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The map label for this gene is rlmF [H]

Identifier: 160873258

GI number: 160873258

Start: 154658

End: 155755

Strand: Direct

Name: rlmF [H]

Synonym: Sbal195_0132

Alternate gene names: 160873258

Gene position: 154658-155755 (Clockwise)

Preceding gene: 160873257

Following gene: 160873263

Centisome position: 2.89

GC content: 51.18

Gene sequence:

>1098_bases
ATGTCAAAACCTGCCGTGAAATCCGTTCCATCAGCGACTGCGAAAACCGCAACCCGCGCAGCGAACCCCCGCCAAAAAGC
TAAAGCACCCAAGCAGGCGAAACCTGAAGGAAAAGGCAGAGCAAAGCCAAGCAAAGACAAACCCAGAGCTGAAATCAAAA
AAGCCCTGCATCCGCGTAACGCCCACTTGAATGGCTATGATTTTCCGGCGCTAATCAGTGCATTTCCTCGGCTGAAAACC
TTTGTGCGGCCCACGCCCTATGGCGCGCTATCGGTAAATTTTGCCGACCCCTTGGCAGTTAAAACCCTCAATGCGGCGCT
ACTCAAACATCACTATGGAATTGGTTCTTGGAATATTCCTGAAGGAGCGTTATGTCCTCCTATTCCCGGTCGCGTCGACT
ATGTGCATTACGTGGCAGATCTGTTAGCCGAAGGCGATAAGTCATGTGCCATGGATAAGGCGAGGGTGCTGGATATTGGC
ACGGGCGCGAACGGCATTTATCCCATATTGGGCTGCCAAGTGTACGGCTGGCAATATGTTGCCTCGGATATCAATGCCCA
TTCACTCGCCAATGTGCAGAGTATTATCGAGCAAAACCCCGTGCTGCAAGGGCGGATAAGCTTACGATTACAGCCGGATG
ACAAGGCGGTCTTCAAGGGAATTATTCAAGCCGAAGAACGTTTTGAGTTAACCCTGTGTAATCCTCCGTTTCATGCCTCT
ATGGCCGAGGCGAGCGAAGGTACAAAGCGTAAGGTGAATAATCTACAGCTTAATCGGGGGAGCTCAGTAAAGGCTGCACC
TAAGCTGAATTTCGGCGGCCAAGCGGCAGAGCTTTGGTGCCAAGGTGGAGAGGGACAGTTCTTGGCGACCATGATCCGTG
AGAGTCAGATGTTTGCAGACCAATGCCTGTGGTTCACTAGCCTAGTGTCGAAGCAAGAAAACCTGAAACCTTGTTATCAA
GCCTTAGCACAGTTGAACGTCGATACAGTGAAAACGATTGAAATGCAGCAAGGCAATAAAATTACGCGGGTCTTAGCGTG
GAGCTTCCAGTCGGCCGCTAAGCGCAAACTCTGGCGCGCTGAACACTTAGCCCGTTAA

Upstream 100 bases:

>100_bases
CCTTGATTGTAAACTTACTCTTTACCTCTTATCTGTACGATTAATTCATTGTCTTATTTTATGGGCTGACTGATGTCGGC
CCAGTGTTACAAGAGCTGCT

Downstream 100 bases:

>100_bases
GATTTTCAGTTTACTTAATAAAAAAACAGGCGCCATTATTGGCGCCTGTTTTTTTAAAGGTAAAAGAACCACTGTTACTG
CGGCTTTTTTTCCTGATGAT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSKPAVKSVPSATAKTATRAANPRQKAKAPKQAKPEGKGRAKPSKDKPRAEIKKALHPRNAHLNGYDFPALISAFPRLKT
FVRPTPYGALSVNFADPLAVKTLNAALLKHHYGIGSWNIPEGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIG
TGANGIYPILGCQVYGWQYVASDINAHSLANVQSIIEQNPVLQGRISLRLQPDDKAVFKGIIQAEERFELTLCNPPFHAS
MAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGEGQFLATMIRESQMFADQCLWFTSLVSKQENLKPCYQ
ALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKLWRAEHLAR

Sequences:

>Translated_365_residues
MSKPAVKSVPSATAKTATRAANPRQKAKAPKQAKPEGKGRAKPSKDKPRAEIKKALHPRNAHLNGYDFPALISAFPRLKT
FVRPTPYGALSVNFADPLAVKTLNAALLKHHYGIGSWNIPEGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIG
TGANGIYPILGCQVYGWQYVASDINAHSLANVQSIIEQNPVLQGRISLRLQPDDKAVFKGIIQAEERFELTLCNPPFHAS
MAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGEGQFLATMIRESQMFADQCLWFTSLVSKQENLKPCYQ
ALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKLWRAEHLAR
>Mature_364_residues
SKPAVKSVPSATAKTATRAANPRQKAKAPKQAKPEGKGRAKPSKDKPRAEIKKALHPRNAHLNGYDFPALISAFPRLKTF
VRPTPYGALSVNFADPLAVKTLNAALLKHHYGIGSWNIPEGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIGT
GANGIYPILGCQVYGWQYVASDINAHSLANVQSIIEQNPVLQGRISLRLQPDDKAVFKGIIQAEERFELTLCNPPFHASM
AEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGEGQFLATMIRESQMFADQCLWFTSLVSKQENLKPCYQA
LAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKLWRAEHLAR

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=300, Percent_Identity=31.3333333333333, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI87081786, Length=304, Percent_Identity=49.0131578947368, Blast_Score=311, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI32564931, Length=310, Percent_Identity=26.1290322580645, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=309, Percent_Identity=24.2718446601942, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281363415, Length=300, Percent_Identity=27, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922302, Length=300, Percent_Identity=27, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 40021; Mature: 39890

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPAVKSVPSATAKTATRAANPRQKAKAPKQAKPEGKGRAKPSKDKPRAEIKKALHPRN
CCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
AHLNGYDFPALISAFPRLKTFVRPTPYGALSVNFADPLAVKTLNAALLKHHYGIGSWNIP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
EGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIGTGANGIYPILGCQVYGWQYV
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCHHHCEEEECHHHH
ASDINAHSLANVQSIIEQNPVLQGRISLRLQPDDKAVFKGIIQAEERFELTLCNPPFHAS
HHCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCH
MAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGEGQFLATMIRESQMFAD
HHHHCCCHHHHHCCEEECCCCCCCCCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHHH
QCLWFTSLVSKQENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKLWRA
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEECCCCCCEEHHHHHHHHHHHHHHHHHH
EHLAR
HHHCC
>Mature Secondary Structure 
SKPAVKSVPSATAKTATRAANPRQKAKAPKQAKPEGKGRAKPSKDKPRAEIKKALHPRN
CCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
AHLNGYDFPALISAFPRLKTFVRPTPYGALSVNFADPLAVKTLNAALLKHHYGIGSWNIP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
EGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIGTGANGIYPILGCQVYGWQYV
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCHHHCEEEECHHHH
ASDINAHSLANVQSIIEQNPVLQGRISLRLQPDDKAVFKGIIQAEERFELTLCNPPFHAS
HHCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCH
MAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGEGQFLATMIRESQMFAD
HHHHCCCHHHHHCCEEECCCCCCCCCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHHH
QCLWFTSLVSKQENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKLWRA
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEECCCCCCEEHHHHHHHHHHHHHHHHHH
EHLAR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA