Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 159900945

GI number: 159900945

Start: 5672240

End: 5672875

Strand: Direct

Name: def [H]

Synonym: Haur_4432

Alternate gene names: 159900945

Gene position: 5672240-5672875 (Clockwise)

Preceding gene: 159900940

Following gene: 159900946

Centisome position: 89.37

GC content: 49.21

Gene sequence:

>636_bases
ATGGCAGTACGTCGGGTATTGCAAATAGAAGATCCAACTGATGCAGTGGTATTACGGGCCAAGGCCAAACGCATCACCAG
TTTTGATCAAAGTTTGGCTGGTTTAGTTGATGATATGATTGAAACCATGCGCGAAGCTCGGGGTGTTGGCATTGCCGCGC
CGCAAGTTGGGGTTTCACGGCGCGTAGTGGTGATTGAAGAGCCAGCCCAATACGAAGAACATGAAGATGGTACGCAGACC
CAAATTGCTCCCGCCGTGCTGTATGTGATGGTCAACCCAGAGATTATCAAAGCCAGCGAAGAAACCCATATGCTGCAAGA
AGGCTGTTTGAGCCTGCCTGGCCGCTATACCAAAGTGCCGCGCAATAAATGGGTAACGATCAAATATTACGATTTGAAGG
GTCGCGAACAGCGCTTGCGCCATATTCCTGCTGAAGATTACAAAGTTGGGCACATTGCTCAGCACGAGTTGGATCACCTT
GATGGGATTATGTTTACCGACCGCATGACCGAAGAAAGCAAATTGGTCGATTATCGCAAAGAAAGTGAAAGCGCACGCCT
CAAACGGCGCGGTTTGTTGGCGCGTAAAAAACGCCCCGATGCTGAACTCGAATCAGAATCGACAACCAATGAATAA

Upstream 100 bases:

>100_bases
TACGTAAGCCATGTCAAGCCCAACTCATCAGTATGTAAAGATCAGATTAAGAAATGAGGGTGCTATAATGAACGCTAGCA
ATAATCAAGGAGGTTTCCCC

Downstream 100 bases:

>100_bases
ATGTAATGGTTAATCGATGACAGCCCCTCACCCCCTAGCCCCCTAGCCCGCAGGCGAGGCGAGGGGGAACCGAGTTACAA
GTGCTCCCCTCTCCCGCCGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAVRRVLQIEDPTDAVVLRAKAKRITSFDQSLAGLVDDMIETMREARGVGIAAPQVGVSRRVVVIEEPAQYEEHEDGTQT
QIAPAVLYVMVNPEIIKASEETHMLQEGCLSLPGRYTKVPRNKWVTIKYYDLKGREQRLRHIPAEDYKVGHIAQHELDHL
DGIMFTDRMTEESKLVDYRKESESARLKRRGLLARKKRPDAELESESTTNE

Sequences:

>Translated_211_residues
MAVRRVLQIEDPTDAVVLRAKAKRITSFDQSLAGLVDDMIETMREARGVGIAAPQVGVSRRVVVIEEPAQYEEHEDGTQT
QIAPAVLYVMVNPEIIKASEETHMLQEGCLSLPGRYTKVPRNKWVTIKYYDLKGREQRLRHIPAEDYKVGHIAQHELDHL
DGIMFTDRMTEESKLVDYRKESESARLKRRGLLARKKRPDAELESESTTNE
>Mature_210_residues
AVRRVLQIEDPTDAVVLRAKAKRITSFDQSLAGLVDDMIETMREARGVGIAAPQVGVSRRVVVIEEPAQYEEHEDGTQTQ
IAPAVLYVMVNPEIIKASEETHMLQEGCLSLPGRYTKVPRNKWVTIKYYDLKGREQRLRHIPAEDYKVGHIAQHELDHLD
GIMFTDRMTEESKLVDYRKESESARLKRRGLLARKKRPDAELESESTTNE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=34.7058823529412, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=34.1176470588235, Blast_Score=87, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24645728, Length=179, Percent_Identity=31.8435754189944, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=32.1428571428571, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 24097; Mature: 23966

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRRVLQIEDPTDAVVLRAKAKRITSFDQSLAGLVDDMIETMREARGVGIAAPQVGVSR
CCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
RVVVIEEPAQYEEHEDGTQTQIAPAVLYVMVNPEIIKASEETHMLQEGCLSLPGRYTKVP
EEEEEECCCCCCCCCCCCHHHHHEEEEEEEECCHHEECCHHHHHHHHHHHCCCCCCEECC
RNKWVTIKYYDLKGREQRLRHIPAEDYKVGHIAQHELDHLDGIMFTDRMTEESKLVDYRK
CCCEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHH
ESESARLKRRGLLARKKRPDAELESESTTNE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVRRVLQIEDPTDAVVLRAKAKRITSFDQSLAGLVDDMIETMREARGVGIAAPQVGVSR
CCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
RVVVIEEPAQYEEHEDGTQTQIAPAVLYVMVNPEIIKASEETHMLQEGCLSLPGRYTKVP
EEEEEECCCCCCCCCCCCHHHHHEEEEEEEECCHHEECCHHHHHHHHHHHCCCCCCEECC
RNKWVTIKYYDLKGREQRLRHIPAEDYKVGHIAQHELDHLDGIMFTDRMTEESKLVDYRK
CCCEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHH
ESESARLKRRGLLARKKRPDAELESESTTNE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA