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The map label for this gene is walK [H]

Identifier: 159899857

GI number: 159899857

Start: 4210619

End: 4212292

Strand: Direct

Name: walK [H]

Synonym: Haur_3340

Alternate gene names: 159899857

Gene position: 4210619-4212292 (Clockwise)

Preceding gene: 159899856

Following gene: 159899858

Centisome position: 66.34

GC content: 55.08

Gene sequence:

>1674_bases
ATGACTGAAAGCGTCGCAATGACTGAGCAGCCTAGTCAAACAGCTGCGATAACTCTCCCACGTTCGCCCTTAAGTCGGTT
GTTGTTGCCAATTGCCTTGGTGGTGTTGGCGCTTGGTGCTGGGCTGGCAGCGTGGAGCGGATTTAATTTTGGCCCACAAA
CGATGCTGATCGGGGCGATTGCAGGCGGAGTTGGCGCACTGGCCGCTTTGGTTGGCCTGATTGCTGGCTTGCGTCGGCGC
TTGCCCTTGAGTGGTTGGGCCTTGATTGTGGGTACGCTCAGCATCGCTTTGGGTGCAACCATGGTCTTCCCTGAACAACG
CGAATTGGCGATTATCAGCGCCGCCCTGCCGGTTTTGTGTGCCGCGCTGACCTTGCGCCCCTTGCACACTGCTATTGTTA
CGGTTGTTAGTTTAGCGAGTTTATGGGCGGCGGCCTATTTTGCTGCCGCCGAGCCAAGCACTAATCAGTTAATTCAATGG
AGCTTGCTCAGTGCCTTTGTGCTCGTGATCAGCATTTTTGCCTTGGTTTGTAGCGCAGCGCTCCGTGCTTTTGATCGCCA
GCAAACTGTCGGACATGAATCAACCACCCAACTCAATAAGCAACTTGGCGAAGAGCAAGAACGGGTCGAACGCGCCGCCC
AATTGTTGGTGCAAGAGCGTGATCGACTGGCGGCGGTGCTGGGCGCAGCGAGCGATGGCGTGGTGTTGGCCGATAGCAAT
GGCATCATTTTGCAGGCCAATGCTGCTGCCCGCCAACTGTTGAATGAAGCGTTTGGCGGCACGCTCGAAGGCCAAGCACT
CAACCAGTGGAGCCAAGAAAACACGGGCCGTTTGCGGGTAATTAGCAACGAACGCGAAGGCGAACGCCAACGGGTGGTTT
TTGAGCAACAACAAGGCACGCGCAAACCCGTAATTGGCCTTAACCAAGTGCCAATTCGCAGTAGCGCCGGCAGTGTCTTG
GGCTATGTTGGGGTTTTCCACGATAAAACCACCGAATTAGAAGTTGAAGAAATGCGTTCGCAATTACTCGATTTCCTCGT
GCAAGATATGCATGATCCGCTCAACTCGGTGTTGGCAGCCCAAGATACCTTGTTGGCTGGCGATTTAGGCGATGGCAATG
AGCGGGTTTTATCGACCGCCCGCCGCACAACCTCGCGCTTGGTTGAGCTAACCAATACCTTGATGGAAATGAGCCGTTTG
CATGGCGACCCCAATAGCCTGCATCGTTTGGCCAACCCATTGCGCCCATTGATCGAAGGCAGCATCGCCCAATCAACACC
ACAAGCCCAACAACGGGCAATCAACTTGGTGTTGGAGTATGGCGCAGATAGTGGTGGCCTCGCCTTCGATGCTGATAAGA
TGCGGCGGGTGATGAGCCACTTGCTTGATAATGCTTTGCGTCGCAGCCCAGCCTATAGCACGGTGCGGGTGCAAGTGAGC
AACACGGGCGGAAATGCCCAAGTACGGATCGCCGACCAAGGCCCAAGTATTCCGGTGGAACTCGCAGGCCGCATTTTTGA
TCGCTTCAGCAAACAGGTTGGCGAACAACGGATCGGCGGGGTTGGCCTAGCCTATTGCAAACAGGTGATTGAGGCTCATG
GTGGCCGAATTTGGGTCGATAGCACGCCTGGCAAAGGCAGTACCTTTATCTTTAGCATGCCCTCAGCAGCATAA

Upstream 100 bases:

>100_bases
GCCACAACTCAAGGCACCACCGAAGCAGCCAATTACAGCTATAACGTCACACCGTAGTATAATTGGGCAGCCCTAGCTTA
GATGGATTGAGGATTGGCTC

Downstream 100 bases:

>100_bases
TCTAGGAGTAGTTCCATGGCAAAAGTAGGCCGCAACGACCCCTGCCCATGTGGCAGTGGCAAAAAATATAAGCAATGTCA
TGAAGCAGCTGACCGTGCGC

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTESVAMTEQPSQTAAITLPRSPLSRLLLPIALVVLALGAGLAAWSGFNFGPQTMLIGAIAGGVGALAALVGLIAGLRRR
LPLSGWALIVGTLSIALGATMVFPEQRELAIISAALPVLCAALTLRPLHTAIVTVVSLASLWAAAYFAAAEPSTNQLIQW
SLLSAFVLVISIFALVCSAALRAFDRQQTVGHESTTQLNKQLGEEQERVERAAQLLVQERDRLAAVLGAASDGVVLADSN
GIILQANAAARQLLNEAFGGTLEGQALNQWSQENTGRLRVISNEREGERQRVVFEQQQGTRKPVIGLNQVPIRSSAGSVL
GYVGVFHDKTTELEVEEMRSQLLDFLVQDMHDPLNSVLAAQDTLLAGDLGDGNERVLSTARRTTSRLVELTNTLMEMSRL
HGDPNSLHRLANPLRPLIEGSIAQSTPQAQQRAINLVLEYGADSGGLAFDADKMRRVMSHLLDNALRRSPAYSTVRVQVS
NTGGNAQVRIADQGPSIPVELAGRIFDRFSKQVGEQRIGGVGLAYCKQVIEAHGGRIWVDSTPGKGSTFIFSMPSAA

Sequences:

>Translated_557_residues
MTESVAMTEQPSQTAAITLPRSPLSRLLLPIALVVLALGAGLAAWSGFNFGPQTMLIGAIAGGVGALAALVGLIAGLRRR
LPLSGWALIVGTLSIALGATMVFPEQRELAIISAALPVLCAALTLRPLHTAIVTVVSLASLWAAAYFAAAEPSTNQLIQW
SLLSAFVLVISIFALVCSAALRAFDRQQTVGHESTTQLNKQLGEEQERVERAAQLLVQERDRLAAVLGAASDGVVLADSN
GIILQANAAARQLLNEAFGGTLEGQALNQWSQENTGRLRVISNEREGERQRVVFEQQQGTRKPVIGLNQVPIRSSAGSVL
GYVGVFHDKTTELEVEEMRSQLLDFLVQDMHDPLNSVLAAQDTLLAGDLGDGNERVLSTARRTTSRLVELTNTLMEMSRL
HGDPNSLHRLANPLRPLIEGSIAQSTPQAQQRAINLVLEYGADSGGLAFDADKMRRVMSHLLDNALRRSPAYSTVRVQVS
NTGGNAQVRIADQGPSIPVELAGRIFDRFSKQVGEQRIGGVGLAYCKQVIEAHGGRIWVDSTPGKGSTFIFSMPSAA
>Mature_556_residues
TESVAMTEQPSQTAAITLPRSPLSRLLLPIALVVLALGAGLAAWSGFNFGPQTMLIGAIAGGVGALAALVGLIAGLRRRL
PLSGWALIVGTLSIALGATMVFPEQRELAIISAALPVLCAALTLRPLHTAIVTVVSLASLWAAAYFAAAEPSTNQLIQWS
LLSAFVLVISIFALVCSAALRAFDRQQTVGHESTTQLNKQLGEEQERVERAAQLLVQERDRLAAVLGAASDGVVLADSNG
IILQANAAARQLLNEAFGGTLEGQALNQWSQENTGRLRVISNEREGERQRVVFEQQQGTRKPVIGLNQVPIRSSAGSVLG
YVGVFHDKTTELEVEEMRSQLLDFLVQDMHDPLNSVLAAQDTLLAGDLGDGNERVLSTARRTTSRLVELTNTLMEMSRLH
GDPNSLHRLANPLRPLIEGSIAQSTPQAQQRAINLVLEYGADSGGLAFDADKMRRVMSHLLDNALRRSPAYSTVRVQVSN
TGGNAQVRIADQGPSIPVELAGRIFDRFSKQVGEQRIGGVGLAYCKQVIEAHGGRIWVDSTPGKGSTFIFSMPSAA

