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The map label for this gene is leuD [H]

Identifier: 159899820

GI number: 159899820

Start: 4169428

End: 4169925

Strand: Direct

Name: leuD [H]

Synonym: Haur_3303

Alternate gene names: 159899820

Gene position: 4169428-4169925 (Clockwise)

Preceding gene: 159899819

Following gene: 159899821

Centisome position: 65.7

GC content: 54.42

Gene sequence:

>498_bases
ATGCCACGGATTTGGGTTTTTGGAAATGATGTCAACACCGACCAGATGGTTCCAGGCCGTTATGCTCCCTACATGCTCGG
CGCAAACGATGATGTGCGACGCTATGCCTTTATCGAAGCGCGGCCCGATTTTGCCCCCAACGTTCAGCCAGGCGATTTAA
TTATTGCTGGGCCAAACTTTGGCTGTGGCTCAAGCCGCGAGTATGCTCCCTTGGCCTTGAAGCGCTGCGGGGTTGGCGCA
ATTATCGCTCCGCTGTTTGCCCGCATTTTCTTCCGCAACGCCTTGAACTTGGGCATTCCCTGTTTCACCGCCGATCTTCG
CGAGCAAGTTGCTGATGGCGATTTTGTGGATTTTGATTTGGCTGCTGGCCTGATCACCAACAATGCCAACCAAATTCAAC
TACCACCACCAGAAGGCTGGATGCGTGAAGTCTGGGCTGAAGGCGGAATCGTGCCATTCTACCGTCGCCATGGTCGCTTT
CCCGCGACAGGAGCCTAA

Upstream 100 bases:

>100_bases
ACCGCCGTCACGGGCTACATCACCCACCCGCGCAATGTGCTCGATAGCACTGAGCAAGCAGTTTTCGCCTAATTTTTGCA
ATTGCACAGGAGTCAGCACT

Downstream 100 bases:

>100_bases
ACATGACCAGGTTGTGTTTGATTGCTGGCGATGGGATTGGCCGCGAAGTGGTGCAAGCAGCCCGCCAAGTGCTCGAAGCC
TTAGCAGTTCCTGCCGAGTT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MPRIWVFGNDVNTDQMVPGRYAPYMLGANDDVRRYAFIEARPDFAPNVQPGDLIIAGPNFGCGSSREYAPLALKRCGVGA
IIAPLFARIFFRNALNLGIPCFTADLREQVADGDFVDFDLAAGLITNNANQIQLPPPEGWMREVWAEGGIVPFYRRHGRF
PATGA

Sequences:

>Translated_165_residues
MPRIWVFGNDVNTDQMVPGRYAPYMLGANDDVRRYAFIEARPDFAPNVQPGDLIIAGPNFGCGSSREYAPLALKRCGVGA
IIAPLFARIFFRNALNLGIPCFTADLREQVADGDFVDFDLAAGLITNNANQIQLPPPEGWMREVWAEGGIVPFYRRHGRF
PATGA
>Mature_164_residues
PRIWVFGNDVNTDQMVPGRYAPYMLGANDDVRRYAFIEARPDFAPNVQPGDLIIAGPNFGCGSSREYAPLALKRCGVGAI
IAPLFARIFFRNALNLGIPCFTADLREQVADGDFVDFDLAAGLITNNANQIQLPPPEGWMREVWAEGGIVPFYRRHGRFP
ATGA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=112, Percent_Identity=34.8214285714286, Blast_Score=68, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321429, Length=105, Percent_Identity=35.2380952380952, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18175; Mature: 18044

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIWVFGNDVNTDQMVPGRYAPYMLGANDDVRRYAFIEARPDFAPNVQPGDLIIAGPNF
CCEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCC
GCGSSREYAPLALKRCGVGAIIAPLFARIFFRNALNLGIPCFTADLREQVADGDFVDFDL
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCCEEEHHH
AAGLITNNANQIQLPPPEGWMREVWAEGGIVPFYRRHGRFPATGA
HHHEEECCCCEEEECCCCHHHHHHHHCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
PRIWVFGNDVNTDQMVPGRYAPYMLGANDDVRRYAFIEARPDFAPNVQPGDLIIAGPNF
CEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCC
GCGSSREYAPLALKRCGVGAIIAPLFARIFFRNALNLGIPCFTADLREQVADGDFVDFDL
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCCEEEHHH
AAGLITNNANQIQLPPPEGWMREVWAEGGIVPFYRRHGRFPATGA
HHHEEECCCCEEEECCCCHHHHHHHHCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]