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The map label for this gene is aksF [H]

Identifier: 159899821

GI number: 159899821

Start: 4169928

End: 4170923

Strand: Direct

Name: aksF [H]

Synonym: Haur_3304

Alternate gene names: 159899821

Gene position: 4169928-4170923 (Clockwise)

Preceding gene: 159899820

Following gene: 159899822

Centisome position: 65.7

GC content: 55.72

Gene sequence:

>996_bases
ATGACCAGGTTGTGTTTGATTGCTGGCGATGGGATTGGCCGCGAAGTGGTGCAAGCAGCCCGCCAAGTGCTCGAAGCCTT
AGCAGTTCCTGCCGAGTTTGTTGAGGCCGAGGCTGGTTGGGAAACCTTTCAGCGCACTGGCAACGCCTTGCCCGAACAAA
CTTTAGCGGCTGTCCAAGCGGCCAATTCAACCTTGTTTGGCGCAGTTAGTTCGCCATCGCAGCGGGTCGCTGGCTATCGC
AGTCCAATTGTTGGCATGCGCAAAGCCATTGATTTATATGCCTGTGTGCGGCCAGTCCAAACGCCACCGCTGGCCAACGC
CCGCGCTGGAGTCAATTTGGTGGTGGTACGCGAAAATACCGAGGGTTTGTATAGCGGCCAAGAAACCCGCGAGGGCGATG
AACGGGCTACGGCGCAACGAATTATCACCCGCCAAGCCAGCGAACGGATTGTGCAATGGGCAGTCCAATATGCCCAACGC
ACTGGCCGCCGCAAAATCACGGTGGTACACAAAGCCAATGTGCTACGCGAAACCTGTGGTTTGTTCCGCGAAACTGCACT
GCGCGTGCTAAGCGATGCGCCCGATTTACAAGTTGAAGAAATGTTAGTCGATAACGCTGCTTATCAATTGGCGCGTGCTC
CCGAGCGCTTTGAAGTGTTGGTCACCACCAATTTGTTTGGCGATATTCTCTCAGATGTAGCTAGCGTTTGGGGTGGCGGG
CTTGGTTTGGCAGCATCGGCCAATTATGGCACGCGCACGGCGGTATTCGAGCCTGTGCATGGTAGCGCACCGGATATTGC
CGGCCAAGGCATCGCCAATCCCTTGGCAACCTTGAGCGCTAGCGTGTTGATGCTCGAATTTGTGGGCTTGAACAGCTACG
CCGAGCGTTTGCAAACTGCGATTCAAGCGGTATTAGCCAATGGGCCATATACGCCCGATCTAGCTGGCGCGGCGACGACT
GCCGAAGTAGTGCAAGCCGTGATTGACCAATTTTGA

Upstream 100 bases:

>100_bases
TACCACCACCAGAAGGCTGGATGCGTGAAGTCTGGGCTGAAGGCGGAATCGTGCCATTCTACCGTCGCCATGGTCGCTTT
CCCGCGACAGGAGCCTAAAC

Downstream 100 bases:

>100_bases
TCCACGAAGCGCACGAAGGACACGAAGAGGGTACGAAACCGCGAAGGACGCGAAGAACACGAAAGGGCGAGGGTTCAGGG
GTCAGAGATCAGAGGTCAGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: HICDH; Isohomocitrate dehydrogenase; IHDH; NAD-dependent threo-isohomocitrate dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTRLCLIAGDGIGREVVQAARQVLEALAVPAEFVEAEAGWETFQRTGNALPEQTLAAVQAANSTLFGAVSSPSQRVAGYR
SPIVGMRKAIDLYACVRPVQTPPLANARAGVNLVVVRENTEGLYSGQETREGDERATAQRIITRQASERIVQWAVQYAQR
TGRRKITVVHKANVLRETCGLFRETALRVLSDAPDLQVEEMLVDNAAYQLARAPERFEVLVTTNLFGDILSDVASVWGGG
LGLAASANYGTRTAVFEPVHGSAPDIAGQGIANPLATLSASVLMLEFVGLNSYAERLQTAIQAVLANGPYTPDLAGAATT
AEVVQAVIDQF

Sequences:

