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The map label for this gene is miaB

Identifier: 159897576

GI number: 159897576

Start: 1194894

End: 1196210

Strand: Reverse

Name: miaB

Synonym: Haur_1047

Alternate gene names: 159897576

Gene position: 1196210-1194894 (Counterclockwise)

Preceding gene: 159897577

Following gene: 159897575

Centisome position: 18.85

GC content: 47.53

Gene sequence:

>1317_bases
ATGGAACGAAATCGCTATTTTGTATGGACAGTTGGCTGCCAAATGAACGTCTCTGATTCAGAGCGACTTGAATCGGCACT
GCAAGGAGTAGGCTATACTCCTGCGGAGCAAGCTGAAGATGCCGATTTTATTGTATTAAATTCGTGCTCAGTGCGAGCCA
ATGCTGAAGAAAAGATTATCGGCAAAATCACCGATATTCAACGGATCAAACGAGAGCGGCCTGATACCAAAATTGTGTTA
TGGGGCTGTATGGTCGGCCCAAATAATCAATCGATCTTCAAGAAAAAATTGCCAATGGTTGATCATTTTGTCTCGCCATC
GGCAGTTGATGAGGTTTTGGCTTTAGCGCCTAACCCAATCTACCAACTTGATGAACCAGCCTTGCCTGTAGCCGATTGGC
AAGTGCCGCCAGTTAATGTTCATGTACCAATTAATTATGGCTGCAATATGTCGTGTGCCTATTGTGTGATTCCGTTGCGC
CGTGGTAAAGAGCGTTCGCGCCCAATGGAAGAAATTGCCGAAGAAGTGCGGCGAATTTGTGCCCGTGGAGCCAAAGAAAT
TACCTTGCTGGGCCAAATTGTCGATTCATATGGCCACGATTTGCCAGGGCGGCCTGACCTTGCTGATTTGCTGGAATATT
TGCATGAAACCCCAGGTTTAGTACGCCTACGCTTTTTGACCTCGCACCCAGCCTTTATGAGCGAAAAATTGTTGCATACA
ATTGCCCGCTTGCCCAAAGTTATGCCTGATATTAATTTGCCAATTCAAGCAGGCGACGATCAATTGCTCAAGGTGATGAA
GCGTGGTTACACTGTGGCTAAATATACCAAATTGATCGAACGGATTCGCGAGATTATCCCCAACGTGTCATTATCAACTG
ATATTATTGTTGGGCATCCGGGCGAAACTCGCGAGATGTTTGAGCGCACCTTGGAGATGGTTGAAAACATTCGGTTTGAT
AAAGTGCATATTGCCGCCTACTCCTCGCGCCCAGGCACCAAAGCTGCTGATATGGAGCTTGACCCAGCCTTGGCAGTTGA
ACATGGTGAAAAGCAATATCGGCGGATTGCACTTGAACGATTGCAAGAACGGATTGCCACTGAGCGCAACGAAGAATGCT
TGGGGCATGAGGTTGAGGTTTTGGTCGAGGAATTCACCAAAGGCAAATGGCGTGGCCGTGATCGCAACAACAAATTGGTC
TTTTTCGAGGCCGACGGCGATTGGTATGGCAAAGTGGTTAACATTCACGTCACAGAAACTCGCCCATGGTGGTTGGGTGG
CAACCTGATCGGCGAAACGCTAGCAATAGGTGCATAA

Upstream 100 bases:

>100_bases
ACGATTGGCTTGATCTCACGCTTGCGACGGTTGCGATGGCCTGCTTGATAGGCTATAATGCTGCGACTTTTGCTATTAAC
GACATAAAGGTTGTAGGGTT

Downstream 100 bases:

>100_bases
ATGAGTTATGGCAATGATGATTCACAACGTGAACGCAATGATGCAGTTTTGAAAGCACTCGGTGGTGTACCGCAACCGCC
AGTTGCTCCGTCTGTGCAAC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MERNRYFVWTVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEKIIGKITDIQRIKRERPDTKIVL
WGCMVGPNNQSIFKKKLPMVDHFVSPSAVDEVLALAPNPIYQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLR
RGKERSRPMEEIAEEVRRICARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPAFMSEKLLHT
IARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSLSTDIIVGHPGETREMFERTLEMVENIRFD
KVHIAAYSSRPGTKAADMELDPALAVEHGEKQYRRIALERLQERIATERNEECLGHEVEVLVEEFTKGKWRGRDRNNKLV
FFEADGDWYGKVVNIHVTETRPWWLGGNLIGETLAIGA

Sequences:

>Translated_438_residues
MERNRYFVWTVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEKIIGKITDIQRIKRERPDTKIVL
WGCMVGPNNQSIFKKKLPMVDHFVSPSAVDEVLALAPNPIYQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLR
RGKERSRPMEEIAEEVRRICARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPAFMSEKLLHT
IARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSLSTDIIVGHPGETREMFERTLEMVENIRFD
KVHIAAYSSRPGTKAADMELDPALAVEHGEKQYRRIALERLQERIATERNEECLGHEVEVLVEEFTKGKWRGRDRNNKLV
FFEADGDWYGKVVNIHVTETRPWWLGGNLIGETLAIGA
>Mature_438_residues
MERNRYFVWTVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEKIIGKITDIQRIKRERPDTKIVL
WGCMVGPNNQSIFKKKLPMVDHFVSPSAVDEVLALAPNPIYQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLR
RGKERSRPMEEIAEEVRRICARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPAFMSEKLLHT
IARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSLSTDIIVGHPGETREMFERTLEMVENIRFD
KVHIAAYSSRPGTKAADMELDPALAVEHGEKQYRRIALERLQERIATERNEECLGHEVEVLVEEFTKGKWRGRDRNNKLV
FFEADGDWYGKVVNIHVTETRPWWLGGNLIGETLAIGA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=449, Percent_Identity=32.0712694877506, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI28872782, Length=451, Percent_Identity=31.9290465631929, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=27.6169265033408, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=33.6322869955157, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1787057, Length=399, Percent_Identity=27.8195488721804, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=30.5084745762712, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71996771, Length=261, Percent_Identity=28.735632183908, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21356207, Length=429, Percent_Identity=31.4685314685315, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI19922432, Length=448, Percent_Identity=27.6785714285714, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_HERA2 (A9AZY8)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001543823.1
- ProteinModelPortal:   A9AZY8
- SMR:   A9AZY8
- GeneID:   5732951
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_1047
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- ProtClustDB:   PRK14338
- BioCyc:   HAUR316274:HAUR_1047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR000385
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49687; Mature: 49687

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNRYFVWTVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEKII
CCCCEEEEEEECEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHH
GKITDIQRIKRERPDTKIVLWGCMVGPNNQSIFKKKLPMVDHFVSPSAVDEVLALAPNPI
HHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCC
YQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLRRGKERSRPMEEIAEEVRRIC
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
ARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPAFMSEKLLHT
HCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
IARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSLSTDIIVGHP
HHHHHHHCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
GETREMFERTLEMVENIRFDKVHIAAYSSRPGTKAADMELDPALAVEHGEKQYRRIALER
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
LQERIATERNEECLGHEVEVLVEEFTKGKWRGRDRNNKLVFFEADGDWYGKVVNIHVTET
HHHHHHHCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEEEECC
RPWWLGGNLIGETLAIGA
CCEEECCHHHHHHHHCCC
>Mature Secondary Structure
MERNRYFVWTVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEKII
CCCCEEEEEEECEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHH
GKITDIQRIKRERPDTKIVLWGCMVGPNNQSIFKKKLPMVDHFVSPSAVDEVLALAPNPI
HHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCC
YQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLRRGKERSRPMEEIAEEVRRIC
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
ARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPAFMSEKLLHT
HCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
IARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSLSTDIIVGHP
HHHHHHHCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
GETREMFERTLEMVENIRFDKVHIAAYSSRPGTKAADMELDPALAVEHGEKQYRRIALER
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
LQERIATERNEECLGHEVEVLVEEFTKGKWRGRDRNNKLVFFEADGDWYGKVVNIHVTET
HHHHHHHCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEEEECC
RPWWLGGNLIGETLAIGA
CCEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA