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The map label for this gene is purB [H]

Identifier: 159899764

GI number: 159899764

Start: 4108634

End: 4110010

Strand: Direct

Name: purB [H]

Synonym: Haur_3246

Alternate gene names: 159899764

Gene position: 4108634-4110010 (Clockwise)

Preceding gene: 159899763

Following gene: 159899766

Centisome position: 64.74

GC content: 50.4

Gene sequence:

>1377_bases
ATGAGCGAGATTAATCAACAGGCTTTACTGGCGATTGGGCCGCTTGATGGCCGCTATCGTAAAGATGTTGAGGCGCTTAG
TGGCCATTTTAGCGAAGCCGCGTTATGGCGCTATCGCGTGCGAGTTGAAGTTGAATATTTATTATTTTTGGCGCGAGCAC
CACGCATCGGCCTGATCGAAGGCATCGATGGGCAGCTGGCCGGAGCTTTGCGCAATTTATATCGCCAATTTAGCGTCGAT
GATGCAGTGGCAATCGCAACCTGGGATCGTAAAGTTAACCACGATGTGAAGGCAGTTGAATATTGGCTGCGCGAAAAGCT
CGATCTGCTTGGGCTTGGCCAATACAAAGAGGCAGTGCACTTTGCCCTGACCTCGGAAGATGTCAATAACCTAGCCTATG
CCTTGATGGTGCAAGAATCGCGTGATGTGGTGTTATTGCCAGCCTTGGGCAATATTCTCGGCCTCTTGCGTGATATGCAA
ATTGAGCATGCCGAAGCACCCTTATTAGCTAAAACCCACGGCCAACCAGCCACCCCCACCACCATGGGCAAAGAAATTGC
GGTTTTCGCCGCCCGTGTCAATCGGGGCATCGAACAGCTTTTACACATTCGATTAACTGGCAAACTAAATGGGGCAACTG
GCACATATGCTGCCCATTATGCTGCTTTTCCTGATGTTGATTGGATTACATTTAGCTCGGCCTTTGTGAAGTTTTTGGGC
TTGGAGCCAACCCTGCTGAGCACCCAAATTGAGCCACACGATAGCATTGTTGAGGTTGGCGATGCCTTGCGCCGCGTCAA
CACAATTCTGGTGGATCTCTCACAGGATATGTGGCGCTATATTTCCGATTCGGTGTTTGGCCAAGCGAGTGTAGCGGGCG
AAGTTGGTTCATCGACCATGCCGCACAAAGTTAACCCAATTGACTTTGAAAATACCGAAGGCAATTTATTGCTAGCCAAT
AGCTTGCTGGAGTTTTTTGGCCGCAAGTTGCCAATTTCGCGACTGCAACGCGATTTATCGGATAGCACGGTGTTGCGCAA
TCTTGGCGTGGCTTTTGGCCATAGTTTGTTTGCCTATCAACGTTTGCTCAAGGGCTTGCGCAAATTGGCCTTCAATACCG
AAAAAGCCTACGCTGAATTAGCCGGGCATCCCGAAGTTCTGACCGAGGCAATTCAAACGATTTTGCGCCGCGAACGCTAC
CCAGAGCCATACGAAGCCATGAAAAAGCTGGCTCGTGGTCGCCAAATTACGCTTGAAGATTTGCATAGCTTTATCGATGA
GCTTGAGGTGCGGCCCGAAGTGCGCGAGGAATTACGGGCTTTGCGGCCTGAAACTTATATCGGCCTCGCCGCCAAACTGG
CCCGCAAGAAGATTTAA

Upstream 100 bases:

>100_bases
CCCGATCCCCAAGCCCTACTTTTGATTTTTGCCCTCTAACTTTTGACTTTTGATCTACAATGGAGTGTATTTAATTCTAC
CAACCATAGTGAGGATTCTC

Downstream 100 bases:

>100_bases
TGGCGCTTTAGCCCCGAATAGTACATTATTCGGGGCTTATTTGAGCCGCAAACAAAGCAGCACCAATAATTCCCGCATCG
TTGGTAAGCCGAGCAGGCAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSEINQQALLAIGPLDGRYRKDVEALSGHFSEAALWRYRVRVEVEYLLFLARAPRIGLIEGIDGQLAGALRNLYRQFSVD
DAVAIATWDRKVNHDVKAVEYWLREKLDLLGLGQYKEAVHFALTSEDVNNLAYALMVQESRDVVLLPALGNILGLLRDMQ
IEHAEAPLLAKTHGQPATPTTMGKEIAVFAARVNRGIEQLLHIRLTGKLNGATGTYAAHYAAFPDVDWITFSSAFVKFLG
LEPTLLSTQIEPHDSIVEVGDALRRVNTILVDLSQDMWRYISDSVFGQASVAGEVGSSTMPHKVNPIDFENTEGNLLLAN
SLLEFFGRKLPISRLQRDLSDSTVLRNLGVAFGHSLFAYQRLLKGLRKLAFNTEKAYAELAGHPEVLTEAIQTILRRERY
PEPYEAMKKLARGRQITLEDLHSFIDELEVRPEVREELRALRPETYIGLAAKLARKKI

