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The map label for this gene is gltD [H]

Identifier: 160874373

GI number: 160874373

Start: 1489122

End: 1490528

Strand: Reverse

Name: gltD [H]

Synonym: Sbal195_1254

Alternate gene names: 160874373

Gene position: 1490528-1489122 (Counterclockwise)

Preceding gene: 160874374

Following gene: 160874372

Centisome position: 27.87

GC content: 51.53

Gene sequence:

>1407_bases
ATGAGTAACGATTTTCAATTTATTGAAGTGGGTCGCAAAGACCCCACCAAACACCCTGCGGCTAAACGTGCGACTCAGTT
TATTGAGATTTATCAGCCTTTTGCTCAGCCTGAAGTGGCACAGCAAGCGGATCGCTGTTTAGATTGCGGCAATCCTTATT
GTGAGTGGAAATGTCCGCTACACAATTATATTCCTAACTGGCTCAAGCTAGCGGAGCAAGGTCGCATCATGGAAGCGGCG
GATTTAGTGCATGAGACCAACACTCTGCCCGAAATCTGCGGCCGTGTTTGTCCACAGGACAGACTCTGCGAAGGCGCTTG
TACCCTTAACGACGACTTTGGTGCTGTCACTATCGGTAATGTCGAAAAATACATTACCGATACAGCAATCGCCCAAGGCT
GGCGCCCAGATATGAGCAAGGTCACGCCGCGCGCTGAACGAGTAGCGATTGTCGGTGCAGGTCCTGCAGGGTTGGGCTGC
GCGGATATTCTGTCTCGTAACGGCGTTAAAGCCGTGGTCTTTGATAAATATCCACAAATTGGTGGCCTACTCACCTACGG
TATTCCGTCTTTTAAACTCGACAAGGCCGTCATGGCGACCCGTCGCAGTGTATTAGAAGGCATGGGCATTGAGTTCAAAC
TCGGGGTTACTGTCGGTAAAGACATCCCATTTACTAAACTGCTCGAAGAGTACGATGCCGTATTCCTTGGTATGGGCACC
TATACCGCGATGAAAGCGGGCCTTGAGGGTGAAGATGCCCAAGGCGTATACCAAGCCCTGCCCTATTTGATTGGTAACAC
CCATCATTTAATGGGCACCCAGAATACTGAAACGCCCTACCTAAACCTTGAAGGTAAACGTGTGGTGGTATTAGGCGGTG
GTGATACTGCGATGGATTGCGTGCGCACAGCCGTCAGGCAAGGTGCCAGCAGCGTAGTCTGTGCTTACCGCCGTGACGAG
GCCAACATGCCAGGTTCACGCCGTGAAGTGCAAAATGCGCGGGAGGAAGGCGTTAACTTCCTGTTTAACCGTCAACCTGT
GGCGATTAAAACCCAAGATGGCAAAGTAGTCGGCATTGAATGCGTCGAAACCGCCATGGGCAAAGCCGATGCCAGCGGTC
GTCAACGCGCCGAAGCGATTGCGGGTAGCGAACAGTTGCTCGAAGCCGATGCGATTATTATTGCTTTCGGTTTCCAACCT
AGCCCAGCACCTTGGTTTGCCGACCATGGCATCGAGCTCGATCAATGGGGCCGTGTTAAAGCGAGTAAGTTAGACGAAAA
TCCATTCCAAACCACCAACCCAAAAGTGTTTGCCGGTGGTGATATGGTGCGCGGTTCTGACTTAGTCGTGACAGCCATTG
CCGAAGGTCGCGATGCCGCCCAAGGTATTCTTAACTACTTAGATTAA

Upstream 100 bases:

>100_bases
TTGCTTCGTATTAGTGAAACCTAAAAATATTGCCGTGGCCGATCTGCTCAAGTTAGAGAAATCGAGCCCAGAATTAGTTG
TAAAAGCGGGGTAATGGCGC

Downstream 100 bases:

>100_bases
GCGCTACAGCTTAAACGCCAATGTGTTGGTTTTTTCGCTGAAAAACTGTTTTACCGCTGTCGTTTAACAAGCACTGCAAT
GGCTGGACTTAGGCAAACAA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSNDFQFIEVGRKDPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPLHNYIPNWLKLAEQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGC
ADILSRNGVKAVVFDKYPQIGGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDCVRTAVRQGASSVVCAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGIECVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQP
SPAPWFADHGIELDQWGRVKASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD

Sequences:

>Translated_468_residues
MSNDFQFIEVGRKDPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPLHNYIPNWLKLAEQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGC
ADILSRNGVKAVVFDKYPQIGGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDCVRTAVRQGASSVVCAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGIECVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQP
SPAPWFADHGIELDQWGRVKASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
>Mature_467_residues
SNDFQFIEVGRKDPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPLHNYIPNWLKLAEQGRIMEAAD
LVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGCA
DILSRNGVKAVVFDKYPQIGGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGTY
TAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDCVRTAVRQGASSVVCAYRRDEA
NMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGIECVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQPS
PAPWFADHGIELDQWGRVKASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=447, Percent_Identity=29.3064876957494, Blast_Score=169, Evalue=7e-42,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=66.025641025641, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=460, Percent_Identity=60, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI87082180, Length=460, Percent_Identity=56.5217391304348, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=28.0898876404494, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1789243, Length=442, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=32.9243353783231, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI71984108, Length=448, Percent_Identity=30.8035714285714, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17543860, Length=205, Percent_Identity=32.6829268292683, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=32.7083333333333, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640763, Length=476, Percent_Identity=27.3109243697479, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI18858217, Length=476, Percent_Identity=27.3109243697479, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50888; Mature: 50757

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFQFIEVGRKDPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPL
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAEQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEEECC
VEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGCADILSRNGVKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCC
GGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCEEEEECCH
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDC
HHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGASSVVCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGIE
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEHH
CVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQPSPAPWFADHGIELDQWGRVK
HHHHHHCCCCCCHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
HHHCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNDFQFIEVGRKDPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPL
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAEQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEEECC
VEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGCADILSRNGVKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCC
GGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCEEEEECCH
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDC
HHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGASSVVCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGIE
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEHH
CVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQPSPAPWFADHGIELDQWGRVK
HHHHHHCCCCCCHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
HHHCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]