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The map label for this gene is fadH [C]

Identifier: 159897115

GI number: 159897115

Start: 676455

End: 677354

Strand: Direct

Name: fadH [C]

Synonym: Haur_0586

Alternate gene names: 159897115

Gene position: 676455-677354 (Clockwise)

Preceding gene: 159897111

Following gene: 159897116

Centisome position: 10.66

GC content: 53.89

Gene sequence:

>900_bases
ATGCTACGTCTTTTTTCAGCGATCCAACTTGGTACACTCGAATTAACCAATCGCTTGGTCGCCACGCCTAGCCCAAGTCG
GTTTAGCCATGGCGGTGGTTTTCTCAATCGCAGCCTGATTGTCGAATACGGGCGTTGGAGCAGTTATGGCATTGGCTTGA
TCATCAGCGAGCCAGTCTTAGTGCAAGCTAACTCCAATCTGCCCCGCCCAGCCTTGAGCGCCGATGCTCATATTCAAGCA
TTTCGTAATTTGAACCAATGCTTGCACGAATATCAAAGCCATTGCTTGCCATTGTTGAGCTTGGTCAGCCAACCTGAATC
GCAACTGCAACCGAGCAACTGGCCTACTTTACGTGAACAGATGGTGTTGGCGGCTTGGCGAGCCTATGCTGCTGGCAGCC
CAGGGATTGTGATCGATGCCAATGCAGGCTCGCTTTTTCATCAGCTGCGGGTGAGCAAACAAGTGCCGCCAACCTCGCGC
AGCCAATTGTTGCTTGAAATCATCGAGGCAATTCGCGGCTGGCTTGGGCGGCAGTTTGTGGTTGGAGTGCGGTTGGTGGT
CGATGATTTAGTGCCCGATGGCGTGAGTTTGCAAGAAAGCCGCGTTTTGGCCCATGAATTAGTGGCCGCCGGAGTCGATT
TACTCGATTTGGTGGTTGGGCGCTCGGTCGCACCAATTGCCCAATTTCCTGGCTGGCAAATTCCCTTGGTGGCAGGCATT
CGCAGCATTGTGGATGTACCATTGATTGCCCATGGAATGTTGGGCGACCCGTGGATTGCCGAGGAAATGTTGCGCGAAGG
CTGTGCTGATTTGGTGGGGCTTGAAGCGGCAATTTCGGCTACACCGCATTGGCCAATTTGGGCACGCCAACAACTTGAGC
GCGAAGCCGAGGCGGCATGA

Upstream 100 bases:

>100_bases
ACAGTTCGGTGACACTCGGCCATTTAAGGCCATTTTTTAGGCCTTACCATCTTGTCAAAGCCTGTAGCCTCGGCGCACAA
TAGCAGCGGAGGTTTTGACA

Downstream 100 bases:

>100_bases
CGAGTGCCAGCCTGCGCCATGGTATAATAGCCAAACCATGGCGCTGAAAGAAGGATCGAAGATATGGCAAAAACAATCTT
CATCATGGATGATGATCTCG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLRLFSAIQLGTLELTNRLVATPSPSRFSHGGGFLNRSLIVEYGRWSSYGIGLIISEPVLVQANSNLPRPALSADAHIQA
FRNLNQCLHEYQSHCLPLLSLVSQPESQLQPSNWPTLREQMVLAAWRAYAAGSPGIVIDANAGSLFHQLRVSKQVPPTSR
SQLLLEIIEAIRGWLGRQFVVGVRLVVDDLVPDGVSLQESRVLAHELVAAGVDLLDLVVGRSVAPIAQFPGWQIPLVAGI
RSIVDVPLIAHGMLGDPWIAEEMLREGCADLVGLEAAISATPHWPIWARQQLEREAEAA

Sequences:

>Translated_299_residues
MLRLFSAIQLGTLELTNRLVATPSPSRFSHGGGFLNRSLIVEYGRWSSYGIGLIISEPVLVQANSNLPRPALSADAHIQA
FRNLNQCLHEYQSHCLPLLSLVSQPESQLQPSNWPTLREQMVLAAWRAYAAGSPGIVIDANAGSLFHQLRVSKQVPPTSR
SQLLLEIIEAIRGWLGRQFVVGVRLVVDDLVPDGVSLQESRVLAHELVAAGVDLLDLVVGRSVAPIAQFPGWQIPLVAGI
RSIVDVPLIAHGMLGDPWIAEEMLREGCADLVGLEAAISATPHWPIWARQQLEREAEAA
>Mature_299_residues
MLRLFSAIQLGTLELTNRLVATPSPSRFSHGGGFLNRSLIVEYGRWSSYGIGLIISEPVLVQANSNLPRPALSADAHIQA
FRNLNQCLHEYQSHCLPLLSLVSQPESQLQPSNWPTLREQMVLAAWRAYAAGSPGIVIDANAGSLFHQLRVSKQVPPTSR
SQLLLEIIEAIRGWLGRQFVVGVRLVVDDLVPDGVSLQESRVLAHELVAAGVDLLDLVVGRSVAPIAQFPGWQIPLVAGI
RSIVDVPLIAHGMLGDPWIAEEMLREGCADLVGLEAAISATPHWPIWARQQLEREAEAA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=310, Percent_Identity=24.1935483870968, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 32604; Mature: 32604

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLFSAIQLGTLELTNRLVATPSPSRFSHGGGFLNRSLIVEYGRWSSYGIGLIISEPVL
CCHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHCHHHEEEECCCCCCCCEEEECCCEE
VQANSNLPRPALSADAHIQAFRNLNQCLHEYQSHCLPLLSLVSQPESQLQPSNWPTLREQ
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHH
MVLAAWRAYAAGSPGIVIDANAGSLFHQLRVSKQVPPTSRSQLLLEIIEAIRGWLGRQFV
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHH
VGVRLVVDDLVPDGVSLQESRVLAHELVAAGVDLLDLVVGRSVAPIAQFPGWQIPLVAGI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHH
RSIVDVPLIAHGMLGDPWIAEEMLREGCADLVGLEAAISATPHWPIWARQQLEREAEAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRLFSAIQLGTLELTNRLVATPSPSRFSHGGGFLNRSLIVEYGRWSSYGIGLIISEPVL
CCHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHCHHHEEEECCCCCCCCEEEECCCEE
VQANSNLPRPALSADAHIQAFRNLNQCLHEYQSHCLPLLSLVSQPESQLQPSNWPTLREQ
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHH
MVLAAWRAYAAGSPGIVIDANAGSLFHQLRVSKQVPPTSRSQLLLEIIEAIRGWLGRQFV
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHH
VGVRLVVDDLVPDGVSLQESRVLAHELVAAGVDLLDLVVGRSVAPIAQFPGWQIPLVAGI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHH
RSIVDVPLIAHGMLGDPWIAEEMLREGCADLVGLEAAISATPHWPIWARQQLEREAEAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]