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The map label for this gene is pleD [H]

Identifier: 160874516

GI number: 160874516

Start: 1673216

End: 1674772

Strand: Reverse

Name: pleD [H]

Synonym: Sbal195_1398

Alternate gene names: 160874516

Gene position: 1674772-1673216 (Counterclockwise)

Preceding gene: 160874517

Following gene: 160874515

Centisome position: 31.32

GC content: 43.67

Gene sequence:

>1557_bases
ATGAAGGATTCGAGCGCTACAGTTTCACAACTTGCCTTGCTGCAACAGAAACTTCATTCGGCCAAACTTGCCCTCGATGA
AGTCAATGAAGATAGAAATGCAAAGTTACAGACATTACTGCAATTTATTGGTCACTTAAGCCTCGCCTGCAAAGGGCAAA
ATCTTGAGCTGGATAATAAACTCGCCAAACTTCGCCATAACTTAAATACGTTTGAGCGAGTCGATGAGGCGTTGCCAGAA
TTAGTCGATGTCGAGCGCCTGTTAAAAGGCCAATATCACCACGTGATGGTACAACTCGAAGAGAGTCGTACGGGTTTGTC
ACAGGTGATCCGTCAAATCCAACGCGTTAACTCTGCACCTGAAAAACTCAAAAAAGAAGTCAATTACTTCAAACAAGATC
TGGCTAAACCTTTCCACACGGTTTGGGAATATATTCCTAAAGTTAAGCAAATCATCGGCTTCTACGAAACCATATTACAG
CTACAATTTAACGAATCAGAAAAACTCGATGTCTTGCCTAAGCACAGGCAGCTAGCCCACGAACTGGCGCAGATGATTTC
AGAAATCGAGTTCCGTAAAGATCAGCGTGACCAAGTCCTAGTGATCAAAGAAGTGCTCTCTTATGAGATTGAAGTTGATA
GCTTGCTCGAAGCCTACCAAACCATTTTAGCCTTATTGCTTGATAACATCGCCCGCGAGAAATCGGCATCGCAAGAGTTT
TTGTTTGCCCTCAACGATGCCTTAGCCGCAGTGCGCGAAGTAGTCAGTGAGTCTTACAACCACAATCAACGCAGCTTCCA
ACTCAAGACCCAGTTAAATCGTGAGATTAATACCCGCGTCGATAACGCCGGTGAAATCATTATCGATGTCGAAGATGTCG
TCGATCTTAAAGCCCAATTAACTGAGCAACTTAACTCCATTCGTTCGGCATTGGCCCGTAAAGAAGCCTTAGAGCAAAGA
GAACAGGCCTTACTGCGTAAATCAATGGAAACAATGCGCAAAGAGCTTAATGAATTAAGTAATGAGGCCAATACCTACAA
AGAGCGATTGTTTGAACAACAAAAACTCAACTTGCTCGATAGCTTAACTCAATTGCCTAACCGTGCCGCGTTAGAAGAGC
GCATGGATTTGGAATACCGCAACTATCAACGGCATAAATCTCCACTTTGGGTGGCGGTTGCCGATATTGACCACTTCAAA
ACCATCAACGACAGCTTTGGCCATAGCACGGGTGATAAAACCTTGCAGGTGATTGCAATGGCATTGAAAAACTCACTACG
AGATACCGAATTTGTGGCCCGTTATGGCGGCGAAGAATTTGTCCTGTTATTACCTGATGTCGCACCTGCTGATATCGCTC
AACTTTTAAACAGAGTGCGCGAGAAAGTAAAAAATATTCCATTTAAATTTAAAAATCAGAGAATTACAGTTACAGTATCT
ATAGGGGCTGCGCAAGTTATGGGAAGTGAGCTCATACATGAGACCTTCGAACGCGCCGATGCAGCACTCTATAAAGCAAA
ACACGAAAGCAGAGACAGGGTTGTGATTGACGTATAA

Upstream 100 bases:

>100_bases
ACGCCCAGTGTTATAACATTAGTATCATATGAGTTAACAGCTAATCTGGACATAGGATCGTCATGTTTTTTAGCCTTGCA
GAGTTAGGACAATACTCAGG

Downstream 100 bases:

>100_bases
TTGAGCCCCACTATACGTCTCCTCTAATCACTCCTGTCATGACGAAGGAGTGATAAATGATAGTAAAAGTCAGCCCTAAG
GGCAGCATGGATCAGCTTTC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAPEKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQ
LQFNESEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKEVLSYEIEVDSLLEAYQTILALLLDNIAREKSASQEF
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQLTEQLNSIRSALARKEALEQR
EQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMDLEYRNYQRHKSPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV

Sequences:

>Translated_518_residues
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAPEKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQ
LQFNESEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKEVLSYEIEVDSLLEAYQTILALLLDNIAREKSASQEF
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQLTEQLNSIRSALARKEALEQR
EQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMDLEYRNYQRHKSPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV
>Mature_518_residues
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAPEKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQ
LQFNESEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKEVLSYEIEVDSLLEAYQTILALLLDNIAREKSASQEF
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQLTEQLNSIRSALARKEALEQR
EQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMDLEYRNYQRHKSPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.2670807453416, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=36.25, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=33.5443037974684, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=35.4430379746835, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87081881, Length=122, Percent_Identity=45.0819672131148, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=35.625, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=33.9181286549708, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=28.9940828402367, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=188, Percent_Identity=32.4468085106383, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=29.1139240506329, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=33.9622641509434, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59685; Mature: 59685

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHHH
LAKLRHNLNTFERVDEALPELVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAP
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHCHHHHHHHHHHHHCCCH
EKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQLQFNESEKLDVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
ELAQMISEIEFRKDQRDQVLVIKEVLSYEIEVDSLLEAYQTILALLLDNIAREKSASQEF
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHH
TEQLNSIRSALARKEALEQREQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTQLPNRAALEERMDLEYRNYQRHKSPLWVAVADIDHFKTINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHCCHHHHHHHCCCCEEEEEECCHHHHHHCHHCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHHH
LAKLRHNLNTFERVDEALPELVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAP
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHCHHHHHHHHHHHHCCCH
EKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQLQFNESEKLDVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
ELAQMISEIEFRKDQRDQVLVIKEVLSYEIEVDSLLEAYQTILALLLDNIAREKSASQEF
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHH
TEQLNSIRSALARKEALEQREQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTQLPNRAALEERMDLEYRNYQRHKSPLWVAVADIDHFKTINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHCCHHHHHHHCCCCEEEEEECCHHHHHHCHHCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA