Click here to switch to the map view.

The map label for this gene is thiP [H]

Identifier: 159899962

GI number: 159899962

Start: 4331857

End: 4333551

Strand: Direct

Name: thiP [H]

Synonym: Haur_3445

Alternate gene names: 159899962

Gene position: 4331857-4333551 (Clockwise)

Preceding gene: 159899961

Following gene: 159899963

Centisome position: 68.25

GC content: 52.86

Gene sequence:

>1695_bases
ATGTCAAATACTCATCAGCGGCGCATTCAATGGCTGTATTGGATTTTGGCCTTGCCTGCGCTAGCTTTTTTTGTGCTCTT
TTTTGGTGTGCCACTCTGGGCAATTATGCAACGCGCGTTTGCCGAAAAAGGCCTTGGCGCGGCCATTCAAATGGTATTCA
GTAATCGCAGCCTGTTACAAGTTGTGGCTTGGAGTGCTTGGCAAGCAACGCTTTCAACAATGTTGACCTTGCTTTTGGGC
GTGCCAACCGCCTATATTTTGGCTCATTATCGATTTCCGGGGCGAGCATTGCTGCGCTCACTGATTGCCGTGCCGTTTGT
GCTGCCAACCGTTGTGGTTGCTTTGGCCTTTCGAGCACTATTGGGCGAGCGTGGCTTAATCAATCGCTGGCTCGTGGCTT
GGTTGGAGTTGGCGCAGCCGCCGTTGGAGCTTGAGCAAAGCCTTGGCATGATTTTAATCGCCCATGTTTTTTTCAATTTG
ACGGTGGTGGTGCGTTTGCTAACAGCCTATTGGAGCAATCACGATCCACGTTTAGAGGCTTCGGCTCAGGTTTTAGGTGC
GCCACGCTGGCGAGTTTGGCTCGAAGTGACCTTACCATTGGCAATGCCCGCCTTGTTGGCAGCAGCATTATTGGTGTTTA
CCTTCACGTTTTCAGCCTTCGGCACAGTGCTGTTATTGGGTAGCAGCCAGCAACGCACAATCGAAGTCGAAATTTATGAT
CAAGCGATTCATCAATTTAACTTGCCGATCGCAGCAACCCTCTCGCTCTTGCAAATTCTCACCAGTTTGGGCTTGACCTT
GGCTTATACGCGGCTGGTTCGGCGTAGCAGCGTGCCTCAAGAAGCCCAAGCATTGTCCTTACGTCGCGCTCGCACGTGGC
CAAGTCGCGTGGCAATTGGCGGGGTTATGCTACTTGCTAGCAGTTTAATCGTGCTGCCGCTGGCAAGTTTGGTGCTAGGA
GCCTTGCGGATCGAAGGTCAGTGGAGCCTTGAGTATTTTCGCATGCTGGGGATCAATCAGCGTGGCAGTTACGCCTATGT
GCCGCCAACCCAAGCCATGCTCAACTCATTGCGCTATGCGGGCATTACCACGATCTTGGCTTTAGTTTTTGGTTTGCCCT
GCGCCTATCTATTGGCCCAACCCCAAGGCCGATTAACCCGCCTGCTCGATGGCGTGTTGATGCTGCCCTTAGGCACCTCG
GCGGTAACAGTCGGCTTGGGCTATATTATCGCCTTTCGCTCATATGAATTTTGGGGCTGGGAAACGCCTGATTTGCGGCG
TTGGTCGGGCTTGTTGCCGCTAGCTCACACCCTATTAGCCTTGCCGTTTGTGATTCGCACCATGGTGCCAGCCTTGCGCC
GTTTGAACCCACAATTGCGCGAAGCTGCGCGGATGTTGGGTGCAAAACCATGGCAGGCTTGGCGTGAAGTTGATTTAGGC
TTGCTGTTGCCAAGCATCATGGCCGCAGGCTTATTTGCCTTCACTGTATCGTTGGGCGATTTTGGAGCAGCACTGGTGGT
AAGCGTAACCAGCCCAGCCACCGCCACCATGCCAGTCGTGATCTTTCGCTTTTTAGGCCAACCAGGAGCCAGCAACTATG
GCCAAGCCTTAGCGATGAGCAGCCTTTTGATGGGTGTGACCTTCATAAGTTTTCTGCTTTTAGAGCGATTTCGCGACCAA
ACCAGCGAATGGTGA

Upstream 100 bases:

>100_bases
CCTTATTGGAAACATGGTTTAGCCCCGAACTACGCGCCCAAGTCGCCGAACGGATCGAATGGCGCTAACATCGCTCCAGC
TTGGCTAGTTGAGAAACTTT

Downstream 100 bases:

>100_bases
TCGCTAGACAGCATAGAGTATAATAGCAATGGAATCAGTTGGCTTGGCTAAGGAAGTTTGCATGAAACGCGGGTCGGTAC
GCCGTACAATGATCACAATT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MSNTHQRRIQWLYWILALPALAFFVLFFGVPLWAIMQRAFAEKGLGAAIQMVFSNRSLLQVVAWSAWQATLSTMLTLLLG
VPTAYILAHYRFPGRALLRSLIAVPFVLPTVVVALAFRALLGERGLINRWLVAWLELAQPPLELEQSLGMILIAHVFFNL
TVVVRLLTAYWSNHDPRLEASAQVLGAPRWRVWLEVTLPLAMPALLAAALLVFTFTFSAFGTVLLLGSSQQRTIEVEIYD
QAIHQFNLPIAATLSLLQILTSLGLTLAYTRLVRRSSVPQEAQALSLRRARTWPSRVAIGGVMLLASSLIVLPLASLVLG
ALRIEGQWSLEYFRMLGINQRGSYAYVPPTQAMLNSLRYAGITTILALVFGLPCAYLLAQPQGRLTRLLDGVLMLPLGTS
AVTVGLGYIIAFRSYEFWGWETPDLRRWSGLLPLAHTLLALPFVIRTMVPALRRLNPQLREAARMLGAKPWQAWREVDLG
LLLPSIMAAGLFAFTVSLGDFGAALVVSVTSPATATMPVVIFRFLGQPGASNYGQALAMSSLLMGVTFISFLLLERFRDQ
TSEW

Sequences:

>Translated_564_residues
MSNTHQRRIQWLYWILALPALAFFVLFFGVPLWAIMQRAFAEKGLGAAIQMVFSNRSLLQVVAWSAWQATLSTMLTLLLG
VPTAYILAHYRFPGRALLRSLIAVPFVLPTVVVALAFRALLGERGLINRWLVAWLELAQPPLELEQSLGMILIAHVFFNL
TVVVRLLTAYWSNHDPRLEASAQVLGAPRWRVWLEVTLPLAMPALLAAALLVFTFTFSAFGTVLLLGSSQQRTIEVEIYD
QAIHQFNLPIAATLSLLQILTSLGLTLAYTRLVRRSSVPQEAQALSLRRARTWPSRVAIGGVMLLASSLIVLPLASLVLG
ALRIEGQWSLEYFRMLGINQRGSYAYVPPTQAMLNSLRYAGITTILALVFGLPCAYLLAQPQGRLTRLLDGVLMLPLGTS
AVTVGLGYIIAFRSYEFWGWETPDLRRWSGLLPLAHTLLALPFVIRTMVPALRRLNPQLREAARMLGAKPWQAWREVDLG
LLLPSIMAAGLFAFTVSLGDFGAALVVSVTSPATATMPVVIFRFLGQPGASNYGQALAMSSLLMGVTFISFLLLERFRDQ
TSEW
>Mature_563_residues
SNTHQRRIQWLYWILALPALAFFVLFFGVPLWAIMQRAFAEKGLGAAIQMVFSNRSLLQVVAWSAWQATLSTMLTLLLGV
PTAYILAHYRFPGRALLRSLIAVPFVLPTVVVALAFRALLGERGLINRWLVAWLELAQPPLELEQSLGMILIAHVFFNLT
VVVRLLTAYWSNHDPRLEASAQVLGAPRWRVWLEVTLPLAMPALLAAALLVFTFTFSAFGTVLLLGSSQQRTIEVEIYDQ
AIHQFNLPIAATLSLLQILTSLGLTLAYTRLVRRSSVPQEAQALSLRRARTWPSRVAIGGVMLLASSLIVLPLASLVLGA
LRIEGQWSLEYFRMLGINQRGSYAYVPPTQAMLNSLRYAGITTILALVFGLPCAYLLAQPQGRLTRLLDGVLMLPLGTSA
VTVGLGYIIAFRSYEFWGWETPDLRRWSGLLPLAHTLLALPFVIRTMVPALRRLNPQLREAARMLGAKPWQAWREVDLGL
LLPSIMAAGLFAFTVSLGDFGAALVVSVTSPATATMPVVIFRFLGQPGASNYGQALAMSSLLMGVTFISFLLLERFRDQT
SEW

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=488, Percent_Identity=26.0245901639344, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788764, Length=204, Percent_Identity=31.8627450980392, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87082099, Length=284, Percent_Identity=26.4084507042254, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787369, Length=240, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 62439; Mature: 62308

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTHQRRIQWLYWILALPALAFFVLFFGVPLWAIMQRAFAEKGLGAAIQMVFSNRSLLQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
VVAWSAWQATLSTMLTLLLGVPTAYILAHYRFPGRALLRSLIAVPFVLPTVVVALAFRAL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGERGLINRWLVAWLELAQPPLELEQSLGMILIAHVFFNLTVVVRLLTAYWSNHDPRLEA
HCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SAQVLGAPRWRVWLEVTLPLAMPALLAAALLVFTFTFSAFGTVLLLGSSQQRTIEVEIYD
HHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEHH
QAIHQFNLPIAATLSLLQILTSLGLTLAYTRLVRRSSVPQEAQALSLRRARTWPSRVAIG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
GVMLLASSLIVLPLASLVLGALRIEGQWSLEYFRMLGINQRGSYAYVPPTQAMLNSLRYA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHH
GITTILALVFGLPCAYLLAQPQGRLTRLLDGVLMLPLGTSAVTVGLGYIIAFRSYEFWGW
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
ETPDLRRWSGLLPLAHTLLALPFVIRTMVPALRRLNPQLREAARMLGAKPWQAWREVDLG
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
LLLPSIMAAGLFAFTVSLGDFGAALVVSVTSPATATMPVVIFRFLGQPGASNYGQALAMS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
SLLMGVTFISFLLLERFRDQTSEW
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNTHQRRIQWLYWILALPALAFFVLFFGVPLWAIMQRAFAEKGLGAAIQMVFSNRSLLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
VVAWSAWQATLSTMLTLLLGVPTAYILAHYRFPGRALLRSLIAVPFVLPTVVVALAFRAL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGERGLINRWLVAWLELAQPPLELEQSLGMILIAHVFFNLTVVVRLLTAYWSNHDPRLEA
HCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SAQVLGAPRWRVWLEVTLPLAMPALLAAALLVFTFTFSAFGTVLLLGSSQQRTIEVEIYD
HHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEHH
QAIHQFNLPIAATLSLLQILTSLGLTLAYTRLVRRSSVPQEAQALSLRRARTWPSRVAIG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
GVMLLASSLIVLPLASLVLGALRIEGQWSLEYFRMLGINQRGSYAYVPPTQAMLNSLRYA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHH
GITTILALVFGLPCAYLLAQPQGRLTRLLDGVLMLPLGTSAVTVGLGYIIAFRSYEFWGW
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
ETPDLRRWSGLLPLAHTLLALPFVIRTMVPALRRLNPQLREAARMLGAKPWQAWREVDLG
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
LLLPSIMAAGLFAFTVSLGDFGAALVVSVTSPATATMPVVIFRFLGQPGASNYGQALAMS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
SLLMGVTFISFLLLERFRDQTSEW
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]