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The map label for this gene is ams [H]

Identifier: 159897682

GI number: 159897682

Start: 1323927

End: 1325900

Strand: Direct

Name: ams [H]

Synonym: Haur_1153

Alternate gene names: 159897682

Gene position: 1323927-1325900 (Clockwise)

Preceding gene: 159897681

Following gene: 159897683

Centisome position: 20.86

GC content: 50.51

Gene sequence:

>1974_bases
ATGCAACCACTCACAGTTCAAACTATGCATTTGCCCAATCCAACCACGTTCGATGATCTGCTCGATCAACAGATTGCCAA
TTCGCGTGATCGCGATATTTTTCGCTTGCGCATGCAACGCCATTTTGGCGATTGTTTAGAAGCGCTAGGAGCGTTGTATG
CCCAGCATCCAGCTTGGCCACAGTTGTTGGAGCAATTGCCCGAACGCTTGATTACTGCCTATGCCCAGCGCCGCGATGCC
CTGAAAATTCACGATTTAGCCCGCGAAATCCAGCCCGATTGGTTTGCTGAGGCCACCATGGTTGGCGGCATTTACTATGT
TGATCGCTTGGCAGGCACATTGCGCGGGGTGATTGAGCATATTGATTATTTGCAAGAATTGGGTTTGACCTATGTGCATC
TGATGCCGCTATTACAGCCACGCCATGGCCCCAACGATGGCGGCTATGCGGTGCTCGATTATCGCTCGATTGATCAACGG
CTTGGCAATGTGGCCGATTTTATCGAATTAAGCGATTTGCTCCGTACCAACGGCATCAGCTTATGCATTGATGTGGTGGT
GAATCACACGGCCAAAGAGCATGAATGGGCAGTCAAGGCCCGTGCTGGTGATGCCCAATATTTGGATTACTATCTGAGTT
TTGCCGATCGCAGTTTGCCTGATGCCTATGAGCAACATTTACCCGAAGTGTTTCCCGATTTTGCGCCTGGTAATTTTACT
TGGTATGCCGAGTTGAGCGAGCATGGCCGTTGGGTTTGGACGACCTTCAACGAATTTCAATGGGATTTGAACTATACCAA
CCCCATGGTTTGGCTGGAGATGCTGGATATTTTGCTGTATCTCGCCAATCTAGGCGTTGATGTGCTGCGTTTGGATGCCG
TGCCGTTTATGTGGAAACGCCTCGGCACGAATTGCCAAAATCAGCCCGAAGTGCTCGATTTGTTACAAGCTTGGCGAGCA
GCCATGCGGATCGTCTGTCCGGCGACAATTTTCAAGGCCGAGGCGATTGTTGCCCCCGACGATTTGGTGCAATATTTGGG
TTTGGGACGGCGCACAGGCAAGCTCTGTGAAATTGCCTACCATAATTCGCTGATGGTGTTGTTGTGGAGTGCCTTGGCCT
CGCAACGCGCCGATCTGTTTACGCAATCGCTGTTGAACATGCCTGCAACGCCCAGCAATGCCGCTTGGATTACCTATGTG
CGCTGCCACGATGATATTGGCTGGGCTGTGACCGACCACAATGCAGCTTTGGTTGGCGAAGATGGGCCATTGCATCGCCA
ATTTTTAAGCGCTTGGTATAGTGGCGAATTTGCTGGTAGTTTTGCGCGGGGCGAGGTGTTTCAATATAATCCACTCACCA
ACGATCGCCGAATTAGCGGCATGACTGCCTCGTTGGCTGGGCTAGAGCAAGCCTTGGAAACCACCGATCCAGCAGCGATT
GAATTGACAATTCGCCGGATTGCGTTGCTGTATGCCGTGATTTTTAGCTTTGGTGGCATTCCGTTGATCTATATGGGCGA
TGAATTGGGCATGCTCAATGATCACAGCTACTTGCATGACCCTACCAAAGCCAACGATAACCGCTGGTTGCATCGCCCAG
CCATGGATTGGTGCTTAGCGGCCCAACGCCATGATCCAACTACGCTTGCTGGGCGCTTATGGCAGGTATTGCGCCATTTG
ATTCAGGTGCGCCAACATACTCCAGCCTTGCATAGCGCAGGCCAAACCTTGCCAATCTGGACACAGCAACGCCATGTTTT
AGGGGTGGTTCGAGTTCACCCATTGGGGCGAATTTTAATTCTTGGAAACCTTTCCGCCACCCCACAGCGGGTCAGTTTAG
CGGTTATTCAACAAGCAGGGCTGGTTGGTCGCTTATATAATTTGTTGGATAACGATTCACTTAATATCGATACACAAAGC
CATGAAATTATACTCGATGCATATCAATGTTGTTGGCTCAGCATTCAAGCCTAA

Upstream 100 bases:

>100_bases
GGTTAAAACCCTTGGAAGGCCGAGGGCACGGCGATTTGATTTCATTTAGTTAATCCAACATTACAGCTTAAAAACCATCG
GCAGTATGAGGAGTGTGGTT

Downstream 100 bases:

>100_bases
ACTTTTTCAAATCAAAAAATAAGCTTCCGATAACGATTGCAGTCCTGAATTGGATCAAGTATAGTTGCGCTATCCCTGTT
CTTTTTTCAGAAGGAGCGCA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MQPLTVQTMHLPNPTTFDDLLDQQIANSRDRDIFRLRMQRHFGDCLEALGALYAQHPAWPQLLEQLPERLITAYAQRRDA
LKIHDLAREIQPDWFAEATMVGGIYYVDRLAGTLRGVIEHIDYLQELGLTYVHLMPLLQPRHGPNDGGYAVLDYRSIDQR
LGNVADFIELSDLLRTNGISLCIDVVVNHTAKEHEWAVKARAGDAQYLDYYLSFADRSLPDAYEQHLPEVFPDFAPGNFT
WYAELSEHGRWVWTTFNEFQWDLNYTNPMVWLEMLDILLYLANLGVDVLRLDAVPFMWKRLGTNCQNQPEVLDLLQAWRA
AMRIVCPATIFKAEAIVAPDDLVQYLGLGRRTGKLCEIAYHNSLMVLLWSALASQRADLFTQSLLNMPATPSNAAWITYV
RCHDDIGWAVTDHNAALVGEDGPLHRQFLSAWYSGEFAGSFARGEVFQYNPLTNDRRISGMTASLAGLEQALETTDPAAI
ELTIRRIALLYAVIFSFGGIPLIYMGDELGMLNDHSYLHDPTKANDNRWLHRPAMDWCLAAQRHDPTTLAGRLWQVLRHL
IQVRQHTPALHSAGQTLPIWTQQRHVLGVVRVHPLGRILILGNLSATPQRVSLAVIQQAGLVGRLYNLLDNDSLNIDTQS
HEIILDAYQCCWLSIQA

Sequences:

>Translated_657_residues
MQPLTVQTMHLPNPTTFDDLLDQQIANSRDRDIFRLRMQRHFGDCLEALGALYAQHPAWPQLLEQLPERLITAYAQRRDA
LKIHDLAREIQPDWFAEATMVGGIYYVDRLAGTLRGVIEHIDYLQELGLTYVHLMPLLQPRHGPNDGGYAVLDYRSIDQR
LGNVADFIELSDLLRTNGISLCIDVVVNHTAKEHEWAVKARAGDAQYLDYYLSFADRSLPDAYEQHLPEVFPDFAPGNFT
WYAELSEHGRWVWTTFNEFQWDLNYTNPMVWLEMLDILLYLANLGVDVLRLDAVPFMWKRLGTNCQNQPEVLDLLQAWRA
AMRIVCPATIFKAEAIVAPDDLVQYLGLGRRTGKLCEIAYHNSLMVLLWSALASQRADLFTQSLLNMPATPSNAAWITYV
RCHDDIGWAVTDHNAALVGEDGPLHRQFLSAWYSGEFAGSFARGEVFQYNPLTNDRRISGMTASLAGLEQALETTDPAAI
ELTIRRIALLYAVIFSFGGIPLIYMGDELGMLNDHSYLHDPTKANDNRWLHRPAMDWCLAAQRHDPTTLAGRLWQVLRHL
IQVRQHTPALHSAGQTLPIWTQQRHVLGVVRVHPLGRILILGNLSATPQRVSLAVIQQAGLVGRLYNLLDNDSLNIDTQS
HEIILDAYQCCWLSIQA
>Mature_657_residues
MQPLTVQTMHLPNPTTFDDLLDQQIANSRDRDIFRLRMQRHFGDCLEALGALYAQHPAWPQLLEQLPERLITAYAQRRDA
LKIHDLAREIQPDWFAEATMVGGIYYVDRLAGTLRGVIEHIDYLQELGLTYVHLMPLLQPRHGPNDGGYAVLDYRSIDQR
LGNVADFIELSDLLRTNGISLCIDVVVNHTAKEHEWAVKARAGDAQYLDYYLSFADRSLPDAYEQHLPEVFPDFAPGNFT
WYAELSEHGRWVWTTFNEFQWDLNYTNPMVWLEMLDILLYLANLGVDVLRLDAVPFMWKRLGTNCQNQPEVLDLLQAWRA
AMRIVCPATIFKAEAIVAPDDLVQYLGLGRRTGKLCEIAYHNSLMVLLWSALASQRADLFTQSLLNMPATPSNAAWITYV
RCHDDIGWAVTDHNAALVGEDGPLHRQFLSAWYSGEFAGSFARGEVFQYNPLTNDRRISGMTASLAGLEQALETTDPAAI
ELTIRRIALLYAVIFSFGGIPLIYMGDELGMLNDHSYLHDPTKANDNRWLHRPAMDWCLAAQRHDPTTLAGRLWQVLRHL
IQVRQHTPALHSAGQTLPIWTQQRHVLGVVRVHPLGRILILGNLSATPQRVSLAVIQQAGLVGRLYNLLDNDSLNIDTQS
HEIILDAYQCCWLSIQA

Specific function: Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose s

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=212, Percent_Identity=27.8301886792453, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI87081873, Length=529, Percent_Identity=24.952741020794, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790687, Length=217, Percent_Identity=32.258064516129, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583749, Length=290, Percent_Identity=27.9310344827586, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24583747, Length=290, Percent_Identity=27.9310344827586, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45549022, Length=285, Percent_Identity=25.9649122807018, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24586591, Length=186, Percent_Identity=30.6451612903226, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586593, Length=341, Percent_Identity=25.2199413489736, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24586587, Length=281, Percent_Identity=26.3345195729537, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24586589, Length=310, Percent_Identity=26.4516129032258, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330053, Length=198, Percent_Identity=30.8080808080808, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24586599, Length=276, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583745, Length=288, Percent_Identity=25, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24586597, Length=290, Percent_Identity=25.8620689655172, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281360393, Length=307, Percent_Identity=24.1042345276873, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.4 [H]

Molecular weight: Translated: 74570; Mature: 74570

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLTVQTMHLPNPTTFDDLLDQQIANSRDRDIFRLRMQRHFGDCLEALGALYAQHPAWP
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLLEQLPERLITAYAQRRDALKIHDLAREIQPDWFAEATMVGGIYYVDRLAGTLRGVIEH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDYLQELGLTYVHLMPLLQPRHGPNDGGYAVLDYRSIDQRLGNVADFIELSDLLRTNGIS
HHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHH
LCIDVVVNHTAKEHEWAVKARAGDAQYLDYYLSFADRSLPDAYEQHLPEVFPDFAPGNFT
HHHHHHHHCCCHHCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCHHHCCCCCCCCEE
WYAELSEHGRWVWTTFNEFQWDLNYTNPMVWLEMLDILLYLANLGVDVLRLDAVPFMWKR
EEEEHHHCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
LGTNCQNQPEVLDLLQAWRAAMRIVCPATIFKAEAIVAPDDLVQYLGLGRRTGKLCEIAY
HCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCEEECHHHHHHHHCCCCCCCCEEEEEE
HNSLMVLLWSALASQRADLFTQSLLNMPATPSNAAWITYVRCHDDIGWAVTDHNAALVGE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEECCCEEEECC
DGPLHRQFLSAWYSGEFAGSFARGEVFQYNPLTNDRRISGMTASLAGLEQALETTDPAAI
CCCHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
ELTIRRIALLYAVIFSFGGIPLIYMGDELGMLNDHSYLHDPTKANDNRWLHRPAMDWCLA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHH
AQRHDPTTLAGRLWQVLRHLIQVRQHTPALHSAGQTLPIWTQQRHVLGVVRVHPLGRILI
HCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHEEEEEECCCCEEEE
LGNLSATPQRVSLAVIQQAGLVGRLYNLLDNDSLNIDTQSHEIILDAYQCCWLSIQA
EECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHEEHHHHEEEECC
>Mature Secondary Structure
MQPLTVQTMHLPNPTTFDDLLDQQIANSRDRDIFRLRMQRHFGDCLEALGALYAQHPAWP
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLLEQLPERLITAYAQRRDALKIHDLAREIQPDWFAEATMVGGIYYVDRLAGTLRGVIEH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDYLQELGLTYVHLMPLLQPRHGPNDGGYAVLDYRSIDQRLGNVADFIELSDLLRTNGIS
HHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHH
LCIDVVVNHTAKEHEWAVKARAGDAQYLDYYLSFADRSLPDAYEQHLPEVFPDFAPGNFT
HHHHHHHHCCCHHCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCHHHCCCCCCCCEE
WYAELSEHGRWVWTTFNEFQWDLNYTNPMVWLEMLDILLYLANLGVDVLRLDAVPFMWKR
EEEEHHHCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
LGTNCQNQPEVLDLLQAWRAAMRIVCPATIFKAEAIVAPDDLVQYLGLGRRTGKLCEIAY
HCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCEEECHHHHHHHHCCCCCCCCEEEEEE
HNSLMVLLWSALASQRADLFTQSLLNMPATPSNAAWITYVRCHDDIGWAVTDHNAALVGE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEECCCEEEECC
DGPLHRQFLSAWYSGEFAGSFARGEVFQYNPLTNDRRISGMTASLAGLEQALETTDPAAI
CCCHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
ELTIRRIALLYAVIFSFGGIPLIYMGDELGMLNDHSYLHDPTKANDNRWLHRPAMDWCLA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHH
AQRHDPTTLAGRLWQVLRHLIQVRQHTPALHSAGQTLPIWTQQRHVLGVVRVHPLGRILI
HCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHEEEEEECCCCEEEE
LGNLSATPQRVSLAVIQQAGLVGRLYNLLDNDSLNIDTQSHEIILDAYQCCWLSIQA
EECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHEEHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12517860 [H]