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The map label for this gene is cysU [H]

Identifier: 160874218

GI number: 160874218

Start: 1299866

End: 1300711

Strand: Reverse

Name: cysU [H]

Synonym: Sbal195_1098

Alternate gene names: 160874218

Gene position: 1300711-1299866 (Counterclockwise)

Preceding gene: 160874219

Following gene: 160874217

Centisome position: 24.32

GC content: 50.35

Gene sequence:

>846_bases
GTGATTTTTAATAACGGGCGTTTACGCCATAAGCGGGTTCTTCCAGGTTTTACCATCAGCTTAGGTGTGTCCCTACTGTT
TGTCAGCCTGATCCTATTATTGCCAACCACTGGGCTGATTATGCAGACCAGTTCAATGAGCTTGTCCGAATACTGGGGCG
TCATCACAGATCCTCGGGTGTTGGCGAGTTACCGAGTCACCATACTATCGGCACTCGCGGCATCGTTATTTAACTGCCTT
TTCGGATTGTTACTCGCATGGGTGCTGGTACGTTATGAATTCCCCGGCAAACGCATCTTAGATGCATTGGTGGATTTACC
CTTTGCGTTGCCGACTGCCGTTGCAGGTATCACCTTAGCGACCCTGTATGCGGAAAACGGCCAAATCGGTAGTGTATTGG
CAGAACTGGGTATAAAAGTCGCCTACACCCCGCTAGGCATAGTGGTCGCGATGATTTTCACCAGTATTCCCTTTGTGGTA
CGTACCGTGCAACCGGTTCTCGAAGAGTTTTCCCACGATGAAGAAGAAGCGGGCATGACCCTTGGCGCCTCAGATCGCGC
GGTATTCTGGCGGATCATTTTGCCGTCATTGTGGCCAGCCTTAATGGTCGGGACTGCACTGTCTTTTACCCGCAGCCTAG
GTGAGTTTGGTGCCGTCATCTTTATTGCGGGCAACATGCCCTATATCAGCGAAATCACATCGTTAATGATCTTTGTCCGC
CTGCAAGAGTTTGATTTTGCTGGTGCTAGTGCCATTGCCTCTGTGGTGCTGATGACCTCACTACTGCTATTACTGCTAAT
AAACCTGTGGCAGGCGCGTTATCTGCGCCGCATTCATGGACGATAA

Upstream 100 bases:

>100_bases
GCGAAGACGAATTCAATAGTCTTTAACTCAGACAGCCGATCGCTGACGGTAAATACAGCGTATTAAATTAACTCAACTAA
CCTGACCGGGTGATTATTCT

Downstream 100 bases:

>100_bases
GGAGCAAACATGAACTCATTTAAGCCCTTAAGAGTCGGTGAAGCACCTCTTATCAAGTGGAGCCTGATTACCCTCGCGGT
GTTCTTAGGCTTGCTATTGT

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MIFNNGRLRHKRVLPGFTISLGVSLLFVSLILLLPTTGLIMQTSSMSLSEYWGVITDPRVLASYRVTILSALAASLFNCL
FGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLATLYAENGQIGSVLAELGIKVAYTPLGIVVAMIFTSIPFVV
RTVQPVLEEFSHDEEEAGMTLGASDRAVFWRIILPSLWPALMVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
LQEFDFAGASAIASVVLMTSLLLLLLINLWQARYLRRIHGR

Sequences:

>Translated_281_residues
MIFNNGRLRHKRVLPGFTISLGVSLLFVSLILLLPTTGLIMQTSSMSLSEYWGVITDPRVLASYRVTILSALAASLFNCL
FGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLATLYAENGQIGSVLAELGIKVAYTPLGIVVAMIFTSIPFVV
RTVQPVLEEFSHDEEEAGMTLGASDRAVFWRIILPSLWPALMVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
LQEFDFAGASAIASVVLMTSLLLLLLINLWQARYLRRIHGR
>Mature_281_residues
MIFNNGRLRHKRVLPGFTISLGVSLLFVSLILLLPTTGLIMQTSSMSLSEYWGVITDPRVLASYRVTILSALAASLFNCL
FGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLATLYAENGQIGSVLAELGIKVAYTPLGIVVAMIFTSIPFVV
RTVQPVLEEFSHDEEEAGMTLGASDRAVFWRIILPSLWPALMVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
LQEFDFAGASAIASVVLMTSLLLLLLINLWQARYLRRIHGR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=272, Percent_Identity=65.4411764705882, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI87082099, Length=255, Percent_Identity=34.1176470588235, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=37.0689655172414, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=24.8847926267281, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30827; Mature: 30827

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNNGRLRHKRVLPGFTISLGVSLLFVSLILLLPTTGLIMQTSSMSLSEYWGVITDPRV
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCHHH
LASYRVTILSALAASLFNCLFGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHH
TLYAENGQIGSVLAELGIKVAYTPLGIVVAMIFTSIPFVVRTVQPVLEEFSHDEEEAGMT
HHHHCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCE
LGASDRAVFWRIILPSLWPALMVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
LQEFDFAGASAIASVVLMTSLLLLLLINLWQARYLRRIHGR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFNNGRLRHKRVLPGFTISLGVSLLFVSLILLLPTTGLIMQTSSMSLSEYWGVITDPRV
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCHHH
LASYRVTILSALAASLFNCLFGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHH
TLYAENGQIGSVLAELGIKVAYTPLGIVVAMIFTSIPFVVRTVQPVLEEFSHDEEEAGMT
HHHHCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCE
LGASDRAVFWRIILPSLWPALMVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
LQEFDFAGASAIASVVLMTSLLLLLLINLWQARYLRRIHGR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]