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The map label for this gene is epsE [H]

Identifier: 160873280

GI number: 160873280

Start: 183293

End: 184858

Strand: Direct

Name: epsE [H]

Synonym: Sbal195_0154

Alternate gene names: 160873280

Gene position: 183293-184858 (Clockwise)

Preceding gene: 160873279

Following gene: 160873281

Centisome position: 3.43

GC content: 51.28

Gene sequence:

>1566_bases
ATGAGTGAAATACAATTATCCCAAGTCGATGACCTCAGCCAAGTCTCCAATGAGCTAGGTCTGGAAGCTGAAGGTGATGA
GGTGTTTCGTTCGAGCAGTAAAGAGCGTTTACCCTTTGCCTTTGCCCATCGCCACGATGTGGTGCTTGCGCCGGGTGAAA
CGGGCGAGTTGAGCTTATTTTATACCAGCAAAACACCGTTAACTGCCATGCTCGAAGCACGGCGCTATTCGGGTGTCGAT
TTGCCTTTGGTTAAGCTCGAAGTCGCGAAGTTTGAGGCCAAACTCACTCAAGCGTATCAGGCGAACTCGTCTGAAGCGCA
GCAGTTGATGGAAGATATCGGCAACGAGATGGATTTATTCACCCTTGCCGAAGAGCTGCCGCAGACCGAAGACTTGCTCG
AAGGTGATGATGACGCGCCTATCATCAAACTGATTAATGCCTTGTTATCTGAAGCGATTAAAGAAGAAGCGTCGGATATC
CACATCGAAACCTACGAGAAACAGCTCGTAGTGCGTTTCCGTATCGATGGTGTGCTCAAGGAAGTCCTCAAGCCAAACCG
TAAGCTGTCGTCACTGTTAGTGTCGCGGATTAAGGTGATGGCGCGCCTCGATATCGCTGAAAAACGTGTGCCACAGGATG
GCCGTATTTCGCTGCGTATCGCAGGTCGTGCGGTGGATGTGCGGGTATCGACCATGCCATCGAGCCATGGCGAGCGCGTC
GTGCTGCGTTTGCTGGATAAAAATACCGGTAACCTCGATTTAGAACAATTGGGGATGACCGACGGTATTCGGGTTAAGTT
TGAAGATCTTATCCGTCGCCCCCACGGCATCATTCTGGTCACTGGCCCAACGGGTTCGGGTAAAAGTACCACCTTGTATG
CGGGTCTGACTGAGATCAACTCAAAAGACACCAACATTCTTACCGTGGAAGACCCAATCGAATACGAGCTAGACGGTATC
GGTCAAACCCAAGTTAACACTAAGGCTGACATGACCTTCGCCCGAGGTCTGCGCGCGATTTTGCGTCAAGATCCCGATGT
GGTGATGATAGGTGAAATCCGTGACTTGGAAACCGCACAAATTGCGGTGCAAGCATCACTCACTGGTCACATGGTAATTT
CAACCCTGCATACCAACACTGCGTCTGGCGCGATAACGCGTTTGCAAGACATGGGCGTTGAGCCATTCCTTGTATCATCG
AGTTTGCTGGGCGTGTTGGCGCAACGTTTGATCCGTACCCTTTGTCCAAAATGTAAAATCGAGCACGTGCCCGATGCGCG
TGAGCGTGAGTTACTGGGCATAGCGGCCGATGACGACAGACGTATTTTCCGTGCCAATGGCTGTAAATCCTGTGGTAACA
ATGGCTACCGTGGTCGAACGGGTATTCATGAGTTATTGCTGGTTGACGACAATGTCCGCGAATTGATCCATGGCGGCCGC
GGTGAGTTAGCTATCGAAAAGTATATCCGTCAGTTTGTGCCGAGCATTCGTCACGATGGCATGAGCAAAGTGCTCGCGGG
CATCACCACTCTCGAAGAAGTGCTACGTGTGACTCGCGAGGAGTAA

Upstream 100 bases:

>100_bases
TTAGAGCGTTATAAAGAAGGCAAAGGTTTAGATGCCCAGATGCGCCAAACGGATCCGACCTTAAAGTCACTGGAAGAAAA
CAAGAATAAAGATAAAACCA

Downstream 100 bases:

>100_bases
TTTATGCCAGCATTTGAATATAAGGCACTGGATGCCAAGGGAAAGCAGCTTAAGGGCGTAATCGAGGCGGATACCGCCAG
ACACGCCCGTAGCCAGTTGC

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSEIQLSQVDDLSQVSNELGLEAEGDEVFRSSSKERLPFAFAHRHDVVLAPGETGELSLFYTSKTPLTAMLEARRYSGVD
LPLVKLEVAKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDI
HIETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
VLRLLDKNTGNLDLEQLGMTDGIRVKFEDLIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELDGI
GQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLIRTLCPKCKIEHVPDARERELLGIAADDDRRIFRANGCKSCGNNGYRGRTGIHELLLVDDNVRELIHGGR
GELAIEKYIRQFVPSIRHDGMSKVLAGITTLEEVLRVTREE

Sequences:

>Translated_521_residues
MSEIQLSQVDDLSQVSNELGLEAEGDEVFRSSSKERLPFAFAHRHDVVLAPGETGELSLFYTSKTPLTAMLEARRYSGVD
LPLVKLEVAKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDI
HIETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
VLRLLDKNTGNLDLEQLGMTDGIRVKFEDLIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELDGI
GQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLIRTLCPKCKIEHVPDARERELLGIAADDDRRIFRANGCKSCGNNGYRGRTGIHELLLVDDNVRELIHGGR
GELAIEKYIRQFVPSIRHDGMSKVLAGITTLEEVLRVTREE
>Mature_520_residues
SEIQLSQVDDLSQVSNELGLEAEGDEVFRSSSKERLPFAFAHRHDVVLAPGETGELSLFYTSKTPLTAMLEARRYSGVDL
PLVKLEVAKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIH
IETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERVV
LRLLDKNTGNLDLEQLGMTDGIRVKFEDLIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELDGIG
QTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSS
LLGVLAQRLIRTLCPKCKIEHVPDARERELLGIAADDDRRIFRANGCKSCGNNGYRGRTGIHELLLVDDNVRELIHGGRG
ELAIEKYIRQFVPSIRHDGMSKVLAGITTLEEVLRVTREE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=57.2025052192067, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=43.2291666666667, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI87082188, Length=245, Percent_Identity=36.734693877551, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57691; Mature: 57560

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIQLSQVDDLSQVSNELGLEAEGDEVFRSSSKERLPFAFAHRHDVVLAPGETGELSLF
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEECCCCEEECCCCCCCEEEE
YTSKTPLTAMLEARRYSGVDLPLVKLEVAKFEAKLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLVVRFRIDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCCEE
VLRLLDKNTGNLDLEQLGMTDGIRVKFEDLIRRPHGIILVTGPTGSGKSTTLYAGLTEIN
EHEEECCCCCCCCHHHCCCCCCCEEEHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHHCC
SKDTNILTVEDPIEYELDGIGQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHE
IAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPKCKI
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHVPDARERELLGIAADDDRRIFRANGCKSCGNNGYRGRTGIHELLLVDDNVRELIHGGR
CCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCC
GELAIEKYIRQFVPSIRHDGMSKVLAGITTLEEVLRVTREE
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIQLSQVDDLSQVSNELGLEAEGDEVFRSSSKERLPFAFAHRHDVVLAPGETGELSLF
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEECCCCEEECCCCCCCEEEE
YTSKTPLTAMLEARRYSGVDLPLVKLEVAKFEAKLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLVVRFRIDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCCEE
VLRLLDKNTGNLDLEQLGMTDGIRVKFEDLIRRPHGIILVTGPTGSGKSTTLYAGLTEIN
EHEEECCCCCCCCHHHCCCCCCCEEEHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHHCC
SKDTNILTVEDPIEYELDGIGQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHE
IAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPKCKI
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHVPDARERELLGIAADDDRRIFRANGCKSCGNNGYRGRTGIHELLLVDDNVRELIHGGR
CCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCC
GELAIEKYIRQFVPSIRHDGMSKVLAGITTLEEVLRVTREE
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]