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The map label for this gene is dagK [H]

Identifier: 159898086

GI number: 159898086

Start: 1813805

End: 1814698

Strand: Reverse

Name: dagK [H]

Synonym: Haur_1562

Alternate gene names: 159898086

Gene position: 1814698-1813805 (Counterclockwise)

Preceding gene: 159898087

Following gene: 159898085

Centisome position: 28.59

GC content: 53.24

Gene sequence:

>894_bases
ATGAAACGTGTAACAGTCATTCTTAATCCAAATGCCGGCAATGCCCACCAACGCCGCGCCATCGCCCAAGGCATCACTGA
ATGGCGCAGCAACCAAGGCTGGCAAGTGCGGTTACGCGAAACTCGCAAGGCTGGCGATGCTACCAGTTTTGCCCGCGAAG
AAGCCAAACGCAACGATTTAATTGTGGCGGCTGGCGGCGATGGCACGATCAACGAGGTCATGAATGGCCTCGTTGGCACT
GATACCGCGCTGGGAGCCTTGCCAGTTGGCACAGGCAATGTGTGGGTTCGCGAACTACAACAATCGCTCAATCCATTGCA
TGCAGCTCGCCAATTGGCTGATGGCCATGTTGAGTTGGTTGATGTTGGCCAAGCTAATGAGCGCTATTTTTTGCTGATGG
CAGGCGTTGGCTTAGATGCAGCAATTACCCGCGAAGTCCATTCGGCTGATAAAAAACGCTTAGGCCGCTTGGCCTATGTG
ATCAAAAGCTTGCCTGTGCTTTGGCGCTTGCGAGGCACGCGCACCCGCATTAGCCTTGATGGCCAGCCGCTCAAGGGCAA
TGCCCTGTTTGTGTTAATTAGCAACTCACGGCTGTATGGTGGTGTGTTGAATATTGCCTATCGGGCAGCCATGCGCGATG
GTATGCTCGATGTTTGTACCATGATGGGCGATAGTGCCCTTGATGCCCCTAAATTGCTAGCTGGAATTTTGTTCCGTGGC
TATGGCGTGATCCAAGGCTTAGAGTATGTGCAAGCTCGTGAGATCGAGATTGCCTGCTCCAAGCCTTTGCCAATTCAAGT
CGATGGCGATGCGATTGGCACAACACCCATGACCTTTCGGGTGGTTCCGCAAACGCTGCGCGTACTCTTACCGCGTACCA
CTGAAATCAACTAA

Upstream 100 bases:

>100_bases
GTGGCTGGATCGAAACGCCAGCAACCTTGCTTGAAAGTATCGAACGTCGCGGAGCACAGGCAGCACTGAATTTTAATAAT
GATGATGATAACGACGACGA

Downstream 100 bases:

>100_bases
CCCTATAGCCAGATTGAGGTTCTGTGTGAGACGTTCGTTCTATTTGTTGATCCTACTGTGTTTGTTAGCCGCCTGTAGCG
AAGCAGGCTCAGCCCTGCCA

Product: diacylglycerol kinase catalytic protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKRVTVILNPNAGNAHQRRAIAQGITEWRSNQGWQVRLRETRKAGDATSFAREEAKRNDLIVAAGGDGTINEVMNGLVGT
DTALGALPVGTGNVWVRELQQSLNPLHAARQLADGHVELVDVGQANERYFLLMAGVGLDAAITREVHSADKKRLGRLAYV
IKSLPVLWRLRGTRTRISLDGQPLKGNALFVLISNSRLYGGVLNIAYRAAMRDGMLDVCTMMGDSALDAPKLLAGILFRG
YGVIQGLEYVQAREIEIACSKPLPIQVDGDAIGTTPMTFRVVPQTLRVLLPRTTEIN

Sequences:

>Translated_297_residues
MKRVTVILNPNAGNAHQRRAIAQGITEWRSNQGWQVRLRETRKAGDATSFAREEAKRNDLIVAAGGDGTINEVMNGLVGT
DTALGALPVGTGNVWVRELQQSLNPLHAARQLADGHVELVDVGQANERYFLLMAGVGLDAAITREVHSADKKRLGRLAYV
IKSLPVLWRLRGTRTRISLDGQPLKGNALFVLISNSRLYGGVLNIAYRAAMRDGMLDVCTMMGDSALDAPKLLAGILFRG
YGVIQGLEYVQAREIEIACSKPLPIQVDGDAIGTTPMTFRVVPQTLRVLLPRTTEIN
>Mature_297_residues
MKRVTVILNPNAGNAHQRRAIAQGITEWRSNQGWQVRLRETRKAGDATSFAREEAKRNDLIVAAGGDGTINEVMNGLVGT
DTALGALPVGTGNVWVRELQQSLNPLHAARQLADGHVELVDVGQANERYFLLMAGVGLDAAITREVHSADKKRLGRLAYV
IKSLPVLWRLRGTRTRISLDGQPLKGNALFVLISNSRLYGGVLNIAYRAAMRDGMLDVCTMMGDSALDAPKLLAGILFRG
YGVIQGLEYVQAREIEIACSKPLPIQVDGDAIGTTPMTFRVVPQTLRVLLPRTTEIN

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=270, Percent_Identity=30, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32358; Mature: 32358

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVTVILNPNAGNAHQRRAIAQGITEWRSNQGWQVRLRETRKAGDATSFAREEAKRNDL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCHHHHHHHHHCCCCE
IVAAGGDGTINEVMNGLVGTDTALGALPVGTGNVWVRELQQSLNPLHAARQLADGHVELV
EEEECCCCCHHHHHCCCCCCCCHHCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DVGQANERYFLLMAGVGLDAAITREVHSADKKRLGRLAYVIKSLPVLWRLRGTRTRISLD
ECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEC
GQPLKGNALFVLISNSRLYGGVLNIAYRAAMRDGMLDVCTMMGDSALDAPKLLAGILFRG
CCCCCCCEEEEEEECCEEHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHH
YGVIQGLEYVQAREIEIACSKPLPIQVDGDAIGTTPMTFRVVPQTLRVLLPRTTEIN
HHHHHHHHHHHHEEEEEEECCCCCEEECCCCCCCCCEEEEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRVTVILNPNAGNAHQRRAIAQGITEWRSNQGWQVRLRETRKAGDATSFAREEAKRNDL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCHHHHHHHHHCCCCE
IVAAGGDGTINEVMNGLVGTDTALGALPVGTGNVWVRELQQSLNPLHAARQLADGHVELV
EEEECCCCCHHHHHCCCCCCCCHHCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DVGQANERYFLLMAGVGLDAAITREVHSADKKRLGRLAYVIKSLPVLWRLRGTRTRISLD
ECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEC
GQPLKGNALFVLISNSRLYGGVLNIAYRAAMRDGMLDVCTMMGDSALDAPKLLAGILFRG
CCCCCCCEEEEEEECCEEHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHH
YGVIQGLEYVQAREIEIACSKPLPIQVDGDAIGTTPMTFRVVPQTLRVLLPRTTEIN
HHHHHHHHHHHHEEEEEEECCCCCEEECCCCCCCCCEEEEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]