>Translated_331_residues
MTRLCLIAGDGIGREVVQAARQVLEALAVPAEFVEAEAGWETFQRTGNALPEQTLAAVQAANSTLFGAVSSPSQRVAGYR
SPIVGMRKAIDLYACVRPVQTPPLANARAGVNLVVVRENTEGLYSGQETREGDERATAQRIITRQASERIVQWAVQYAQR
TGRRKITVVHKANVLRETCGLFRETALRVLSDAPDLQVEEMLVDNAAYQLARAPERFEVLVTTNLFGDILSDVASVWGGG
LGLAASANYGTRTAVFEPVHGSAPDIAGQGIANPLATLSASVLMLEFVGLNSYAERLQTAIQAVLANGPYTPDLAGAATT
AEVVQAVIDQF
>Mature_330_residues
TRLCLIAGDGIGREVVQAARQVLEALAVPAEFVEAEAGWETFQRTGNALPEQTLAAVQAANSTLFGAVSSPSQRVAGYRS
PIVGMRKAIDLYACVRPVQTPPLANARAGVNLVVVRENTEGLYSGQETREGDERATAQRIITRQASERIVQWAVQYAQRT
GRRKITVVHKANVLRETCGLFRETALRVLSDAPDLQVEEMLVDNAAYQLARAPERFEVLVTTNLFGDILSDVASVWGGGL
GLAASANYGTRTAVFEPVHGSAPDIAGQGIANPLATLSASVLMLEFVGLNSYAERLQTAIQAVLANGPYTPDLAGAATTA
EVVQAVIDQF

Specific function: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate), (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate, and 2-oxosuberate, respective

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=327, Percent_Identity=37.3088685015291, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI4758582, Length=332, Percent_Identity=35.8433734939759, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI28178838, Length=307, Percent_Identity=35.5048859934853, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI28178821, Length=331, Percent_Identity=32.6283987915408, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI28178816, Length=328, Percent_Identity=33.2317073170732, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI28178819, Length=196, Percent_Identity=35.2040816326531, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1788101, Length=353, Percent_Identity=36.2606232294618, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI87081683, Length=344, Percent_Identity=34.3023255813954, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=26.775956284153, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71986051, Length=339, Percent_Identity=36.5781710914454, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI25144293, Length=334, Percent_Identity=32.6347305389222, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17505779, Length=328, Percent_Identity=32.6219512195122, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=32.8402366863905, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322097, Length=345, Percent_Identity=42.6086956521739, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324709, Length=334, Percent_Identity=38.3233532934132, Blast_Score=219, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=34.7181008902077, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=31.830985915493, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=38.0239520958084, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=37.4251497005988, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24661184, Length=336, Percent_Identity=35.4166666666667, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI161078635, Length=330, Percent_Identity=31.2121212121212, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI161078633, Length=330, Percent_Identity=31.2121212121212, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24650122, Length=330, Percent_Identity=31.2121212121212, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI161078637, Length=330, Percent_Identity=31.2121212121212, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI161078639, Length=328, Percent_Identity=31.4024390243902, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI281362242, Length=332, Percent_Identity=34.6385542168675, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24648872, Length=332, Percent_Identity=34.6385542168675, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI20130355, Length=333, Percent_Identity=28.2282282282282, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45550436, Length=266, Percent_Identity=21.0526315789474, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.87 [H]

Molecular weight: Translated: 35320; Mature: 35189

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLCLIAGDGIGREVVQAARQVLEALAVPAEFVEAEAGWETFQRTGNALPEQTLAAVQA
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHH
ANSTLFGAVSSPSQRVAGYRSPIVGMRKAIDLYACVRPVQTPPLANARAGVNLVVVRENT
HCCCEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC
EGLYSGQETREGDERATAQRIITRQASERIVQWAVQYAQRTGRRKITVVHKANVLRETCG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
LFRETALRVLSDAPDLQVEEMLVDNAAYQLARAPERFEVLVTTNLFGDILSDVASVWGGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
LGLAASANYGTRTAVFEPVHGSAPDIAGQGIANPLATLSASVLMLEFVGLNSYAERLQTA
CCEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IQAVLANGPYTPDLAGAATTAEVVQAVIDQF
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRLCLIAGDGIGREVVQAARQVLEALAVPAEFVEAEAGWETFQRTGNALPEQTLAAVQA
CEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHH
ANSTLFGAVSSPSQRVAGYRSPIVGMRKAIDLYACVRPVQTPPLANARAGVNLVVVRENT
HCCCEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC
EGLYSGQETREGDERATAQRIITRQASERIVQWAVQYAQRTGRRKITVVHKANVLRETCG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
LFRETALRVLSDAPDLQVEEMLVDNAAYQLARAPERFEVLVTTNLFGDILSDVASVWGGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
LGLAASANYGTRTAVFEPVHGSAPDIAGQGIANPLATLSASVLMLEFVGLNSYAERLQTA
CCEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IQAVLANGPYTPDLAGAATTAEVVQAVIDQF
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]