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The map label for this gene is lasT [H]

Identifier: 159899747

GI number: 159899747

Start: 4088288

End: 4089013

Strand: Direct

Name: lasT [H]

Synonym: Haur_3229

Alternate gene names: 159899747

Gene position: 4088288-4089013 (Clockwise)

Preceding gene: 159899746

Following gene: 159899748

Centisome position: 64.42

GC content: 53.99

Gene sequence:

>726_bases
GTGAATTCGACAATTTTTGCCAATATCATCACGGTGCTCCACGAACCACAGCGCGAGGTCAATATTGGCTCGACAGTGCG
GGCCTTGCTCAATACAGGCTTTCAGCGCTTGCGCCTAGTTCAGCCAGTCGCCTACTCAATTGAGCGGCTTGACGATATGG
CGCATCGCAGTGGCCCATTGGTTGAGCAAATTGAAACATTTGCTAGCCTTGGCGAGGCCTTAGCCAATGTGAGCTATGTG
GTTGGGTTTAGCGGGCGCAGTCGCGGCGAGCGCCAAGCGGTTAGTTTGCCCGAATTTGCGCCAACTTTGACCGCGCTGGC
AGCAACCGCTCCGGTAGCCTTCGTATTTGGCCGCGAAGATCGTGGGCTGGATAACGCCGCAATCGAACAATGCAGTCAAT
TAATCAGTATTCCGGTCGATCCAAGCTATCCTTCGTTGAATTTGGCCGATGCGGTGTTGTTGGCGTTGTATACAATTCGC
GGCTTGGCTACGAGCGCCGAGCCATTGCCTAGCCCTGATTTGGCCGACCAAGCTTCCCACGAGTTGCTGCTGAATTTGCT
TGATCAACTTTTGACCAAGCTTAACTTTTCACGCACGGCAGGCCAACAAGCGAGTGCAGTGCGGGTTATGCGTGATCTGC
TGGCTCGTTCAAGGCCTAGCCCCAGCGAAATTGCCCTCGTAACCGCAATCTGTCGCAAAATGTTGGCTGCACTGCCAACA
GCCTAA

Upstream 100 bases:

>100_bases
AACCGCCATATAACGAGCTGAGAGGTTTAGATAGGTATGTTACCTTCGGATTTTCTTTCGGTGCTATCATACCAAACGGT
TTTGTTTCAGAGGATTTGTA

Downstream 100 bases:

>100_bases
TTCCCGACACATATCCAACTTTCTGTTTAAGTGGAGATTGCTGTTGCCAGCCATAAATTGAAAGGGGTGCAGGGGGATGA
AAACACCCTGCGTTTCCTTC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNSTIFANIITVLHEPQREVNIGSTVRALLNTGFQRLRLVQPVAYSIERLDDMAHRSGPLVEQIETFASLGEALANVSYV
VGFSGRSRGERQAVSLPEFAPTLTALAATAPVAFVFGREDRGLDNAAIEQCSQLISIPVDPSYPSLNLADAVLLALYTIR
GLATSAEPLPSPDLADQASHELLLNLLDQLLTKLNFSRTAGQQASAVRVMRDLLARSRPSPSEIALVTAICRKMLAALPT
A

Sequences:

>Translated_241_residues
MNSTIFANIITVLHEPQREVNIGSTVRALLNTGFQRLRLVQPVAYSIERLDDMAHRSGPLVEQIETFASLGEALANVSYV
VGFSGRSRGERQAVSLPEFAPTLTALAATAPVAFVFGREDRGLDNAAIEQCSQLISIPVDPSYPSLNLADAVLLALYTIR
GLATSAEPLPSPDLADQASHELLLNLLDQLLTKLNFSRTAGQQASAVRVMRDLLARSRPSPSEIALVTAICRKMLAALPT
A
>Mature_241_residues
MNSTIFANIITVLHEPQREVNIGSTVRALLNTGFQRLRLVQPVAYSIERLDDMAHRSGPLVEQIETFASLGEALANVSYV
VGFSGRSRGERQAVSLPEFAPTLTALAATAPVAFVFGREDRGLDNAAIEQCSQLISIPVDPSYPSLNLADAVLLALYTIR
GLATSAEPLPSPDLADQASHELLLNLLDQLLTKLNFSRTAGQQASAVRVMRDLLARSRPSPSEIALVTAICRKMLAALPT
A

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=176, Percent_Identity=35.2272727272727, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788881, Length=240, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25931; Mature: 25931

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTIFANIITVLHEPQREVNIGSTVRALLNTGFQRLRLVQPVAYSIERLDDMAHRSGPL
CCCHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VEQIETFASLGEALANVSYVVGFSGRSRGERQAVSLPEFAPTLTALAATAPVAFVFGRED
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHEEECCCC
RGLDNAAIEQCSQLISIPVDPSYPSLNLADAVLLALYTIRGLATSAEPLPSPDLADQASH
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
ELLLNLLDQLLTKLNFSRTAGQQASAVRVMRDLLARSRPSPSEIALVTAICRKMLAALPT
HHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
A
C
>Mature Secondary Structure
MNSTIFANIITVLHEPQREVNIGSTVRALLNTGFQRLRLVQPVAYSIERLDDMAHRSGPL
CCCHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VEQIETFASLGEALANVSYVVGFSGRSRGERQAVSLPEFAPTLTALAATAPVAFVFGRED
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHEEECCCC
RGLDNAAIEQCSQLISIPVDPSYPSLNLADAVLLALYTIRGLATSAEPLPSPDLADQASH
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
ELLLNLLDQLLTKLNFSRTAGQQASAVRVMRDLLARSRPSPSEIALVTAICRKMLAALPT
HHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]