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=29.646017699115, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788549, Length=375, Percent_Identity=24, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693157, Length=222, Percent_Identity=26.5765765765766, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1786600, Length=348, Percent_Identity=24.7126436781609, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790567, Length=345, Percent_Identity=25.7971014492754, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1786783, Length=131, Percent_Identity=33.587786259542, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=23.5807860262009, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=376, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790436, Length=173, Percent_Identity=27.7456647398844, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59516; Mature: 59385

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESVAMTEQPSQTAAITLPRSPLSRLLLPIALVVLALGAGLAAWSGFNFGPQTMLIGAI
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
AGGVGALAALVGLIAGLRRRLPLSGWALIVGTLSIALGATMVFPEQRELAIISAALPVLC
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
AALTLRPLHTAIVTVVSLASLWAAAYFAAAEPSTNQLIQWSLLSAFVLVISIFALVCSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAFDRQQTVGHESTTQLNKQLGEEQERVERAAQLLVQERDRLAAVLGAASDGVVLADSN
HHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
GIILQANAAARQLLNEAFGGTLEGQALNQWSQENTGRLRVISNEREGERQRVVFEQQQGT
CEEEEECHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCC
RKPVIGLNQVPIRSSAGSVLGYVGVFHDKTTELEVEEMRSQLLDFLVQDMHDPLNSVLAA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDTLLAGDLGDGNERVLSTARRTTSRLVELTNTLMEMSRLHGDPNSLHRLANPLRPLIEG
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
SIAQSTPQAQQRAINLVLEYGADSGGLAFDADKMRRVMSHLLDNALRRSPAYSTVRVQVS
HHHCCCHHHHHHHHHHHHEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
NTGGNAQVRIADQGPSIPVELAGRIFDRFSKQVGEQRIGGVGLAYCKQVIEAHGGRIWVD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEE
STPGKGSTFIFSMPSAA
CCCCCCCEEEEECCCCC
>Mature Secondary Structure 
TESVAMTEQPSQTAAITLPRSPLSRLLLPIALVVLALGAGLAAWSGFNFGPQTMLIGAI
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
AGGVGALAALVGLIAGLRRRLPLSGWALIVGTLSIALGATMVFPEQRELAIISAALPVLC
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
AALTLRPLHTAIVTVVSLASLWAAAYFAAAEPSTNQLIQWSLLSAFVLVISIFALVCSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAFDRQQTVGHESTTQLNKQLGEEQERVERAAQLLVQERDRLAAVLGAASDGVVLADSN
HHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
GIILQANAAARQLLNEAFGGTLEGQALNQWSQENTGRLRVISNEREGERQRVVFEQQQGT
CEEEEECHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCC
RKPVIGLNQVPIRSSAGSVLGYVGVFHDKTTELEVEEMRSQLLDFLVQDMHDPLNSVLAA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDTLLAGDLGDGNERVLSTARRTTSRLVELTNTLMEMSRLHGDPNSLHRLANPLRPLIEG
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
SIAQSTPQAQQRAINLVLEYGADSGGLAFDADKMRRVMSHLLDNALRRSPAYSTVRVQVS
HHHCCCHHHHHHHHHHHHEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
NTGGNAQVRIADQGPSIPVELAGRIFDRFSKQVGEQRIGGVGLAYCKQVIEAHGGRIWVD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEE
STPGKGSTFIFSMPSAA
CCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA