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The map label for this gene is psuK [H]

Identifier: 159899984

GI number: 159899984

Start: 4365876

End: 4366814

Strand: Direct

Name: psuK [H]

Synonym: Haur_3467

Alternate gene names: 159899984

Gene position: 4365876-4366814 (Clockwise)

Preceding gene: 159899983

Following gene: 159899985

Centisome position: 68.79

GC content: 52.29

Gene sequence:

>939_bases
ATGGAACATTTGGATACAGATTGGCGCGTGGTGGTTATTGGTGGCGCAGGGCTGGATATCAAAGGGCGTTTGCTTGAGCC
ATTGATTGAAAAAACCTCGAATCCAGGCCGTTTAACAATTAGTGTTGGCGGAGTTGCCCGCAACATTGCTGAAAATTTGG
CTCGCTTGGGCGCACAAAGCAGCCTGATTGCCGCGGTAGGCAACGATGAATTTGGGCGCTTAATCATCCAACAAACTGAA
GATGCTGGTGTTGATACCGATGCCATGATGATTTTAGAGGATCAACATTCGGCAGCCTATTTGGCGCTGCTCGATCGCAA
TGGCTTGTTGTATACGGCGCTCGATGATAGCCACTGTGCGCGGGCATTAACGCCCGATTATATTTACGATAACGTGCAGC
TATTGCGGGCAGCCGATGCAGTCTTTATTGATGCCAATTTGGCACGGGCAACTGCCGATGTGATTTTGCAAATTTGTGCC
GAAGAAGATATTCCAGTCTGTTTCGAGCCTGTGGCCTATGGTTTAGCAGGTCGCTACCGTGATCGTTTGCGTGGGATGTA
TTTGATTACGCCCAACGAGCGTGAGGCCGAAGCATTAACTGGCTTGCCGGTGACCTCGCCTGCCGAGGCGACAGCAGCGG
CCAAAGAATTAGTGCGGCTTGGCGTGGAAATTGCGATTATTACTTTAGCCCGTGAAGGCCTCGTCTATGCCACCGCCGAT
GGTCATGGCCATATTCCAACCTTGGTGCGCGATGTTGTCGATGCCACTGGAGCAGGCGATGCCTTGGCCGCAGCCGTCTT
ATTTGGCCTAATGAATGAGTTGACCTTGGATGAAGCTGTGCGCTTGGGAGCAAGTGCCGCCGCATTAACCGTGGCCTCAC
CCGACACCGTGCGCCGCGATTTGAGCCTAGAGAGTTTGTACGCCCAGTTGTTAATCTGA

Upstream 100 bases:

>100_bases
TTGCTGACTCTGGCGCTCGATGAGCACGAAAAACGTAAAGCCTACCGCGAACACAATCTGATCGAACAAGAATAACCATA
TAGCGAGGGAGTTATTTGGC

Downstream 100 bases:

>100_bases
GCCTAGTATTTTTGCTAGGTGCTGCCTTGGGAACAGTATGCCTGAACGTATTCTCGTTGCTGACGATAACGAAGCCCTAA
GCCAATTGCTTGAACTGATT

Product: ribokinase-like domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MEHLDTDWRVVVIGGAGLDIKGRLLEPLIEKTSNPGRLTISVGGVARNIAENLARLGAQSSLIAAVGNDEFGRLIIQQTE
DAGVDTDAMMILEDQHSAAYLALLDRNGLLYTALDDSHCARALTPDYIYDNVQLLRAADAVFIDANLARATADVILQICA
EEDIPVCFEPVAYGLAGRYRDRLRGMYLITPNEREAEALTGLPVTSPAEATAAAKELVRLGVEIAIITLAREGLVYATAD
GHGHIPTLVRDVVDATGAGDALAAAVLFGLMNELTLDEAVRLGASAAALTVASPDTVRRDLSLESLYAQLLI

Sequences:

>Translated_312_residues
MEHLDTDWRVVVIGGAGLDIKGRLLEPLIEKTSNPGRLTISVGGVARNIAENLARLGAQSSLIAAVGNDEFGRLIIQQTE
DAGVDTDAMMILEDQHSAAYLALLDRNGLLYTALDDSHCARALTPDYIYDNVQLLRAADAVFIDANLARATADVILQICA
EEDIPVCFEPVAYGLAGRYRDRLRGMYLITPNEREAEALTGLPVTSPAEATAAAKELVRLGVEIAIITLAREGLVYATAD
GHGHIPTLVRDVVDATGAGDALAAAVLFGLMNELTLDEAVRLGASAAALTVASPDTVRRDLSLESLYAQLLI
>Mature_312_residues
MEHLDTDWRVVVIGGAGLDIKGRLLEPLIEKTSNPGRLTISVGGVARNIAENLARLGAQSSLIAAVGNDEFGRLIIQQTE
DAGVDTDAMMILEDQHSAAYLALLDRNGLLYTALDDSHCARALTPDYIYDNVQLLRAADAVFIDANLARATADVILQICA
EEDIPVCFEPVAYGLAGRYRDRLRGMYLITPNEREAEALTGLPVTSPAEATAAAKELVRLGVEIAIITLAREGLVYATAD
GHGHIPTLVRDVVDATGAGDALAAAVLFGLMNELTLDEAVRLGASAAALTVASPDTVRRDLSLESLYAQLLI

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=308, Percent_Identity=26.6233766233766, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788491, Length=298, Percent_Identity=30.2013422818792, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1788484, Length=284, Percent_Identity=30.2816901408451, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790193, Length=254, Percent_Identity=26.3779527559055, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789432, Length=254, Percent_Identity=26.3779527559055, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25147470, Length=306, Percent_Identity=26.797385620915, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17532905, Length=305, Percent_Identity=25.2459016393443, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24580669, Length=311, Percent_Identity=27.3311897106109, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 33185; Mature: 33185

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLDTDWRVVVIGGAGLDIKGRLLEPLIEKTSNPGRLTISVGGVARNIAENLARLGAQS
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCC
SLIAAVGNDEFGRLIIQQTEDAGVDTDAMMILEDQHSAAYLALLDRNGLLYTALDDSHCA
CEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCHHH
RALTPDYIYDNVQLLRAADAVFIDANLARATADVILQICAEEDIPVCFEPVAYGLAGRYR
HHCCCHHHHCCHHHHHHCCEEEEECHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
DRLRGMYLITPNEREAEALTGLPVTSPAEATAAAKELVRLGVEIAIITLAREGLVYATAD
HHHCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECC
GHGHIPTLVRDVVDATGAGDALAAAVLFGLMNELTLDEAVRLGASAAALTVASPDTVRRD
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
LSLESLYAQLLI
CCHHHHHHHHCC
>Mature Secondary Structure
MEHLDTDWRVVVIGGAGLDIKGRLLEPLIEKTSNPGRLTISVGGVARNIAENLARLGAQS
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCC
SLIAAVGNDEFGRLIIQQTEDAGVDTDAMMILEDQHSAAYLALLDRNGLLYTALDDSHCA
CEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCHHH
RALTPDYIYDNVQLLRAADAVFIDANLARATADVILQICAEEDIPVCFEPVAYGLAGRYR
HHCCCHHHHCCHHHHHHCCEEEEECHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
DRLRGMYLITPNEREAEALTGLPVTSPAEATAAAKELVRLGVEIAIITLAREGLVYATAD
HHHCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECC
GHGHIPTLVRDVVDATGAGDALAAAVLFGLMNELTLDEAVRLGASAAALTVASPDTVRRD
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
LSLESLYAQLLI
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]