCCEEEEEEEEEEEECCCCC
EGLLYSVALYGQDSGRDEGQGSLKQTYEKVIMVGEAESNTPEYIEWVADEAALIHRLIAW
CHHEEEEEEECCCCCCCCCCCHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
FHEFDPDFIIGWSVVSFDLALLSRRALLHRIPLTIGRGGAKLDWKVDNKFRPETLVLPGR
HHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCEEEECCH
VVLDGIDWLKAAFYHFERFSLEFVSRHLLGEGKAIHNVDNRVEEIDQLFKTDKQALAHYN
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTDSRLVWDIFTHTQLWDFALARAELTGLELGRVGASVAAFNHLYLPHLHRAGYVAPAEP
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHH
VSQGIESPGGYVMDSVPGLYKHILVFDFKSLYPSIIRTFLIDPKGLIEGLQLPESDCVAG
HHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHH
FLGGRFSRQQPILPKLIQTLSEQREKAKREHNAPLSQAIKIIMNSLYGVLGSQGCVFHDA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHH
KLASSITMRGHQIMKQTRAWIEEMGYQVIYGDTDSTFVWLGSEPDLSDINALGNILAQDI
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHC
NAKWQAKICADHQVESFLELQFERHYEQFFMPTLRGSLEGSKKRYVGAWRNASDELELTF
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCCCCEEEEH
KGMEQVRSDWSPLARKVQESLYERMFNQRDISVYLASVFEKLLDGQLDNDLVFRKRMRRN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
IEDYTAKSSPHVKVAQQLCDVTGDTTFGKHGAQIDYLITVNGPEPLLYRTSPIDYQYYID
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCEEEEEC
KQIGPIAEPVFSIMKLNYMDIASKQLLLI
CCCCHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MKPEATTAVSVQGRVLTRHAIHRHGSLVLQYYLATAQGPVLVEVQGTEHICFCHQTDVAA
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEE
LQLQTPGQALRFVPLALKSFKGGSVSGVYAKSSNSLRTLQRIAKDADIPLFEADIRPEQR
EEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCCHHHH
YLIERFVALDVAFLGHFSAPTSDSLEANSLPVFIAERAKSIAPLTAVNLRCISLDFECSF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHCCCCCEEEEEEEEEEEECCCCC
EGLLYSVALYGQDSGRDEGQGSLKQTYEKVIMVGEAESNTPEYIEWVADEAALIHRLIAW
CHHEEEEEEECCCCCCCCCCCHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
FHEFDPDFIIGWSVVSFDLALLSRRALLHRIPLTIGRGGAKLDWKVDNKFRPETLVLPGR
HHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCEEEECCH
VVLDGIDWLKAAFYHFERFSLEFVSRHLLGEGKAIHNVDNRVEEIDQLFKTDKQALAHYN
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTDSRLVWDIFTHTQLWDFALARAELTGLELGRVGASVAAFNHLYLPHLHRAGYVAPAEP
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHH
VSQGIESPGGYVMDSVPGLYKHILVFDFKSLYPSIIRTFLIDPKGLIEGLQLPESDCVAG
HHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHH
FLGGRFSRQQPILPKLIQTLSEQREKAKREHNAPLSQAIKIIMNSLYGVLGSQGCVFHDA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHH
KLASSITMRGHQIMKQTRAWIEEMGYQVIYGDTDSTFVWLGSEPDLSDINALGNILAQDI
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHC
NAKWQAKICADHQVESFLELQFERHYEQFFMPTLRGSLEGSKKRYVGAWRNASDELELTF
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCCCCEEEEH
KGMEQVRSDWSPLARKVQESLYERMFNQRDISVYLASVFEKLLDGQLDNDLVFRKRMRRN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
IEDYTAKSSPHVKVAQQLCDVTGDTTFGKHGAQIDYLITVNGPEPLLYRTSPIDYQYYID
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCEEEEEC
KQIGPIAEPVFSIMKLNYMDIASKQLLLI
CCCCHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]