Click here to switch to the map view.

The map label for this gene is lipA [H]

Identifier: 159897422

GI number: 159897422

Start: 1021059

End: 1021955

Strand: Direct

Name: lipA [H]

Synonym: Haur_0893

Alternate gene names: 159897422

Gene position: 1021059-1021955 (Clockwise)

Preceding gene: 159897412

Following gene: 159897427

Centisome position: 16.09

GC content: 54.07

Gene sequence:

>897_bases
ATGGATACAATTACACTCTTTGAAGCGCCCAAAGCTGCACCGGTGGCCCCGCGCCGCCCCGCATGGCTCAAAGCTCGCGC
TCCGATAGGGGAAAACTACGAGGATGTGCATGGCCTCATGCGCGAACTCGATTTGCATACGGTCTGCGAAGAAGCCCATT
GCCCTAATATTGGCGAATGTTGGAATCATCGCACTGCGACTTTTTTACTCCTCGGCGATACCTGTACCCGCGGTTGTCGC
TATTGCGCGATTGGCAAAGGCAAGCCGAAACCAATTGACGAACAAGAACCACAGCGCATCGCCGCTGCGGTCGCTCACCT
CAAACTCAAACATGTGGTGCTAACCTCGGTTAATCGCGATGATCAAGCTGATGGCGGAGCGCATATTTTTGCCGAATCGA
TTGACTTGATTCGTCAGCAATTGCCCGAATGTAAAATCGAGGTGCTGATTCCCGATTTTGATGGCAATTGGGAAGCCTTG
CGGATTGTGATGGATGCTCGTCCCGATGTGTTGAATCACAATATCGAAACCGTGCCACGCTTGTTCCGCAAATTCCGGCC
ACGGGCGAACTTCTACCAAAGCCTCGAATTATTGCGTTTGGCTCGCGACCTTCAGCCGAGCATTGTAACCAAAAGTGGCT
TGATGGTTGGTGCTGGCGAAACCGATGAAGAGGTGCTGACGGTGATGGATGCCCTTCGCCGATCCGATGTGGATGTGATG
ACAATTGGCCAATATCTCTCGCCATCGGGCGATCACTGGCCGATTGATCGGTATGTGCCACCCGAAACCTTTACATGGTT
CCGCGAGCAAGGCTTAGCGCGTGGTTTCCGCCACGTCGAATCCGGCCCGATGGTGCGCTCGTCGTATCACGCCCACGAGC
ACGTTGCAGGGTTGTAG

Upstream 100 bases:

>100_bases
TCGGGGTTGGGCCAAAAATCGGGTGCATGACAAGCAGCAAAAGCACGTTACAATATGAGCAAGAGCAAATGCTGCTCGTT
GCGTTATCCAAGGGGTTTTC

Downstream 100 bases:

>100_bases
GCGTTGGGGGTTGGGGATCGGTTGTTGGGGATCGGGTATTAATACCTGATCCCCATTCTTATGCCACAAAGAACAGGCAG
AATGGTTTAGTCGTTATTCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDTITLFEAPKAAPVAPRRPAWLKARAPIGENYEDVHGLMRELDLHTVCEEAHCPNIGECWNHRTATFLLLGDTCTRGCR
YCAIGKGKPKPIDEQEPQRIAAAVAHLKLKHVVLTSVNRDDQADGGAHIFAESIDLIRQQLPECKIEVLIPDFDGNWEAL
RIVMDARPDVLNHNIETVPRLFRKFRPRANFYQSLELLRLARDLQPSIVTKSGLMVGAGETDEEVLTVMDALRRSDVDVM
TIGQYLSPSGDHWPIDRYVPPETFTWFREQGLARGFRHVESGPMVRSSYHAHEHVAGL

Sequences:

>Translated_298_residues
MDTITLFEAPKAAPVAPRRPAWLKARAPIGENYEDVHGLMRELDLHTVCEEAHCPNIGECWNHRTATFLLLGDTCTRGCR
YCAIGKGKPKPIDEQEPQRIAAAVAHLKLKHVVLTSVNRDDQADGGAHIFAESIDLIRQQLPECKIEVLIPDFDGNWEAL
RIVMDARPDVLNHNIETVPRLFRKFRPRANFYQSLELLRLARDLQPSIVTKSGLMVGAGETDEEVLTVMDALRRSDVDVM
TIGQYLSPSGDHWPIDRYVPPETFTWFREQGLARGFRHVESGPMVRSSYHAHEHVAGL
>Mature_298_residues
MDTITLFEAPKAAPVAPRRPAWLKARAPIGENYEDVHGLMRELDLHTVCEEAHCPNIGECWNHRTATFLLLGDTCTRGCR
YCAIGKGKPKPIDEQEPQRIAAAVAHLKLKHVVLTSVNRDDQADGGAHIFAESIDLIRQQLPECKIEVLIPDFDGNWEAL
RIVMDARPDVLNHNIETVPRLFRKFRPRANFYQSLELLRLARDLQPSIVTKSGLMVGAGETDEEVLTVMDALRRSDVDVM
TIGQYLSPSGDHWPIDRYVPPETFTWFREQGLARGFRHVESGPMVRSSYHAHEHVAGL

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=47.3498233215548, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=47.5609756097561, Blast_Score=245, Evalue=4e-65,
Organism=Escherichia coli, GI1786846, Length=283, Percent_Identity=48.4098939929329, Blast_Score=285, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=42.6573426573427, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=295, Percent_Identity=44.0677966101695, Blast_Score=252, Evalue=5e-68,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.2897526501767, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33628; Mature: 33628

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTITLFEAPKAAPVAPRRPAWLKARAPIGENYEDVHGLMRELDLHTVCEEAHCPNIGEC
CCCEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
WNHRTATFLLLGDTCTRGCRYCAIGKGKPKPIDEQEPQRIAAAVAHLKLKHVVLTSVNRD
HCCCCEEEEEECCHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
DQADGGAHIFAESIDLIRQQLPECKIEVLIPDFDGNWEALRIVMDARPDVLNHNIETVPR
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCHHHCCCHHHHHH
LFRKFRPRANFYQSLELLRLARDLQPSIVTKSGLMVGAGETDEEVLTVMDALRRSDVDVM
HHHHHCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCEE
TIGQYLSPSGDHWPIDRYVPPETFTWFREQGLARGFRHVESGPMVRSSYHAHEHVAGL
EHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTITLFEAPKAAPVAPRRPAWLKARAPIGENYEDVHGLMRELDLHTVCEEAHCPNIGEC
CCCEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
WNHRTATFLLLGDTCTRGCRYCAIGKGKPKPIDEQEPQRIAAAVAHLKLKHVVLTSVNRD
HCCCCEEEEEECCHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
DQADGGAHIFAESIDLIRQQLPECKIEVLIPDFDGNWEALRIVMDARPDVLNHNIETVPR
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCHHHCCCHHHHHH
LFRKFRPRANFYQSLELLRLARDLQPSIVTKSGLMVGAGETDEEVLTVMDALRRSDVDVM
HHHHHCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCEE
TIGQYLSPSGDHWPIDRYVPPETFTWFREQGLARGFRHVESGPMVRSSYHAHEHVAGL
EHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA