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The map label for this gene is fdoH [H]

Identifier: 160873232

GI number: 160873232

Start: 127888

End: 128799

Strand: Direct

Name: fdoH [H]

Synonym: Sbal195_0106

Alternate gene names: 160873232

Gene position: 127888-128799 (Clockwise)

Preceding gene: 160873231

Following gene: 160873233

Centisome position: 2.39

GC content: 53.18

Gene sequence:

>912_bases
ATGGCATCACAAGACATTATCCGCCGCTCCGGTACTTCAGCGGTGACGCCTGCGGCAAAGGGGCGTAATTCGTCAGAAAA
GGTCGCCAAGCTGTTCGATGCCACTAAGTGTAATGGCTGTAAAGCCTGCCAAGTGGCGTGTTCTGAGTGGAATGATTTAC
GGGAAGAAGTGGGCACGTTCCAAGGTAGTTACCAAAACCCTATGTCGCTATCGGGCCAATGTTGGACCCTGATGGAGTTC
CATGAGGTGATGGAAAGCAACAAGCTGCGTTGGCAATTTACCCACTCGGCCTGTATGCACTGTGCCGACCCTGCCTGCTT
AACTGCCTGTTCGACTTCGGGCGCGATTATCCAGCACGCCAATGGTGTGGTGGACTTCGATTCGGACAAGTGTATCGGCT
GCGGTTATTGCGCCAGTGCTTGTCCCTTCGATGTGCCCAAAATCGATCCTAAGGACAACAAGGCCTACAAGTGCACTATG
TGCTCTGACCGTTTGGCCGTGGGATTGGAACCTTCCTGTGTTAAGTCCTGTACCACAGGGGCGCTGCGTTTTGGCACCCG
TGAGGACATGTTGTTTTACTCGCAAAAACGGGTCGAAGAACTGAAGGAAAGGGGCTTCGCTAAGGCAGGACTCTATAACC
CTGAGGGTGTAGGTGGTACGGGGATGATGATGATATTGCACGATATCAATCAGCCCGAAGGCTACAACATGCCGGATAAC
CCGCAGATCCCGCTGTCACTGACTCTGTGGCAAGACTGGGTCAAACCTTTAGGCACTATGGGCATTCTTGCCACTGCCGC
TGTCGCTTGTCTGCATAAGATTACCGTCGGTCGCAACATAGTGGAAGAGGACCAGCCGGGTTATCAACCGCCAGAGGAAG
AGGTTAAGCAGGAAGAGGAGGGCGACAAATGA

Upstream 100 bases:

>100_bases
CCTGTAACGTACTCACCACGGAAGTGGGGGATGCAAATACACAGGTGCCTGAGTTCAAAGCATTTTTGGTTAACATCACT
AAAGCGGAGGGGATCTGATT

Downstream 100 bases:

>100_bases
GCAAGCATGATAAGCAAGAAATGATAGTGCGTCACAAGCTGTTCGATCGCATCTGTCATTGGTTCATTGTAGCAGTTGGC
CTAGTGACCTTTCTCACTGG

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MASQDIIRRSGTSAVTPAAKGRNSSEKVAKLFDATKCNGCKACQVACSEWNDLREEVGTFQGSYQNPMSLSGQCWTLMEF
HEVMESNKLRWQFTHSACMHCADPACLTACSTSGAIIQHANGVVDFDSDKCIGCGYCASACPFDVPKIDPKDNKAYKCTM
CSDRLAVGLEPSCVKSCTTGALRFGTREDMLFYSQKRVEELKERGFAKAGLYNPEGVGGTGMMMILHDINQPEGYNMPDN
PQIPLSLTLWQDWVKPLGTMGILATAAVACLHKITVGRNIVEEDQPGYQPPEEEVKQEEEGDK

Sequences:

>Translated_303_residues
MASQDIIRRSGTSAVTPAAKGRNSSEKVAKLFDATKCNGCKACQVACSEWNDLREEVGTFQGSYQNPMSLSGQCWTLMEF
HEVMESNKLRWQFTHSACMHCADPACLTACSTSGAIIQHANGVVDFDSDKCIGCGYCASACPFDVPKIDPKDNKAYKCTM
CSDRLAVGLEPSCVKSCTTGALRFGTREDMLFYSQKRVEELKERGFAKAGLYNPEGVGGTGMMMILHDINQPEGYNMPDN
PQIPLSLTLWQDWVKPLGTMGILATAAVACLHKITVGRNIVEEDQPGYQPPEEEVKQEEEGDK
>Mature_302_residues
ASQDIIRRSGTSAVTPAAKGRNSSEKVAKLFDATKCNGCKACQVACSEWNDLREEVGTFQGSYQNPMSLSGQCWTLMEFH
EVMESNKLRWQFTHSACMHCADPACLTACSTSGAIIQHANGVVDFDSDKCIGCGYCASACPFDVPKIDPKDNKAYKCTMC
SDRLAVGLEPSCVKSCTTGALRFGTREDMLFYSQKRVEELKERGFAKAGLYNPEGVGGTGMMMILHDINQPEGYNMPDNP
QIPLSLTLWQDWVKPLGTMGILATAAVACLHKITVGRNIVEEDQPGYQPPEEEVKQEEEGDK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=296, Percent_Identity=51.6891891891892, Blast_Score=340, Evalue=5e-95,
Organism=Escherichia coli, GI1787749, Length=291, Percent_Identity=51.5463917525773, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1787122, Length=160, Percent_Identity=37.5, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=34.2391304347826, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787872, Length=160, Percent_Identity=37.5, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI226510944, Length=216, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=181, Percent_Identity=32.5966850828729, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787963, Length=181, Percent_Identity=24.3093922651934, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=30.6748466257669, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=103, Percent_Identity=32.0388349514563, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788811, Length=163, Percent_Identity=26.3803680981595, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=32.183908045977, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33194; Mature: 33063

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
6.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQDIIRRSGTSAVTPAAKGRNSSEKVAKLFDATKCNGCKACQVACSEWNDLREEVGTF
CCCHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QGSYQNPMSLSGQCWTLMEFHEVMESNKLRWQFTHSACMHCADPACLTACSTSGAIIQHA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHHHHHHCCCCCEEECC
NGVVDFDSDKCIGCGYCASACPFDVPKIDPKDNKAYKCTMCSDRLAVGLEPSCVKSCTTG
CCEEECCCCCCEECCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
ALRFGTREDMLFYSQKRVEELKERGFAKAGLYNPEGVGGTGMMMILHDINQPEGYNMPDN
HHHCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCC
PQIPLSLTLWQDWVKPLGTMGILATAAVACLHKITVGRNIVEEDQPGYQPPEEEVKQEEE
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHC
GDK
CCC
>Mature Secondary Structure 
ASQDIIRRSGTSAVTPAAKGRNSSEKVAKLFDATKCNGCKACQVACSEWNDLREEVGTF
CCHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QGSYQNPMSLSGQCWTLMEFHEVMESNKLRWQFTHSACMHCADPACLTACSTSGAIIQHA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHHHHHHCCCCCEEECC
NGVVDFDSDKCIGCGYCASACPFDVPKIDPKDNKAYKCTMCSDRLAVGLEPSCVKSCTTG
CCEEECCCCCCEECCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
ALRFGTREDMLFYSQKRVEELKERGFAKAGLYNPEGVGGTGMMMILHDINQPEGYNMPDN
HHHCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCC
PQIPLSLTLWQDWVKPLGTMGILATAAVACLHKITVGRNIVEEDQPGYQPPEEEVKQEEE
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHC
GDK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]