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The map label for this gene is trmD

Identifier: 159899501

GI number: 159899501

Start: 3765532

End: 3766296

Strand: Direct

Name: trmD

Synonym: Haur_2982

Alternate gene names: 159899501

Gene position: 3765532-3766296 (Clockwise)

Preceding gene: 159899497

Following gene: 159899509

Centisome position: 59.33

GC content: 53.73

Gene sequence:

>765_bases
ATGCGTTTTGATATTTTGACCTTGTTTCCGGCCATGTTTACTGGCCCGCTGACCGAAAGCATTTTGAAACGGGCGGCTCA
GGCTGAATTATTGCAATTTCACTTGCATGATATTCGCGATTACGCCACCGACAAGCATAAGATGGTCGATGATTCGCCAA
CTGGCGGCGGGGCAGGGATGGTGATGAAGCCTGGGCCGTTGGCGCTTTGCACTCAGACAGTACTGTCCGCCGATCCCAAT
CCAGCTCCGGTGGTGCTGATGACTCCCAGCGGACGTGTGTTTAACCAAACGATCGCCCGTGAATGGGCGCAATTGCCGCG
TTTAGTGCTGGTGTGTGGTCATTACGAGGGCATCGATGAGCGCTATATCGAACGTTATGTGACCGATCAAGTGTCGTTGG
GCGATTTTGTACTGACTGGCGGTGAGCTGGCAGCAATGACTATTGTCGATGCAGTTGGCCGCCTCGTGCCCGAAGTGCTC
GACCCTGAATCGCTGCTGCACGAAAGCCACGACGATGGCCTATTGGAATATCCACATTACACCAAGCCCGCCGAATGGAA
TGGGGTTGCCGTGCCGCCAATTTTGCTGAGTGGGCATCACGCCAAGATTGCCCAATGGCGACATGAGCAGCGTTTACGCC
GCACCTTGGAGCGCCGCCCCGATATGTTGCGCCATGCCACGCTCTCCAAAAAAGATCGTCAGTTACTCAAAGAATGGGGC
TGGGAAGAAAAATCAGAAGGCAAAAATCAAAAGGCAGAAGGCTAA

Upstream 100 bases:

>100_bases
ACAGCGATTTTTTGCACCAGCCCTAAGCCATCGAGTATCATAGGCGTTGATTAACAAACAACAATCAACAGGTTAAATCT
GCTACGATAGGATTTTTGCA

Downstream 100 bases:

>100_bases
GATCTAAGAAGAGATAGCTATGAGCTATCGGAATTAATGAGCTACCACAAAAACGTTCCGATAGCCCATAGCTTTCATCC
TTACAATCCTTCGTGCGCTT

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRFDILTLFPAMFTGPLTESILKRAAQAELLQFHLHDIRDYATDKHKMVDDSPTGGGAGMVMKPGPLALCTQTVLSADPN
PAPVVLMTPSGRVFNQTIAREWAQLPRLVLVCGHYEGIDERYIERYVTDQVSLGDFVLTGGELAAMTIVDAVGRLVPEVL
DPESLLHESHDDGLLEYPHYTKPAEWNGVAVPPILLSGHHAKIAQWRHEQRLRRTLERRPDMLRHATLSKKDRQLLKEWG
WEEKSEGKNQKAEG

Sequences:

>Translated_254_residues
MRFDILTLFPAMFTGPLTESILKRAAQAELLQFHLHDIRDYATDKHKMVDDSPTGGGAGMVMKPGPLALCTQTVLSADPN
PAPVVLMTPSGRVFNQTIAREWAQLPRLVLVCGHYEGIDERYIERYVTDQVSLGDFVLTGGELAAMTIVDAVGRLVPEVL
DPESLLHESHDDGLLEYPHYTKPAEWNGVAVPPILLSGHHAKIAQWRHEQRLRRTLERRPDMLRHATLSKKDRQLLKEWG
WEEKSEGKNQKAEG
>Mature_254_residues
MRFDILTLFPAMFTGPLTESILKRAAQAELLQFHLHDIRDYATDKHKMVDDSPTGGGAGMVMKPGPLALCTQTVLSADPN
PAPVVLMTPSGRVFNQTIAREWAQLPRLVLVCGHYEGIDERYIERYVTDQVSLGDFVLTGGELAAMTIVDAVGRLVPEVL
DPESLLHESHDDGLLEYPHYTKPAEWNGVAVPPILLSGHHAKIAQWRHEQRLRRTLERRPDMLRHATLSKKDRQLLKEWG
WEEKSEGKNQKAEG

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=247, Percent_Identity=42.1052631578947, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_HERA2 (A9B3P8)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001545748.1
- ProteinModelPortal:   A9B3P8
- SMR:   A9B3P8
- GeneID:   5734854
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_2982
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- BioCyc:   HAUR316274:HAUR_2982-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28557; Mature: 28557

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDILTLFPAMFTGPLTESILKRAAQAELLQFHLHDIRDYATDKHKMVDDSPTGGGAGM
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCE
VMKPGPLALCTQTVLSADPNPAPVVLMTPSGRVFNQTIAREWAQLPRLVLVCGHYEGIDE
EECCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEHCCCCCCHHH
RYIERYVTDQVSLGDFVLTGGELAAMTIVDAVGRLVPEVLDPESLLHESHDDGLLEYPHY
HHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEECCCC
TKPAEWNGVAVPPILLSGHHAKIAQWRHEQRLRRTLERRPDMLRHATLSKKDRQLLKEWG
CCCCCCCCEECCCCEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHCC
WEEKSEGKNQKAEG
CCCCCCCCCCCCCC
>Mature Secondary Structure
MRFDILTLFPAMFTGPLTESILKRAAQAELLQFHLHDIRDYATDKHKMVDDSPTGGGAGM
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCE
VMKPGPLALCTQTVLSADPNPAPVVLMTPSGRVFNQTIAREWAQLPRLVLVCGHYEGIDE
EECCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEHCCCCCCHHH
RYIERYVTDQVSLGDFVLTGGELAAMTIVDAVGRLVPEVLDPESLLHESHDDGLLEYPHY
HHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEECCCC
TKPAEWNGVAVPPILLSGHHAKIAQWRHEQRLRRTLERRPDMLRHATLSKKDRQLLKEWG
CCCCCCCCEECCCCEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHCC
WEEKSEGKNQKAEG
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA