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The map label for this gene is cysW [H]

Identifier: 160874217

GI number: 160874217

Start: 1298990

End: 1299856

Strand: Reverse

Name: cysW [H]

Synonym: Sbal195_1097

Alternate gene names: 160874217

Gene position: 1299856-1298990 (Counterclockwise)

Preceding gene: 160874218

Following gene: 160874216

Centisome position: 24.31

GC content: 49.83

Gene sequence:

>867_bases
ATGAACTCATTTAAGCCCTTAAGAGTCGGTGAAGCACCTCTTATCAAGTGGAGCCTGATTACCCTCGCGGTGTTCTTAGG
CTTGCTATTGTTGCTATTGCCGCTGGTGAGTATTTTTCAGCAAGCGTTTGTCGGAGGTTGGGAGCGTTATATTGGTCACT
TAACTCAGCCAGATTCGCTGCATGCGATAGGTTTGACGCTGATGGTTGCGGCGCTCACCGTCCCCATCAATTTAGTGTTC
GGGATCATGCTGGCGTGGAGCGTGACTCGGTTTGAATACCCCGGTCGCAAGTTATTGATCACGCTAATTGATATTCCATT
TGCGGTCTCCCCCGTCGTCGCAGGTTTGCTCTATTTACTGCTCTATGGCAACAGTGGTTGGCTGGGTGAATGGCTATATG
AGCACGATCTGCAGATCATGTTTGCTTGGCCGGGAATACTGTTAGTCACCATTTTTGTCACTTGTTCCTTTGTCGCCAGA
GAACTCATTCCATTAATGCAACAACAGGATGCTTCAGAAGAGGAAGCCGCAGTGATCCTCGGCGCCTCATGGTGGCAACT
CTTCCGCCGAGTAACGCTCCCTAATATCAAGTGGGCACTGATTTACGGTGTGATCCTCACCAATGCCAGAGCCGTGGGCG
AATTTGGTGCTGTCGCGGTTGTCTCTGGCAACATTCGTGGCGAAACCAACACCTTACCTTTACACGTGCAGTTATTGTAC
GAAGATTATCAGGCCGAAGCCGCCTTCGCGAGCGCGTCATTACTGGCCTTGATCGCCCTATTTACCCTAATGTTAAAAGC
CGTAGTGGAATGGCGACAACAACGCAGTTTATCCGCCAATGACAATAAAGAGCAGAGCCCATTATGA

Upstream 100 bases:

>100_bases
TGCCTCTGTGGTGCTGATGACCTCACTACTGCTATTACTGCTAATAAACCTGTGGCAGGCGCGTTATCTGCGCCGCATTC
ATGGACGATAAGGAGCAAAC

Downstream 100 bases:

>100_bases
GTATTCGTCTTACTAATATTTCCAAGAAATTTGGCAAGTTTCAGGCGCTTTCCCCGCTGGATCTTGATATTCAAGAAGGG
GAAATGATTGGTCTGCTGGG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNSFKPLRVGEAPLIKWSLITLAVFLGLLLLLLPLVSIFQQAFVGGWERYIGHLTQPDSLHAIGLTLMVAALTVPINLVF
GIMLAWSVTRFEYPGRKLLITLIDIPFAVSPVVAGLLYLLLYGNSGWLGEWLYEHDLQIMFAWPGILLVTIFVTCSFVAR
ELIPLMQQQDASEEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQAEAAFASASLLALIALFTLMLKAVVEWRQQRSLSANDNKEQSPL

Sequences:

>Translated_288_residues
MNSFKPLRVGEAPLIKWSLITLAVFLGLLLLLLPLVSIFQQAFVGGWERYIGHLTQPDSLHAIGLTLMVAALTVPINLVF
GIMLAWSVTRFEYPGRKLLITLIDIPFAVSPVVAGLLYLLLYGNSGWLGEWLYEHDLQIMFAWPGILLVTIFVTCSFVAR
ELIPLMQQQDASEEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQAEAAFASASLLALIALFTLMLKAVVEWRQQRSLSANDNKEQSPL
>Mature_288_residues
MNSFKPLRVGEAPLIKWSLITLAVFLGLLLLLLPLVSIFQQAFVGGWERYIGHLTQPDSLHAIGLTLMVAALTVPINLVF
GIMLAWSVTRFEYPGRKLLITLIDIPFAVSPVVAGLLYLLLYGNSGWLGEWLYEHDLQIMFAWPGILLVTIFVTCSFVAR
ELIPLMQQQDASEEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQAEAAFASASLLALIALFTLMLKAVVEWRQQRSLSANDNKEQSPL

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=273, Percent_Identity=59.3406593406593, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1788764, Length=207, Percent_Identity=40.0966183574879, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1786980, Length=220, Percent_Identity=34.0909090909091, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32021; Mature: 32021

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFKPLRVGEAPLIKWSLITLAVFLGLLLLLLPLVSIFQQAFVGGWERYIGHLTQPDSL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCH
HAIGLTLMVAALTVPINLVFGIMLAWSVTRFEYPGRKLLITLIDIPFAVSPVVAGLLYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
LYGNSGWLGEWLYEHDLQIMFAWPGILLVTIFVTCSFVARELIPLMQQQDASEEEAAVIL
HHCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCEEEEEEEH
EDYQAEAAFASASLLALIALFTLMLKAVVEWRQQRSLSANDNKEQSPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSFKPLRVGEAPLIKWSLITLAVFLGLLLLLLPLVSIFQQAFVGGWERYIGHLTQPDSL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCH
HAIGLTLMVAALTVPINLVFGIMLAWSVTRFEYPGRKLLITLIDIPFAVSPVVAGLLYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
LYGNSGWLGEWLYEHDLQIMFAWPGILLVTIFVTCSFVARELIPLMQQQDASEEEAAVIL
HHCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCEEEEEEEH
EDYQAEAAFASASLLALIALFTLMLKAVVEWRQQRSLSANDNKEQSPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]