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The map label for this gene is def [H]

Identifier: 159899991

GI number: 159899991

Start: 4377412

End: 4377939

Strand: Direct

Name: def [H]

Synonym: Haur_3474

Alternate gene names: 159899991

Gene position: 4377412-4377939 (Clockwise)

Preceding gene: 159899990

Following gene: 159899996

Centisome position: 68.97

GC content: 52.46

Gene sequence:

>528_bases
ATGGCGGTTGTACCACTGATCGAGCTTGGAAATCCGTTGTTACGCCAGCCAGCAACTCCATTTGCCGACCCAACCAGCCC
CGAAGTGGCCCGCATTTTGAACGACATGCGCGATACCTTGGCCGATATGCGACAACGAATTGGCTATGGCCGAGGTATTG
CCGCACCGCAAATTGGCGTGCTCAAGCGCTTGATTTTGATTGATACCCCCGACACCAATTTAGTGCTAGTCAACCCGCGT
TTTGAGCGCTGGAGCCGCGAGGAAGACGAACGCTATGAATCATGTTTTAGCTTTCCTGGCATTTGGGGTTTGGTGCAACG
CCCACTTGGTGTAACTGTGGTCGCCTATACGTTGGCGGGCGAGGAGCAACGCATCGAAGCGAGTGGCAGCCTCTCACGCA
TTATTCAGCACGAAATGGATCATCTTGATGGCTTTGTTTGGCTTGATCGTGGCCCTGATTTGCACAGCCTCTGCACCACC
CAAGAATATGAAAAACGCTATATTAGCCGCGAACGCGAATCACATTAA

Upstream 100 bases:

>100_bases
GCCTATCCCTTGGTGTTTGCGGTGGTTGGGGGCTTATTACTGTTTCAAGGCTGGGCGTTTAGCCGTTTCAAACTTTTTTA
ACCGAATCAAGGAGTCTGTT

Downstream 100 bases:

>100_bases
TACTGTGAGCATGGTTTGGGCTGCTTGAGCCGCTAGTTGCGCATGGCGATCGTCGATTCCATAGACCGCAATGTAAATCG
TAACCTCGGCGTTGGTCGTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MAVVPLIELGNPLLRQPATPFADPTSPEVARILNDMRDTLADMRQRIGYGRGIAAPQIGVLKRLILIDTPDTNLVLVNPR
FERWSREEDERYESCFSFPGIWGLVQRPLGVTVVAYTLAGEEQRIEASGSLSRIIQHEMDHLDGFVWLDRGPDLHSLCTT
QEYEKRYISRERESH

Sequences:

>Translated_175_residues
MAVVPLIELGNPLLRQPATPFADPTSPEVARILNDMRDTLADMRQRIGYGRGIAAPQIGVLKRLILIDTPDTNLVLVNPR
FERWSREEDERYESCFSFPGIWGLVQRPLGVTVVAYTLAGEEQRIEASGSLSRIIQHEMDHLDGFVWLDRGPDLHSLCTT
QEYEKRYISRERESH
>Mature_174_residues
AVVPLIELGNPLLRQPATPFADPTSPEVARILNDMRDTLADMRQRIGYGRGIAAPQIGVLKRLILIDTPDTNLVLVNPRF
ERWSREEDERYESCFSFPGIWGLVQRPLGVTVVAYTLAGEEQRIEASGSLSRIIQHEMDHLDGFVWLDRGPDLHSLCTTQ
EYEKRYISRERESH

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=33.9285714285714, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19972; Mature: 19841

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVPLIELGNPLLRQPATPFADPTSPEVARILNDMRDTLADMRQRIGYGRGIAAPQIGV
CCCCCHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LKRLILIDTPDTNLVLVNPRFERWSREEDERYESCFSFPGIWGLVQRPLGVTVVAYTLAG
HHHHHEEECCCCCEEEECCCHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCC
EEQRIEASGSLSRIIQHEMDHLDGFVWLDRGPDLHSLCTTQEYEKRYISRERESH
CHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVVPLIELGNPLLRQPATPFADPTSPEVARILNDMRDTLADMRQRIGYGRGIAAPQIGV
CCCCHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LKRLILIDTPDTNLVLVNPRFERWSREEDERYESCFSFPGIWGLVQRPLGVTVVAYTLAG
HHHHHEEECCCCCEEEECCCHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCC
EEQRIEASGSLSRIIQHEMDHLDGFVWLDRGPDLHSLCTTQEYEKRYISRERESH
CHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA