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The map label for this gene is rpfG [H]

Identifier: 159899737

GI number: 159899737

Start: 4071225

End: 4073606

Strand: Direct

Name: rpfG [H]

Synonym: Haur_3219

Alternate gene names: 159899737

Gene position: 4071225-4073606 (Clockwise)

Preceding gene: 159899726

Following gene: 159899742

Centisome position: 64.15

GC content: 48.66

Gene sequence:

>2382_bases
ATGCACTGGCGCTCGTTTATTCCACGGACACTAGCCGATTCCCAATCTCGTCAACAGTTATTGATTGTCATGCTCAAAAG
CGTTGTTTGGATCTTTACTCCCGTCCAAGTTATCTTGAGTTTTACCTTGACAACCACAAGCTGGCCACGCCTGTTGTTGC
TGGCGATTGTCAACCTGCTTTGTGCTGGAATCTGGCTCTTAATCAAACGCAATAAGCTGGATCTGGCGGGCAAGCTGTTT
GTTGGGTTGTTTTGGTTGTTATTTACGGGTTTGATGCTGAGCACTGGTGGCATTAGCTCGCCTTCGATTATTGCCTACTT
TTTCGTCATTTTTACCGCCAGCTTTTTGTTGGGAGAACTGGCAAGTGTGATTATCGGCTGTCTGAGTTTAGCGGCAACCT
TTGTAGCCGTGCTCCTTGAGCTGAATCAGCTTTTGCCCGCCTCGGTGATTGTTTATACCCCCGTTTCGCGTTGGCTCAGT
TACAGCTTTTATCTTGGCATTATGCTGATATTTCAGATGGTCAGTGGGCGCTTGGTCTATAAGGCCATGCAAAAAGCCCA
AGCCGAGTTACACGAACGCCAACGCATCGAAGCTGAATTACGCCATTCTGAAGCCCAATATCGTCTTTTGTTCGATACTA
TTCCAATTGGCATTGGCATGGCTAAACTCGATGGTACAGTCATCGCCATCAACCCAGCTGGCAGCCAAATGATGGGCTAT
AGCCATGCCGAATTGATGCAAATCAAACTCGAACAGTTATATGCCAATCCCGATCAACGGGCTAGTTTAATTCAGCAAGC
CCAGCAAACTGGCAAGATTCGCGACCGCGAGATGGCTTTCCGGCGCAACGATGGCCAATTGGTTTGGGCGTTGGTCAATC
TTGATCTTGGGTTAATTAATGGTGAGGCGGTGACGATTGCCACGCTGCGCGATAACACCACTCAACGCGCAGCAGAACAA
GCCTTGCGCACCAACGAAGCTCGTTTTCGGGCAATCTTTGAGCATGCAGCAATCGGAATTGTCTTAATTGATAATAATGG
TGTGGTCTTTAGCGCCAACCCAACTGCTTGTATGTTGCTTAATTTCAGTGAACATGAATTGCAACAACAAGCCTTTGTTA
ATTTCACCCATCCCGATGATCGCCAATTTGAAATGAGCTTAAATCAAGAACTTCGCGCCGGATTATGTGATTCGTATCAA
ATTGAGCAGCGCTTTATTCGCTCTGATGGTGGGATTGTTTGGGGCCGTTTGTGTGCCTCATTAGTCCAAGATGCTGCTGA
TCAACCTTTATTTATGATTGCGATGATTGAAGATCTTAGCTCGTATAAAGATACCCAGGCCCAACTTGACTTGCAAATGC
AAACTCTGACAGCGCTCTACTACAGCTCACAACGCCTAACAACCAAACTCAAAGTCGAGGAATTGGCCCGCGATGGGGCC
GATAGTTGTGTTAGCATTTTTGGCGCACAACGGGCTTGGCTCACGCTCAATCAGGCTGATCAGCTTAACTATCACGAACA
TAACCTAGAACAACCCTTGCCCAGTGTTCACATTGAGATTGAATCGACTCAACCCATCACCCGCGATGGCCCAACTCGAA
CCATGGCCTTTCCGTTGGTCAGCCATAATCACACCTTTGGGATGCTCAATTTGCAGAGCAATCAGGCTGATTTCTTTCAA
GCTGAGCGCAACGATATGCTACAAACCTATGCCAGTCAGGTCGCCGCCGCCTTGGATAACGCGCTCATGTTCCAACATTT
GCAACAAATCAACCGTGAAGTTACCAGCGCCTACGATATGACGATTGAGGGTTGGTCTCGCGCCTTAGATTTGCGTGATC
ACGAGACTGAAGGCCATACCCAACGTGTTACCTGGATGACTGAACGGCTGGCCGCCGCAATGGGCCAATTTAGCGCCGAA
GAGTTGATCCATGTGCGACGAGGCGCTTTGCTCCACGATATTGGAAAAATGGGTGTGCCCGATGCCATCTTGCACAAGCC
TGGGCCGCTCAACGATGAAGAATGGGTGATTATGCGGCGGCATCCGGTCTATGCGTATCAACTGCTTGCGCCAATTGGCT
ACTTGCAAGGCTCGCTGGATATTCCGCATTATCATCATGAACGCTGGGATGGGGGCGGCTATCCCAAAGGCTTGCAGGCC
GAAGAAATTCCTTTGGCGGCGCGAGTATTTGCCGTAGTTGATGTATGGGATGCCTTGCGTTCTGATCGACCATATCGCAA
AGGTTGGTCAGATCAGCGGATTATGGACTATTTGGCGGGTGAGGCGGGCAAGCATTTTGATCCATTAGTCGTTGAAGTAT
TTTTGCAATTGCTCATGACCATGAGCATTGCCGAGGTGCGTAATCCAGTTAATGAAGCCTAA

Upstream 100 bases:

>100_bases
CAAGCCTATAGTATACTTAACCCACTACGTTTTGCGAATACCCTAGCCGCATTGCTGTCCAACGTTGCCTAACCACGCTA
GCCTATCTATGGTGAGAGTT

Downstream 100 bases:

>100_bases
AAAGCAACAGGCGGGCTAGCAAGCCAGCCCACCTTTGCAAGAGTTTATTGGAATTACCAAGGCTTACGTACCCTCACCCC
CGACCCCTCTCCCACTGCGG

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MHWRSFIPRTLADSQSRQQLLIVMLKSVVWIFTPVQVILSFTLTTTSWPRLLLLAIVNLLCAGIWLLIKRNKLDLAGKLF
VGLFWLLFTGLMLSTGGISSPSIIAYFFVIFTASFLLGELASVIIGCLSLAATFVAVLLELNQLLPASVIVYTPVSRWLS
YSFYLGIMLIFQMVSGRLVYKAMQKAQAELHERQRIEAELRHSEAQYRLLFDTIPIGIGMAKLDGTVIAINPAGSQMMGY
SHAELMQIKLEQLYANPDQRASLIQQAQQTGKIRDREMAFRRNDGQLVWALVNLDLGLINGEAVTIATLRDNTTQRAAEQ
ALRTNEARFRAIFEHAAIGIVLIDNNGVVFSANPTACMLLNFSEHELQQQAFVNFTHPDDRQFEMSLNQELRAGLCDSYQ
IEQRFIRSDGGIVWGRLCASLVQDAADQPLFMIAMIEDLSSYKDTQAQLDLQMQTLTALYYSSQRLTTKLKVEELARDGA
DSCVSIFGAQRAWLTLNQADQLNYHEHNLEQPLPSVHIEIESTQPITRDGPTRTMAFPLVSHNHTFGMLNLQSNQADFFQ
AERNDMLQTYASQVAAALDNALMFQHLQQINREVTSAYDMTIEGWSRALDLRDHETEGHTQRVTWMTERLAAAMGQFSAE
ELIHVRRGALLHDIGKMGVPDAILHKPGPLNDEEWVIMRRHPVYAYQLLAPIGYLQGSLDIPHYHHERWDGGGYPKGLQA
EEIPLAARVFAVVDVWDALRSDRPYRKGWSDQRIMDYLAGEAGKHFDPLVVEVFLQLLMTMSIAEVRNPVNEA

Sequences:

>Translated_793_residues
MHWRSFIPRTLADSQSRQQLLIVMLKSVVWIFTPVQVILSFTLTTTSWPRLLLLAIVNLLCAGIWLLIKRNKLDLAGKLF
VGLFWLLFTGLMLSTGGISSPSIIAYFFVIFTASFLLGELASVIIGCLSLAATFVAVLLELNQLLPASVIVYTPVSRWLS
YSFYLGIMLIFQMVSGRLVYKAMQKAQAELHERQRIEAELRHSEAQYRLLFDTIPIGIGMAKLDGTVIAINPAGSQMMGY
SHAELMQIKLEQLYANPDQRASLIQQAQQTGKIRDREMAFRRNDGQLVWALVNLDLGLINGEAVTIATLRDNTTQRAAEQ
ALRTNEARFRAIFEHAAIGIVLIDNNGVVFSANPTACMLLNFSEHELQQQAFVNFTHPDDRQFEMSLNQELRAGLCDSYQ
IEQRFIRSDGGIVWGRLCASLVQDAADQPLFMIAMIEDLSSYKDTQAQLDLQMQTLTALYYSSQRLTTKLKVEELARDGA
DSCVSIFGAQRAWLTLNQADQLNYHEHNLEQPLPSVHIEIESTQPITRDGPTRTMAFPLVSHNHTFGMLNLQSNQADFFQ
AERNDMLQTYASQVAAALDNALMFQHLQQINREVTSAYDMTIEGWSRALDLRDHETEGHTQRVTWMTERLAAAMGQFSAE
ELIHVRRGALLHDIGKMGVPDAILHKPGPLNDEEWVIMRRHPVYAYQLLAPIGYLQGSLDIPHYHHERWDGGGYPKGLQA
EEIPLAARVFAVVDVWDALRSDRPYRKGWSDQRIMDYLAGEAGKHFDPLVVEVFLQLLMTMSIAEVRNPVNEA
>Mature_793_residues
MHWRSFIPRTLADSQSRQQLLIVMLKSVVWIFTPVQVILSFTLTTTSWPRLLLLAIVNLLCAGIWLLIKRNKLDLAGKLF
VGLFWLLFTGLMLSTGGISSPSIIAYFFVIFTASFLLGELASVIIGCLSLAATFVAVLLELNQLLPASVIVYTPVSRWLS
YSFYLGIMLIFQMVSGRLVYKAMQKAQAELHERQRIEAELRHSEAQYRLLFDTIPIGIGMAKLDGTVIAINPAGSQMMGY
SHAELMQIKLEQLYANPDQRASLIQQAQQTGKIRDREMAFRRNDGQLVWALVNLDLGLINGEAVTIATLRDNTTQRAAEQ
ALRTNEARFRAIFEHAAIGIVLIDNNGVVFSANPTACMLLNFSEHELQQQAFVNFTHPDDRQFEMSLNQELRAGLCDSYQ
IEQRFIRSDGGIVWGRLCASLVQDAADQPLFMIAMIEDLSSYKDTQAQLDLQMQTLTALYYSSQRLTTKLKVEELARDGA
DSCVSIFGAQRAWLTLNQADQLNYHEHNLEQPLPSVHIEIESTQPITRDGPTRTMAFPLVSHNHTFGMLNLQSNQADFFQ
AERNDMLQTYASQVAAALDNALMFQHLQQINREVTSAYDMTIEGWSRALDLRDHETEGHTQRVTWMTERLAAAMGQFSAE
ELIHVRRGALLHDIGKMGVPDAILHKPGPLNDEEWVIMRRHPVYAYQLLAPIGYLQGSLDIPHYHHERWDGGGYPKGLQA
EEIPLAARVFAVVDVWDALRSDRPYRKGWSDQRIMDYLAGEAGKHFDPLVVEVFLQLLMTMSIAEVRNPVNEA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=131, Percent_Identity=32.0610687022901, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 89538; Mature: 89538

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWRSFIPRTLADSQSRQQLLIVMLKSVVWIFTPVQVILSFTLTTTSWPRLLLLAIVNLL
CCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CAGIWLLIKRNKLDLAGKLFVGLFWLLFTGLMLSTGGISSPSIIAYFFVIFTASFLLGEL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ASVIIGCLSLAATFVAVLLELNQLLPASVIVYTPVSRWLSYSFYLGIMLIFQMVSGRLVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
KAMQKAQAELHERQRIEAELRHSEAQYRLLFDTIPIGIGMAKLDGTVIAINPAGSQMMGY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCEEEEECCEEEEECCCCCCCCCC
SHAELMQIKLEQLYANPDQRASLIQQAQQTGKIRDREMAFRRNDGQLVWALVNLDLGLIN
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCEEEEC
GEAVTIATLRDNTTQRAAEQALRTNEARFRAIFEHAAIGIVLIDNNGVVFSANPTACMLL
CCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEECCCCEEEECCCCEEEEE
NFSEHELQQQAFVNFTHPDDRQFEMSLNQELRAGLCDSYQIEQRFIRSDGGIVWGRLCAS
ECHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEHHHHHHH
LVQDAADQPLFMIAMIEDLSSYKDTQAQLDLQMQTLTALYYSSQRLTTKLKVEELARDGA
HHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
DSCVSIFGAQRAWLTLNQADQLNYHEHNLEQPLPSVHIEIESTQPITRDGPTRTMAFPLV
HHHHHHHCCCCCEEEECCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEEE
SHNHTFGMLNLQSNQADFFQAERNDMLQTYASQVAAALDNALMFQHLQQINREVTSAYDM
ECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TIEGWSRALDLRDHETEGHTQRVTWMTERLAAAMGQFSAEELIHVRRGALLHDIGKMGVP
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
DAILHKPGPLNDEEWVIMRRHPVYAYQLLAPIGYLQGSLDIPHYHHERWDGGGYPKGLQA
HHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
EEIPLAARVFAVVDVWDALRSDRPYRKGWSDQRIMDYLAGEAGKHFDPLVVEVFLQLLMT
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
MSIAEVRNPVNEA
HHHHHHHCCCCCC
>Mature Secondary Structure
MHWRSFIPRTLADSQSRQQLLIVMLKSVVWIFTPVQVILSFTLTTTSWPRLLLLAIVNLL
CCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CAGIWLLIKRNKLDLAGKLFVGLFWLLFTGLMLSTGGISSPSIIAYFFVIFTASFLLGEL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ASVIIGCLSLAATFVAVLLELNQLLPASVIVYTPVSRWLSYSFYLGIMLIFQMVSGRLVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
KAMQKAQAELHERQRIEAELRHSEAQYRLLFDTIPIGIGMAKLDGTVIAINPAGSQMMGY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCEEEEECCEEEEECCCCCCCCCC
SHAELMQIKLEQLYANPDQRASLIQQAQQTGKIRDREMAFRRNDGQLVWALVNLDLGLIN
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCEEEEC
GEAVTIATLRDNTTQRAAEQALRTNEARFRAIFEHAAIGIVLIDNNGVVFSANPTACMLL
CCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEECCCCEEEECCCCEEEEE
NFSEHELQQQAFVNFTHPDDRQFEMSLNQELRAGLCDSYQIEQRFIRSDGGIVWGRLCAS
ECHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEHHHHHHH
LVQDAADQPLFMIAMIEDLSSYKDTQAQLDLQMQTLTALYYSSQRLTTKLKVEELARDGA
HHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
DSCVSIFGAQRAWLTLNQADQLNYHEHNLEQPLPSVHIEIESTQPITRDGPTRTMAFPLV
HHHHHHHCCCCCEEEECCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEEE
SHNHTFGMLNLQSNQADFFQAERNDMLQTYASQVAAALDNALMFQHLQQINREVTSAYDM
ECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TIEGWSRALDLRDHETEGHTQRVTWMTERLAAAMGQFSAEELIHVRRGALLHDIGKMGVP
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
DAILHKPGPLNDEEWVIMRRHPVYAYQLLAPIGYLQGSLDIPHYHHERWDGGGYPKGLQA
HHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
EEIPLAARVFAVVDVWDALRSDRPYRKGWSDQRIMDYLAGEAGKHFDPLVVEVFLQLLMT
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
MSIAEVRNPVNEA
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]