Sequences:

>Translated_458_residues
MSEINQQALLAIGPLDGRYRKDVEALSGHFSEAALWRYRVRVEVEYLLFLARAPRIGLIEGIDGQLAGALRNLYRQFSVD
DAVAIATWDRKVNHDVKAVEYWLREKLDLLGLGQYKEAVHFALTSEDVNNLAYALMVQESRDVVLLPALGNILGLLRDMQ
IEHAEAPLLAKTHGQPATPTTMGKEIAVFAARVNRGIEQLLHIRLTGKLNGATGTYAAHYAAFPDVDWITFSSAFVKFLG
LEPTLLSTQIEPHDSIVEVGDALRRVNTILVDLSQDMWRYISDSVFGQASVAGEVGSSTMPHKVNPIDFENTEGNLLLAN
SLLEFFGRKLPISRLQRDLSDSTVLRNLGVAFGHSLFAYQRLLKGLRKLAFNTEKAYAELAGHPEVLTEAIQTILRRERY
PEPYEAMKKLARGRQITLEDLHSFIDELEVRPEVREELRALRPETYIGLAAKLARKKI
>Mature_457_residues
SEINQQALLAIGPLDGRYRKDVEALSGHFSEAALWRYRVRVEVEYLLFLARAPRIGLIEGIDGQLAGALRNLYRQFSVDD
AVAIATWDRKVNHDVKAVEYWLREKLDLLGLGQYKEAVHFALTSEDVNNLAYALMVQESRDVVLLPALGNILGLLRDMQI
EHAEAPLLAKTHGQPATPTTMGKEIAVFAARVNRGIEQLLHIRLTGKLNGATGTYAAHYAAFPDVDWITFSSAFVKFLGL
EPTLLSTQIEPHDSIVEVGDALRRVNTILVDLSQDMWRYISDSVFGQASVAGEVGSSTMPHKVNPIDFENTEGNLLLANS
LLEFFGRKLPISRLQRDLSDSTVLRNLGVAFGHSLFAYQRLLKGLRKLAFNTEKAYAELAGHPEVLTEAIQTILRRERYP
EPYEAMKKLARGRQITLEDLHSFIDELEVRPEVREELRALRPETYIGLAAKLARKKI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=447, Percent_Identity=48.5458612975391, Blast_Score=430, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51380; Mature: 51249

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEINQQALLAIGPLDGRYRKDVEALSGHFSEAALWRYRVRVEVEYLLFLARAPRIGLIE
CCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHEEEEHHHHHHHHCCCCCCEEE
GIDGQLAGALRNLYRQFSVDDAVAIATWDRKVNHDVKAVEYWLREKLDLLGLGQYKEAVH
CCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
FALTSEDVNNLAYALMVQESRDVVLLPALGNILGLLRDMQIEHAEAPLLAKTHGQPATPT
HEECHHHHHHHEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
TMGKEIAVFAARVNRGIEQLLHIRLTGKLNGATGTYAAHYAAFPDVDWITFSSAFVKFLG
CCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHC
LEPTLLSTQIEPHDSIVEVGDALRRVNTILVDLSQDMWRYISDSVFGQASVAGEVGSSTM
CCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
PHKVNPIDFENTEGNLLLANSLLEFFGRKLPISRLQRDLSDSTVLRNLGVAFGHSLFAYQ
CCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RLLKGLRKLAFNTEKAYAELAGHPEVLTEAIQTILRRERYPEPYEAMKKLARGRQITLED
HHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHH
LHSFIDELEVRPEVREELRALRPETYIGLAAKLARKKI
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEINQQALLAIGPLDGRYRKDVEALSGHFSEAALWRYRVRVEVEYLLFLARAPRIGLIE
CCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHEEEEHHHHHHHHCCCCCCEEE
GIDGQLAGALRNLYRQFSVDDAVAIATWDRKVNHDVKAVEYWLREKLDLLGLGQYKEAVH
CCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
FALTSEDVNNLAYALMVQESRDVVLLPALGNILGLLRDMQIEHAEAPLLAKTHGQPATPT
HEECHHHHHHHEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
TMGKEIAVFAARVNRGIEQLLHIRLTGKLNGATGTYAAHYAAFPDVDWITFSSAFVKFLG
CCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHC
LEPTLLSTQIEPHDSIVEVGDALRRVNTILVDLSQDMWRYISDSVFGQASVAGEVGSSTM
CCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
PHKVNPIDFENTEGNLLLANSLLEFFGRKLPISRLQRDLSDSTVLRNLGVAFGHSLFAYQ
CCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RLLKGLRKLAFNTEKAYAELAGHPEVLTEAIQTILRRERYPEPYEAMKKLARGRQITLED
HHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHH
LHSFIDELEVRPEVREELRALRPETYIGLAAKLARKKI
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]