Click here to switch to the map view.

The map label for this gene is tag [H]

Identifier: 160873140

GI number: 160873140

Start: 15445

End: 16047

Strand: Direct

Name: tag [H]

Synonym: Sbal195_0014

Alternate gene names: 160873140

Gene position: 15445-16047 (Clockwise)

Preceding gene: 160873137

Following gene: 160873147

Centisome position: 0.29

GC content: 46.1

Gene sequence:

>603_bases
ATGGAAGAGATCCGTTGTGGCTGGGTAAGTGATGATCCCTTGTATCGTGAGTATCATGACAAAGTGTGGGGGCGGCCGGT
TTATGATCCTAAGGAGCTGTTTGCTAAGTTGTGTTTAGACGGTCAACAGGCCGGATTATCGTGGATTACGATCCTGAAGA
AACAACAAAACTATGAGCAGGCCTTTGCCAATTTTGAACCAGAAGTGATTGCTGGCTTTGATGAGGCGAAAGTGGAGGAA
CTTATGTTGAATCCCGGCATAGTGCGCAATCGCTTGAAGGTGAGCTCTATCATCAAGAATGCCAAAGGATATTTGGCTTA
TACTGCCGATGGGAAGGATTTCTCTGCATTCTTATGGAGCTTTGTTGGGGGTAAGCCCATAGTTAATCAGTTTACTGCTA
TGTCGCAGGTTCCAGCGCAAACGCCAGAATCTGAAGCCATGTCAAAAGCGTTAAAGAAGCTTGGATTTAATTTCGTCGGC
CCAACAATTTGTTATGCCTTTATGCAGGCGGTGGGTATGGTGAATGACCACCTTGTAGAGTGTATTTCCTACCAGGCCTG
TGTTGATTCCTGTGCCGGTCAGCATAACTGCGAACATCAGTAA

Upstream 100 bases:

>100_bases
CGTCAGCCTGAATAGAAAATGGGTTCGATTATACCTTGTAGATATCTGCTTATGTAGGTTATTTTTTACCTTCTTAATTA
AAACAATAAGGTTATATACC

Downstream 100 bases:

>100_bases
TCGCTCATCTTGCTGATGTGATGTAGGACAGTACTCATAGCAATATTCGAGTTCTTGTCCATATACACAATAACAATGCA
CAAAATGTAAAAAAGCATGT

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MEEIRCGWVSDDPLYREYHDKVWGRPVYDPKELFAKLCLDGQQAGLSWITILKKQQNYEQAFANFEPEVIAGFDEAKVEE
LMLNPGIVRNRLKVSSIIKNAKGYLAYTADGKDFSAFLWSFVGGKPIVNQFTAMSQVPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHLVECISYQACVDSCAGQHNCEHQ

Sequences:

>Translated_200_residues
MEEIRCGWVSDDPLYREYHDKVWGRPVYDPKELFAKLCLDGQQAGLSWITILKKQQNYEQAFANFEPEVIAGFDEAKVEE
LMLNPGIVRNRLKVSSIIKNAKGYLAYTADGKDFSAFLWSFVGGKPIVNQFTAMSQVPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHLVECISYQACVDSCAGQHNCEHQ
>Mature_200_residues
MEEIRCGWVSDDPLYREYHDKVWGRPVYDPKELFAKLCLDGQQAGLSWITILKKQQNYEQAFANFEPEVIAGFDEAKVEE
LMLNPGIVRNRLKVSSIIKNAKGYLAYTADGKDFSAFLWSFVGGKPIVNQFTAMSQVPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHLVECISYQACVDSCAGQHNCEHQ

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=180, Percent_Identity=55, Blast_Score=216, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 22462; Mature: 22462

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIRCGWVSDDPLYREYHDKVWGRPVYDPKELFAKLCLDGQQAGLSWITILKKQQNYEQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AFANFEPEVIAGFDEAKVEELMLNPGIVRNRLKVSSIIKNAKGYLAYTADGKDFSAFLWS
HHHCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
FVGGKPIVNQFTAMSQVPAQTPESEAMSKALKKLGFNFVGPTICYAFMQAVGMVNDHLVE
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CISYQACVDSCAGQHNCEHQ
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEEIRCGWVSDDPLYREYHDKVWGRPVYDPKELFAKLCLDGQQAGLSWITILKKQQNYEQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AFANFEPEVIAGFDEAKVEELMLNPGIVRNRLKVSSIIKNAKGYLAYTADGKDFSAFLWS
HHHCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
FVGGKPIVNQFTAMSQVPAQTPESEAMSKALKKLGFNFVGPTICYAFMQAVGMVNDHLVE
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CISYQACVDSCAGQHNCEHQ
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]