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The map label for this gene is fdnI [H]

Identifier: 160873233

GI number: 160873233

Start: 128796

End: 129437

Strand: Direct

Name: fdnI [H]

Synonym: Sbal195_0107

Alternate gene names: 160873233

Gene position: 128796-129437 (Clockwise)

Preceding gene: 160873232

Following gene: 160873234

Centisome position: 2.41

GC content: 49.07

Gene sequence:

>642_bases
ATGAGCAAGCATGATAAGCAAGAAATGATAGTGCGTCACAAGCTGTTCGATCGCATCTGTCATTGGTTCATTGTAGCAGT
TGGCCTAGTGACCTTTCTCACTGGCTTTTCCTTCTTCTATCCGTCTTTTCAATGGTTGGGGGCGATAGCGGGTACTCCGC
AATTGGCGAAGTTTATTCACCCCATTGCGGGCTTGATGATGTGCTTACCGCTGATGTTGATGTTGGTGCGTTACTACAAA
CACAACAAATGGGAAAAACACGATCTCGCTTGGATGCTCGCCATCAAGGATGTGATGTTCGAGAACGAGGAGAAAATCCC
GCCTATCGGCCATTACAACCCAGGGCAAAAGGTATTGTTTCGCGCCTTCGTGCTGACGTCTGTCACTTTAACCGTGACGG
GGATCATCATGTGGCAACCGTATTTTGCGCCCTATTTCTCTGCCACTGTGGTGGGGTGGGCCATTCTGTTGCACGCCATT
TGTGCGCTCATCATGTTGATTTTTGTGATGGTACATTTCTGGATGGCGACGTGGGTGGAAGGGTCGGTAACGGGCATGCT
TTACGGCAAGGTCTCTAAGGCTTGGTGCCGTAAGCATCACCCTGTGATGTTGGATGAACATTCGGACGTGGAGAAAAAAT
AA

Upstream 100 bases:

>100_bases
GTCTGCATAAGATTACCGTCGGTCGCAACATAGTGGAAGAGGACCAGCCGGGTTATCAACCGCCAGAGGAAGAGGTTAAG
CAGGAAGAGGAGGGCGACAA

Downstream 100 bases:

>100_bases
TGAGTCATACAGCCGAGATACCTCTGGTCCCCGGCAGCGAATCGCCGCTGGAACTAAAACCGTTAAAAGCGGCAGATCCC
CAAATTGTGTATCACAGAAG

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSKHDKQEMIVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIHPIAGLMMCLPLMLMLVRYYK
HNKWEKHDLAWMLAIKDVMFENEEKIPPIGHYNPGQKVLFRAFVLTSVTLTVTGIIMWQPYFAPYFSATVVGWAILLHAI
CALIMLIFVMVHFWMATWVEGSVTGMLYGKVSKAWCRKHHPVMLDEHSDVEKK

Sequences:

>Translated_213_residues
MSKHDKQEMIVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIHPIAGLMMCLPLMLMLVRYYK
HNKWEKHDLAWMLAIKDVMFENEEKIPPIGHYNPGQKVLFRAFVLTSVTLTVTGIIMWQPYFAPYFSATVVGWAILLHAI
CALIMLIFVMVHFWMATWVEGSVTGMLYGKVSKAWCRKHHPVMLDEHSDVEKK
>Mature_212_residues
SKHDKQEMIVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIHPIAGLMMCLPLMLMLVRYYKH
NKWEKHDLAWMLAIKDVMFENEEKIPPIGHYNPGQKVLFRAFVLTSVTLTVTGIIMWQPYFAPYFSATVVGWAILLHAIC
ALIMLIFVMVHFWMATWVEGSVTGMLYGKVSKAWCRKHHPVMLDEHSDVEKK

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=209, Percent_Identity=43.0622009569378, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1790325, Length=210, Percent_Identity=38.5714285714286, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24736; Mature: 24605

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHDKQEMIVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PIAGLMMCLPLMLMLVRYYKHNKWEKHDLAWMLAIKDVMFENEEKIPPIGHYNPGQKVLF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
RAFVLTSVTLTVTGIIMWQPYFAPYFSATVVGWAILLHAICALIMLIFVMVHFWMATWVE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSVTGMLYGKVSKAWCRKHHPVMLDEHSDVEKK
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure 
SKHDKQEMIVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PIAGLMMCLPLMLMLVRYYKHNKWEKHDLAWMLAIKDVMFENEEKIPPIGHYNPGQKVLF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
RAFVLTSVTLTVTGIIMWQPYFAPYFSATVVGWAILLHAICALIMLIFVMVHFWMATWVE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSVTGMLYGKVSKAWCRKHHPVMLDEHSDVEKK
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]