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The map label for this gene is gacS [H]

Identifier: 159899963

GI number: 159899963

Start: 4333613

End: 4335988

Strand: Direct

Name: gacS [H]

Synonym: Haur_3446

Alternate gene names: 159899963

Gene position: 4333613-4335988 (Clockwise)

Preceding gene: 159899962

Following gene: 159899964

Centisome position: 68.28

GC content: 50.59

Gene sequence:

>2376_bases
ATGAAACGCGGGTCGGTACGCCGTACAATGATCACAATTGCAGCGATCATTTTGAGCTTAATGACCGGATTAAATATCAT
CGGGGTTGTTGAGCGTAGCATGCTCAATAATGATATTGAATATGTGATCGATTTAGAAGATGCCGAACGTTTAGCTCGCG
AAATTAGCCTCTACACTCAATATCAAGCCCATGCGCTCGATGCCTATGCCTTAGGTGAAATCGAGGAGCGTGAACACTAT
ACCCGTTATCGCCAAGCCTTCGATGACAAACGCCTAGAGCTTGAACAATTCTTCAAGAACATGCAGCCGAACCCAGAAAC
CAAAACAGCCTTTGAGAATGTACAAAAACTTAGCGCCGACTATGAAGATGCCGGAGTTGCCTACCTCGCCCAAATCGACC
TACGCATCCAAGAATCAGCGCCAACCCGCTCAACCGCAGAACTGGCTGCTTGGAAAATCCTCGACGAACAGGCCGATCAA
CTCGACGAGGCCACCCAAGTGCTCTCTGATATCATTGATGATCAATCTGAAGCACTTGAAGCAACAATTACCAAGCAAAA
TGGCCGCATGATTGTGGCACTGACTGGGCGCAGTTTGGCGATACTAGTATTGCTAAGTCTGTTCGTCTACTATCTGCTGG
GGAGGGTCGGCAATCAATTCAAGTTGGTACGCGACGGAGCGCAACGCTTTGCCGATGGCGATTTTACCACTGATATTCCA
ATTCGCCGCTATGATGAAGTAGGCCGCCTCGCTGCGATGTTCAATACCATGGCCCAAACGATTCGTGGCCAAATCGAGCG
ACTTGAGCAAGCCAAAGATCATGCCCAACGCTTGCAATTTGTGGCCGAAGAAGCCAATCGCGCCAAAAGTAATTTCTTGG
CCAATATGAGCCACGAATTGCGCACCCCACTCAATGCGATCATCGGCTATAGCGAAATTCTCCAAGAAGAATGTGAAGAC
CTCGGCCAAACCGCAATGATCGAAGATCTCGATCGGATTCGCCTCTCAGGGCGGCATCTGCTGACCTTGATCAACGATAT
TTTGGATTTGGCCAAGATTGAATCGGGCAAGGTTGAAATTTTGCCTGAGGAAATTTCGCTGCCCCAACTGCTGCACGATG
TGCGCTCAACCGTCGATCCGATGATCATCAAAAATGAAAATCGCTTGGTGATCGAATCAGCAGCTGGCTTGCTGACGATG
ATTAGCGACGAGACCCGTTTACGCCAGATTTTGGTCAATTTGCTGAGCAACGCAGCGAAATTCACCGAACATGGCCGCAT
TACCTTGCGCGTCCAACCAAGCGAAGAAGAGGGCTGGATTGATTTCAGCGTGCATGATAATGGCATTGGCATGAGCAACG
CCCAATTATCACGCTTGTTTCAGCCATTTACCCAAGCCGATGCCTCGACCACCCGCAAATATGGCGGCACTGGGCTGGGT
TTGGCCTTAAGTCGGCGCTTGGCTCAATTGCTCGGCGGCGATATTCGGGTACAAAGTGAATTAGGCGTTGGCTCAACCTT
CAGCGTGCACCTACCGCAATCAGTCATCGATATGGCTCCAGTTTCGTTACTTGATGAAGCGCCAGTTATTATCAGCGATG
CCAACAACAACCAACCCAAAGTGCTGATTATCGACGATGATCGCAATGTCCATCATCTGCTTTCGCGCACGCTCAAGCGC
GAGGGCTGGAGCGTCCTTAGCGCATTTGATGGCGAAAATGGCTTAGCAATGGTGCGCAATCATCATCCAACGGCAATTTT
GCTCGATGTGTTGTTGCCAGGCCATGTCAATGGTTGGGAGATTTTGGCCGAAATCAAGGCCGACCCCAAAATTGCCACAA
TTCCGGTAATTATGCATACGATTGTGGCCGAGCCAAACCAAGGGGTTTCGTTTGGGGTGTACGATTATTTGATTAAGCCC
GTTGATCGCGGCCAATTGCTGCGCACGCTACGGAGTTGTATCGACCCGCAAAATGCCAAAACCCAATTGGTTTTGGTGGT
CGATGACGATCATGATAGTCGGGCGATGCTGCGACGCATGCTCGAAGGCGCTGGCTGGAAAGTCTATGAGGCTGCCAACG
GGCGCGAAGCCTTGGGCGCATTGCATAGCCGCCCATTTGGCGCGATGATTCTCGATCTAATGATGCCCGAAATGGATGGC
TTCGAAACGATCGCTGCCTTACAAGAGCTTGAGCAATTCCGCGATTTGCCGATTATTGTGGTTTCGGCCAAAGAACTCAC
TGAAGAAGAGCGGCAACAGCTTGAAGAGACCGTTGAACGGGTGGTCAGTAAGGGGAATGTGCGGCGTGAAGAGATTTTGG
CGTTGGTGCGCGAGCAAGTTCGGCGGCGCGTTGAGCAACCGCCTACAACCACGTAA

Upstream 100 bases:

>100_bases
CTTTTAGAGCGATTTCGCGACCAAACCAGCGAATGGTGATCGCTAGACAGCATAGAGTATAATAGCAATGGAATCAGTTG
GCTTGGCTAAGGAAGTTTGC

Downstream 100 bases:

>100_bases
ATTGCTTAAGCTGGGCTTACGCTTGGCATTGCTGGCAATTTTAGGTCTGTTGCTGGTCAATGGTTGGGTGCGCTGGGCAA
CCGCCGATTATCGTTTTGCT

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MKRGSVRRTMITIAAIILSLMTGLNIIGVVERSMLNNDIEYVIDLEDAERLAREISLYTQYQAHALDAYALGEIEEREHY
TRYRQAFDDKRLELEQFFKNMQPNPETKTAFENVQKLSADYEDAGVAYLAQIDLRIQESAPTRSTAELAAWKILDEQADQ
LDEATQVLSDIIDDQSEALEATITKQNGRMIVALTGRSLAILVLLSLFVYYLLGRVGNQFKLVRDGAQRFADGDFTTDIP
IRRYDEVGRLAAMFNTMAQTIRGQIERLEQAKDHAQRLQFVAEEANRAKSNFLANMSHELRTPLNAIIGYSEILQEECED
LGQTAMIEDLDRIRLSGRHLLTLINDILDLAKIESGKVEILPEEISLPQLLHDVRSTVDPMIIKNENRLVIESAAGLLTM
ISDETRLRQILVNLLSNAAKFTEHGRITLRVQPSEEEGWIDFSVHDNGIGMSNAQLSRLFQPFTQADASTTRKYGGTGLG
LALSRRLAQLLGGDIRVQSELGVGSTFSVHLPQSVIDMAPVSLLDEAPVIISDANNNQPKVLIIDDDRNVHHLLSRTLKR
EGWSVLSAFDGENGLAMVRNHHPTAILLDVLLPGHVNGWEILAEIKADPKIATIPVIMHTIVAEPNQGVSFGVYDYLIKP
VDRGQLLRTLRSCIDPQNAKTQLVLVVDDDHDSRAMLRRMLEGAGWKVYEAANGREALGALHSRPFGAMILDLMMPEMDG
FETIAALQELEQFRDLPIIVVSAKELTEEERQQLEETVERVVSKGNVRREEILALVREQVRRRVEQPPTTT

Sequences:

>Translated_791_residues
MKRGSVRRTMITIAAIILSLMTGLNIIGVVERSMLNNDIEYVIDLEDAERLAREISLYTQYQAHALDAYALGEIEEREHY
TRYRQAFDDKRLELEQFFKNMQPNPETKTAFENVQKLSADYEDAGVAYLAQIDLRIQESAPTRSTAELAAWKILDEQADQ
LDEATQVLSDIIDDQSEALEATITKQNGRMIVALTGRSLAILVLLSLFVYYLLGRVGNQFKLVRDGAQRFADGDFTTDIP
IRRYDEVGRLAAMFNTMAQTIRGQIERLEQAKDHAQRLQFVAEEANRAKSNFLANMSHELRTPLNAIIGYSEILQEECED
LGQTAMIEDLDRIRLSGRHLLTLINDILDLAKIESGKVEILPEEISLPQLLHDVRSTVDPMIIKNENRLVIESAAGLLTM
ISDETRLRQILVNLLSNAAKFTEHGRITLRVQPSEEEGWIDFSVHDNGIGMSNAQLSRLFQPFTQADASTTRKYGGTGLG
LALSRRLAQLLGGDIRVQSELGVGSTFSVHLPQSVIDMAPVSLLDEAPVIISDANNNQPKVLIIDDDRNVHHLLSRTLKR
EGWSVLSAFDGENGLAMVRNHHPTAILLDVLLPGHVNGWEILAEIKADPKIATIPVIMHTIVAEPNQGVSFGVYDYLIKP
VDRGQLLRTLRSCIDPQNAKTQLVLVVDDDHDSRAMLRRMLEGAGWKVYEAANGREALGALHSRPFGAMILDLMMPEMDG
FETIAALQELEQFRDLPIIVVSAKELTEEERQQLEETVERVVSKGNVRREEILALVREQVRRRVEQPPTTT
>Mature_791_residues
MKRGSVRRTMITIAAIILSLMTGLNIIGVVERSMLNNDIEYVIDLEDAERLAREISLYTQYQAHALDAYALGEIEEREHY
TRYRQAFDDKRLELEQFFKNMQPNPETKTAFENVQKLSADYEDAGVAYLAQIDLRIQESAPTRSTAELAAWKILDEQADQ
LDEATQVLSDIIDDQSEALEATITKQNGRMIVALTGRSLAILVLLSLFVYYLLGRVGNQFKLVRDGAQRFADGDFTTDIP
IRRYDEVGRLAAMFNTMAQTIRGQIERLEQAKDHAQRLQFVAEEANRAKSNFLANMSHELRTPLNAIIGYSEILQEECED
LGQTAMIEDLDRIRLSGRHLLTLINDILDLAKIESGKVEILPEEISLPQLLHDVRSTVDPMIIKNENRLVIESAAGLLTM
ISDETRLRQILVNLLSNAAKFTEHGRITLRVQPSEEEGWIDFSVHDNGIGMSNAQLSRLFQPFTQADASTTRKYGGTGLG
LALSRRLAQLLGGDIRVQSELGVGSTFSVHLPQSVIDMAPVSLLDEAPVIISDANNNQPKVLIIDDDRNVHHLLSRTLKR
EGWSVLSAFDGENGLAMVRNHHPTAILLDVLLPGHVNGWEILAEIKADPKIATIPVIMHTIVAEPNQGVSFGVYDYLIKP
VDRGQLLRTLRSCIDPQNAKTQLVLVVDDDHDSRAMLRRMLEGAGWKVYEAANGREALGALHSRPFGAMILDLMMPEMDG
FETIAALQELEQFRDLPIIVVSAKELTEEERQQLEETVERVVSKGNVRREEILALVREQVRRRVEQPPTTT

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=520, Percent_Identity=29.4230769230769, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081816, Length=444, Percent_Identity=33.3333333333333, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI48994928, Length=428, Percent_Identity=34.1121495327103, Blast_Score=169, Evalue=7e-43,
Organism=Escherichia coli, GI1789149, Length=264, Percent_Identity=40.9090909090909, Blast_Score=161, Evalue=2e-40,
Organism=Escherichia coli, GI1788713, Length=368, Percent_Identity=30.1630434782609, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=32.1739130434783, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI1788393, Length=292, Percent_Identity=26.7123287671233, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1786912, Length=262, Percent_Identity=27.4809160305344, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=30.3964757709251, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1790436, Length=240, Percent_Identity=29.5833333333333, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=262, Percent_Identity=29.0076335877863, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790551, Length=209, Percent_Identity=29.6650717703349, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1787894, Length=292, Percent_Identity=26.3698630136986, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1790346, Length=226, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=222, Percent_Identity=27.027027027027, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI87082128, Length=246, Percent_Identity=27.6422764227642, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=42.6666666666667, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 88800; Mature: 88800

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50885 HAMP ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRGSVRRTMITIAAIILSLMTGLNIIGVVERSMLNNDIEYVIDLEDAERLAREISLYTQ
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
YQAHALDAYALGEIEEREHYTRYRQAFDDKRLELEQFFKNMQPNPETKTAFENVQKLSAD
HHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
YEDAGVAYLAQIDLRIQESAPTRSTAELAAWKILDEQADQLDEATQVLSDIIDDQSEALE
CCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ATITKQNGRMIVALTGRSLAILVLLSLFVYYLLGRVGNQFKLVRDGAQRFADGDFTTDIP
HEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
IRRYDEVGRLAAMFNTMAQTIRGQIERLEQAKDHAQRLQFVAEEANRAKSNFLANMSHEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTPLNAIIGYSEILQEECEDLGQTAMIEDLDRIRLSGRHLLTLINDILDLAKIESGKVEI
HCHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEE
LPEEISLPQLLHDVRSTVDPMIIKNENRLVIESAAGLLTMISDETRLRQILVNLLSNAAK
CCCCCCHHHHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHH
FTEHGRITLRVQPSEEEGWIDFSVHDNGIGMSNAQLSRLFQPFTQADASTTRKYGGTGLG
HHHCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHH
LALSRRLAQLLGGDIRVQSELGVGSTFSVHLPQSVIDMAPVSLLDEAPVIISDANNNQPK
HHHHHHHHHHHCCCEEEEECCCCCCEEEEECCHHHHHHCCHHHHCCCCEEEECCCCCCCE
VLIIDDDRNVHHLLSRTLKREGWSVLSAFDGENGLAMVRNHHPTAILLDVLLPGHVNGWE
EEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCEEEEEHHCCCCCCHHH
ILAEIKADPKIATIPVIMHTIVAEPNQGVSFGVYDYLIKPVDRGQLLRTLRSCIDPQNAK
HHEECCCCCCEEHHHHHHHHHHCCCCCCCEECHHHHHHCCCCHHHHHHHHHHHCCCCCCC
TQLVLVVDDDHDSRAMLRRMLEGAGWKVYEAANGREALGALHSRPFGAMILDLMMPEMDG
EEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCCC
FETIAALQELEQFRDLPIIVVSAKELTEEERQQLEETVERVVSKGNVRREEILALVREQV
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RRRVEQPPTTT
HHHHCCCCCCC
>Mature Secondary Structure
MKRGSVRRTMITIAAIILSLMTGLNIIGVVERSMLNNDIEYVIDLEDAERLAREISLYTQ
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
YQAHALDAYALGEIEEREHYTRYRQAFDDKRLELEQFFKNMQPNPETKTAFENVQKLSAD
HHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
YEDAGVAYLAQIDLRIQESAPTRSTAELAAWKILDEQADQLDEATQVLSDIIDDQSEALE
CCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ATITKQNGRMIVALTGRSLAILVLLSLFVYYLLGRVGNQFKLVRDGAQRFADGDFTTDIP
HEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
IRRYDEVGRLAAMFNTMAQTIRGQIERLEQAKDHAQRLQFVAEEANRAKSNFLANMSHEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTPLNAIIGYSEILQEECEDLGQTAMIEDLDRIRLSGRHLLTLINDILDLAKIESGKVEI
HCHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEE
LPEEISLPQLLHDVRSTVDPMIIKNENRLVIESAAGLLTMISDETRLRQILVNLLSNAAK
CCCCCCHHHHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHH
FTEHGRITLRVQPSEEEGWIDFSVHDNGIGMSNAQLSRLFQPFTQADASTTRKYGGTGLG
HHHCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHH
LALSRRLAQLLGGDIRVQSELGVGSTFSVHLPQSVIDMAPVSLLDEAPVIISDANNNQPK
HHHHHHHHHHHCCCEEEEECCCCCCEEEEECCHHHHHHCCHHHHCCCCEEEECCCCCCCE
VLIIDDDRNVHHLLSRTLKREGWSVLSAFDGENGLAMVRNHHPTAILLDVLLPGHVNGWE
EEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCEEEEEHHCCCCCCHHH
ILAEIKADPKIATIPVIMHTIVAEPNQGVSFGVYDYLIKPVDRGQLLRTLRSCIDPQNAK
HHEECCCCCCEEHHHHHHHHHHCCCCCCCEECHHHHHHCCCCHHHHHHHHHHHCCCCCCC
TQLVLVVDDDHDSRAMLRRMLEGAGWKVYEAANGREALGALHSRPFGAMILDLMMPEMDG
EEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCCC
FETIAALQELEQFRDLPIIVVSAKELTEEERQQLEETVERVVSKGNVRREEILALVREQV
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RRRVEQPPTTT
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]