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The map label for this gene is cls [H]

Identifier: 159896920

GI number: 159896920

Start: 458411

End: 459631

Strand: Direct

Name: cls [H]

Synonym: Haur_0388

Alternate gene names: 159896920

Gene position: 458411-459631 (Clockwise)

Preceding gene: 159896919

Following gene: 159896921

Centisome position: 7.22

GC content: 49.3

Gene sequence:

>1221_bases
ATGCCGTTTGAGTCGTTTCAAGATCTGATAACAATGCTGGTGGTTATTCTCGTGCTCCAAGTGAGCTTGGCATTGTTGTT
AATTGGCGTGCGCAAATGGCGACCAACGCGCTATCAACGCTATCCGCGCATTAGCCTGCCACCAACCAGCGACGAGCGCC
ATCAATTTCAGTTGTATATGTCGGGCGAAGAGCTGTTTCCGGCCATGCTGCAAGCGATTGAATCGGCGCAGCGCTTGATT
TTGCTCGAAACCTTTATTTTCAAAGGCGATGATTTAGGCCATAAATTTCGTGAAGCCTTATTGCGCAAAGCTCGCAGCGG
CGTGGCCGTCTATGTGATTATCGATGGGTTTGCCAATTTGGTGGTCCCACGCAAATTCAAAAATTTTCCGCCGGAAATTC
AACTGTTGAGCTACCCAGCTTTTCCGCGTTTATCGCAGATTTTCGATATTGGTTCGTATGCGCGTGACCATCGCAAACTG
TTGGTCGTTGATAACGAAGTGGCCTTTATTGGGGGCTACAATTTGGGTGAATTGTATCGCACTGAGTGGCGCGATACTCA
TGTGCAGGTGCGTGGCCCGATTGTCAGCAATTTGGCCGAAATGTTTGCTTCGCAATGGAACCGTTATCGCCGCCGCCTAA
CGCGCTTGCACATCGACCTCGAGTTGGAATGGCAATCGACAGTGCGGGTACAGCGCAACGACGCTGGCCGCTTGGTTTTT
CCAATTCGTTCGATGTATCTTGATGCAATTGAACGAGCTGAACGGTTTGTTTACATTAGCAACGCCTATTTTATTCCCGA
TCGGGCGATTTTAGAGGCCTTGGTGCTGACCGCACGGCGCGGAGTTGATGTGCGCATTTTGGTTCCAGCTGAATCGAACC
ATGTGTTGGCCGATTGGCTGGCGCGGCATTATTTTGAATTTTGCCTGCGCCATGGGATTAAAATTTTTCTCTACGAAAAT
GCCATGATTCACGCCAAAACCGCTACGATCGATGGAATTTGGTCAACGGTTGGCACGGCCAATCTTGATCGGCTGAGTTT
AGCGGGCAACCATGAAATCAATCTAGAAATTTATAACGCCGCCTTTGCTGAACGGATGCAAGAAATTTTTGCCTGCGATA
TGGCTAATGCCCGTGAATTAACCTTGGCCGAATGGATTAAGCGCCCATGGTATACCAAGGCCGCCGAGTTGGTGCTCTCG
CCATTATGGCCAATTTTGTAG

Upstream 100 bases:

>100_bases
GTTTATTGATTGTGCCCGAAGCAGGTCATGGCATTAATCGTGGTGCAATGGCCGAAGTTGTGTTATTTGGGGAACTGCAC
TAGTTGAGGGAGCGTAGCGC

Downstream 100 bases:

>100_bases
AAATGGAGCAAGCATGCTCAAACGCAACGAATCTTTAGCCTTTGGCCGCCCATTTTTGATTACGGTGATTGCAATTGGCT
ATGTTTTGACGGCGATGTTG

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MPFESFQDLITMLVVILVLQVSLALLLIGVRKWRPTRYQRYPRISLPPTSDERHQFQLYMSGEELFPAMLQAIESAQRLI
LLETFIFKGDDLGHKFREALLRKARSGVAVYVIIDGFANLVVPRKFKNFPPEIQLLSYPAFPRLSQIFDIGSYARDHRKL
LVVDNEVAFIGGYNLGELYRTEWRDTHVQVRGPIVSNLAEMFASQWNRYRRRLTRLHIDLELEWQSTVRVQRNDAGRLVF
PIRSMYLDAIERAERFVYISNAYFIPDRAILEALVLTARRGVDVRILVPAESNHVLADWLARHYFEFCLRHGIKIFLYEN
AMIHAKTATIDGIWSTVGTANLDRLSLAGNHEINLEIYNAAFAERMQEIFACDMANARELTLAEWIKRPWYTKAAELVLS
PLWPIL

Sequences:

>Translated_406_residues
MPFESFQDLITMLVVILVLQVSLALLLIGVRKWRPTRYQRYPRISLPPTSDERHQFQLYMSGEELFPAMLQAIESAQRLI
LLETFIFKGDDLGHKFREALLRKARSGVAVYVIIDGFANLVVPRKFKNFPPEIQLLSYPAFPRLSQIFDIGSYARDHRKL
LVVDNEVAFIGGYNLGELYRTEWRDTHVQVRGPIVSNLAEMFASQWNRYRRRLTRLHIDLELEWQSTVRVQRNDAGRLVF
PIRSMYLDAIERAERFVYISNAYFIPDRAILEALVLTARRGVDVRILVPAESNHVLADWLARHYFEFCLRHGIKIFLYEN
AMIHAKTATIDGIWSTVGTANLDRLSLAGNHEINLEIYNAAFAERMQEIFACDMANARELTLAEWIKRPWYTKAAELVLS
PLWPIL
>Mature_405_residues
PFESFQDLITMLVVILVLQVSLALLLIGVRKWRPTRYQRYPRISLPPTSDERHQFQLYMSGEELFPAMLQAIESAQRLIL
LETFIFKGDDLGHKFREALLRKARSGVAVYVIIDGFANLVVPRKFKNFPPEIQLLSYPAFPRLSQIFDIGSYARDHRKLL
VVDNEVAFIGGYNLGELYRTEWRDTHVQVRGPIVSNLAEMFASQWNRYRRRLTRLHIDLELEWQSTVRVQRNDAGRLVFP
IRSMYLDAIERAERFVYISNAYFIPDRAILEALVLTARRGVDVRILVPAESNHVLADWLARHYFEFCLRHGIKIFLYENA
MIHAKTATIDGIWSTVGTANLDRLSLAGNHEINLEIYNAAFAERMQEIFACDMANARELTLAEWIKRPWYTKAAELVLSP
LWPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=357, Percent_Identity=32.2128851540616, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787502, Length=372, Percent_Identity=25.8064516129032, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47184; Mature: 47053

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFESFQDLITMLVVILVLQVSLALLLIGVRKWRPTRYQRYPRISLPPTSDERHQFQLYM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEEEE
SGEELFPAMLQAIESAQRLILLETFIFKGDDLGHKFREALLRKARSGVAVYVIIDGFANL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHH
VVPRKFKNFPPEIQLLSYPAFPRLSQIFDIGSYARDHRKLLVVDNEVAFIGGYNLGELYR
CCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCHHHHHH
TEWRDTHVQVRGPIVSNLAEMFASQWNRYRRRLTRLHIDLELEWQSTVRVQRNDAGRLVF
HCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEECCCCCEEH
PIRSMYLDAIERAERFVYISNAYFIPDRAILEALVLTARRGVDVRILVPAESNHVLADWL
HHHHHHHHHHHHCCEEEEEECEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
ARHYFEFCLRHGIKIFLYENAMIHAKTATIDGIWSTVGTANLDRLSLAGNHEINLEIYNA
HHHHHHHHHHCCCEEEEEECEEEEEEECCHHHHHHHHCCCCCCEEEECCCCEEEEEEEHH
AFAERMQEIFACDMANARELTLAEWIKRPWYTKAAELVLSPLWPIL
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PFESFQDLITMLVVILVLQVSLALLLIGVRKWRPTRYQRYPRISLPPTSDERHQFQLYM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEEEE
SGEELFPAMLQAIESAQRLILLETFIFKGDDLGHKFREALLRKARSGVAVYVIIDGFANL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHH
VVPRKFKNFPPEIQLLSYPAFPRLSQIFDIGSYARDHRKLLVVDNEVAFIGGYNLGELYR
CCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCHHHHHH
TEWRDTHVQVRGPIVSNLAEMFASQWNRYRRRLTRLHIDLELEWQSTVRVQRNDAGRLVF
HCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEECCCCCEEH
PIRSMYLDAIERAERFVYISNAYFIPDRAILEALVLTARRGVDVRILVPAESNHVLADWL
HHHHHHHHHHHHCCEEEEEECEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
ARHYFEFCLRHGIKIFLYENAMIHAKTATIDGIWSTVGTANLDRLSLAGNHEINLEIYNA
HHHHHHHHHHCCCEEEEEECEEEEEEECCHHHHHHHHCCCCCCEEEECCCCEEEEEEEHH
AFAERMQEIFACDMANARELTLAEWIKRPWYTKAAELVLSPLWPIL
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10585141 [H]