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The map label for this gene is hph [H]

Identifier: 159897553

GI number: 159897553

Start: 1168367

End: 1169290

Strand: Reverse

Name: hph [H]

Synonym: Haur_1024

Alternate gene names: 159897553

Gene position: 1169290-1168367 (Counterclockwise)

Preceding gene: 159897556

Following gene: 159897552

Centisome position: 18.42

GC content: 45.24

Gene sequence:

>924_bases
ATGCAGATTAATCCAGCATTAGCGCAGGCAATTGAGGCCGATTCAGGCTTTGAATTGACCCAAGTGGAGTTTATCGGAGC
AGGTTGGTTTGCCCAAGCCTATCGATTTTGCAGCCAAGCCAAGCATTATGTAGTGCGGATTAGCAAGCATTACCACGATT
TTTTAAAGGATGTCTACGCCTACCAGCATTGGGGCCAACAACTACCAATTCCAGCAATTCTGGCGCATGGTCAATTTGCT
GATGGCTGGGCCTACGCGATTAGCCCACATATTGCAGGCCAAACAATTGTCTCACTCGAAGCAGCAGAACTCAGGCAAAT
TCAGCCAGCACTCTTTAAAAGTTTGCAGCAACTGCATCAACTTAAGCTAGGACCAGTCACCGGCTGGGGTTCGGTCAACA
GTCAAGGCCATGGTCGCTATCAATCATGGGCTGAAGAAGTTTTAGATATTGGCAATTACAAATTTGATTTCGATTGGCAT
GATTGGATCAAACGTGATGATCAAACAGGGACGTTGCTGCGGCAAGGTTATCGGCTAATGGAGCAATTGTTGAGCACAAT
CAGCACTGAACGATCTTTAATTCATCGTGATTTTGGCTTTGATAATGTGCTGTGTCAGCCACCAACAATTGTGGCCGTGC
TCGATTGGGCCGATTTTGGCTATGGCGATTGGGTTTATGATATTGCCCAGCAAAGCTGTTATGGTCAAGCTGAAATCTAT
TTACAGCCATGGCTCAAATTCGCCCAACAACACGATTTAATTCCTGATAATTTTGAAAAACGTTTGCACTGCTATTGGCT
GCGCATGAATTTGACCGATTTGATTGTCTCACAGTTGCGCAACGATTGGCATTGGCAGCGTAAAATTGTGGCAGAATTGA
GCTGGTTGATTGAGAATCCTGTGGTAGTGAGGTTGAATCAATGA

Upstream 100 bases:

>100_bases
AATCTGTGGCAAAATAAAACCTTCGTGGGCTTCGTGTCCTTCGTGGATCAATCGGGGTACAATCAACGTCGAGCACTATT
CAATTATTGGAGAACGTTTG

Downstream 100 bases:

>100_bases
AGATCACCACAAGTCAAGAGCTGATCGGCTGGCATCGGTATCTTGAGCAATTGGGTCCACGGCTGACTGGCAGTGAGGCT
CATCAGGCCTTTATCGAATT

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: APH(4); Hygromycin B phosphotransferase; Hygromycin-B kinase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHYHDFLKDVYAYQHWGQQLPIPAILAHGQFA
DGWAYAISPHIAGQTIVSLEAAELRQIQPALFKSLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWH
DWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLIHRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIY
LQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ

Sequences:

>Translated_307_residues
MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHYHDFLKDVYAYQHWGQQLPIPAILAHGQFA
DGWAYAISPHIAGQTIVSLEAAELRQIQPALFKSLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWH
DWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLIHRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIY
LQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ
>Mature_307_residues
MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHYHDFLKDVYAYQHWGQQLPIPAILAHGQFA
DGWAYAISPHIAGQTIVSLEAAELRQIQPALFKSLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWH
DWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLIHRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIY
LQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.163 [H]

Molecular weight: Translated: 35707; Mature: 35707

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHYHDFLKDVYA
CCCCHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQHWGQQLPIPAILAHGQFADGWAYAISPHIAGQTIVSLEAAELRQIQPALFKSLQQLHQ
HHHCCCCCCHHHHEECCCCCCCEEEEECCCCCCCHHEEECHHHHHHHHHHHHHHHHHHHH
LKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWHDWIKRDDQTGTLLRQGYRLM
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHHHHHHH
EQLLSTISTERSLIHRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIY
HHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHH
LQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENP
HHHHHHHHHHCCCCCCCHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVVRLNQ
EEEEECC
>Mature Secondary Structure
MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHYHDFLKDVYA
CCCCHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQHWGQQLPIPAILAHGQFADGWAYAISPHIAGQTIVSLEAAELRQIQPALFKSLQQLHQ
HHHCCCCCCHHHHEECCCCCCCEEEEECCCCCCCHHEEECHHHHHHHHHHHHHHHHHHHH
LKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWHDWIKRDDQTGTLLRQGYRLM
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHHHHHHH
EQLLSTISTERSLIHRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIY
HHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHH
LQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENP
HHHHHHHHHHCCCCCCCHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVVRLNQ
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6314265; 6319235; 6318050 [H]