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The map label for this gene is cadA [H]

Identifier: 159046486

GI number: 159046486

Start: 15242

End: 17428

Strand: Direct

Name: cadA [H]

Synonym: Dshi_3947

Alternate gene names: 159046486

Gene position: 15242-17428 (Clockwise)

Preceding gene: 159046484

Following gene: 159046488

Centisome position: 12.07

GC content: 65.16

Gene sequence:

>2187_bases
GTGGCGATCCTGAACCGGGTTGTCGGGCCGAAAGTGGGCGGAACCGAACACCTGGCATTCGACGTGATCAACGGGCGAAT
GACCATCCTCGATAGCGCCGAGAGGATACCAGACGACGAAGTCACGCAGTTGGTCGCAACGACCGGCATGAGCGCCAAGC
CATGGGATGCCGACAACGCAGCAGAGGACCAGGCGGCGCATCTGGCACGTCAGAAGCGCTTTACCTCGTTGAGCGGCGGC
TTCTGGGCGGCGGGATTCCTTTTTCACATTGTCGAGACCGGCATGGGCGGCGCGCTCGGGCTCTTTGCGGGCCATGGCGA
GGTGCCTATGCCTATGGTGGAAGCAGGGATCTTCGCCGTCGCGATTCTTTTTGGCGTCTGGCTTGTCGCGCCCAAGGCCT
GGTCTTCGGCGCGGAGGCTTAGCCCCGACATGAACCTGCTCATGGTGGTGGCCGTCGCGGGCGCCATCGGCCTCGGCGAG
TTTTTTGAGGCCGCGACGGTCGCCTTCTTCTTCTCGCTTTCGCTTTATCTTGAGAGCTGGAGCGTGGGGCGGGCGCGTAA
TGCGGTTTCCGCGCTCCTCGATCTGGCGCCGCCAACGGCGCGGGTTCTTTACGACGACGGATCTGAATCCGACGTTCCGG
CCGCGGCCGTGGCAGTCAATGCAAGGTTTATCGTGCGCGGCGGCGACCGGATCCCCCTCGACGGTGAGGTCGTGGACGGG
GCAGGGGCCGTCGATCAGGCCCCAATTACTGGGGAAAGCGCGCTAGTGCCCAAGGAACCAGGCGACGAGGTCTATGCCGG
CACGATCAACGGCGAGGGCACACTGACGGTGCGGGCGACGAAGGCCGCCTCGGACACGGTGCTCGCGAAAATCATTCGCA
TGGTGGGCGATGCCCATGCCCGCCGCGCGCCGGTCGAACAGTGGGTGGCCAAGTTCGCGCGCATCTACACACCCATCGTG
ATGGCTTTGGCGATCGCCATCGCGCTGGTGCCGCCACTGCTGCTCGGCGGCGCCTGGGACTACTGGTTCTACAATGCTCT
GGTCCTTCTGGTCATCGCATGTCCTTGCGCGCTGGTCATTTCTACGCCGGTCTCCATCGTGGCAGCGCTGACCGCGTCGG
CACGGGCGGGGGTGCTCATCAAGGGCGGCGCCTATGTCGAGGCGCCGGGGCGGACCACGGCACTGGCGATGGACAAGACC
GGGACGATCACCATGGGCGAGCCTGAGGTGGCGGCGGTTCACCCACTGGTCGGCGTTTCCGCACGCGATCTCATGGCGCT
GGCAGCAAGCCTGGAGGCGCGGTCTTCACACCCGCTGGCACGCGCCATCCTCTCGCGCGCCGAAGCCGATGGTGTTTCCG
TGTCTGCCGCAGAGGACACCCGTACCGTGCCCGGGCGCGGTCTGGAAGGACGCGCCGACGGGCGCGCTATCTGGCTCGGC
TCGGATCGCTTCGCCGAAGAGAAGGGGTTCGGCAATGCCATTCCGGCGGATCTGCGGGATCGGATCGAAGGGGCAGGGAG
CACCCTCGTTGCCGTGGGTGACGAGACCGGCGTGACCGGCGTACTGGAGCTTCGCGACCGCATCCGCCCAGATGCAAAGG
GGATCGTTGCGCGTCTGCATGCGCAGGGTGTGAAGACCATCGTGATGTTGACGGGCGACAACGAACGCACCGCGCGCGCG
GTGGCAGCCGAAGTCGGCATCGACGAGGTGCGCGCCGAACTTTTGCCAGAAGACAAGGTGACGGCCATTGAGGAACTCGT
CGAAAGCCACGACATGGTGGCGATGATCGGCGACGGTGTGAATGACGCACCGGCCATGGCGCGGGCGCATTATGCCATCG
CCATGGGCGCTGTCGGATCGGATGCCGCGATCGAGACGGCCGACATCGCGCTGATGACCGACGACATCGGCAAGGTACCC
TGGCTTATCGGCCATTCGCGCCGTGCCATGACGATCATCCACCAGAATATCGGCATATCGCTGGCGACCAAGGCGCTGTT
CGTCGGGCTGACCGCGTTCGGTATGGCCTCAATGTGGGGCGCCATTGCTGCGGATGTAGGCGTGTCTCTGCTGGTGGTCG
CCAACGCACTTCGGCTCCTGAACGGCCATCAGGTTGAGGCCGGGCCTTCAGGCGGAGAACCGGTGGGCGAGCAGATGGCG
AAAGGAGCACTGGCGCACGGTCATTGA

Upstream 100 bases:

>100_bases
CAACTCCTGTTGCGACGCCAGCAAGGACGTCGCGTCCACGGCGCCAGCGCCGTCTGGCGGAAGGAGCTTTCAGGTCTCCG
GGCTCGATTGCGCCGAAGAG

Downstream 100 bases:

>100_bases
TCCGGCGGTTTCGGTAGCTCGATATCGCTTTTGCGGTCCAGCCACGCCAGCGCCCCGACAGAGAATTCCGAACCCCGGTA
GGGTTCCGTCCAGGCGGTCT

Product: heavy metal translocating P-type ATPase

Products: NA

Alternate protein names: Cadmium efflux ATPase [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MAILNRVVGPKVGGTEHLAFDVINGRMTILDSAERIPDDEVTQLVATTGMSAKPWDADNAAEDQAAHLARQKRFTSLSGG
FWAAGFLFHIVETGMGGALGLFAGHGEVPMPMVEAGIFAVAILFGVWLVAPKAWSSARRLSPDMNLLMVVAVAGAIGLGE
FFEAATVAFFFSLSLYLESWSVGRARNAVSALLDLAPPTARVLYDDGSESDVPAAAVAVNARFIVRGGDRIPLDGEVVDG
AGAVDQAPITGESALVPKEPGDEVYAGTINGEGTLTVRATKAASDTVLAKIIRMVGDAHARRAPVEQWVAKFARIYTPIV
MALAIAIALVPPLLLGGAWDYWFYNALVLLVIACPCALVISTPVSIVAALTASARAGVLIKGGAYVEAPGRTTALAMDKT
GTITMGEPEVAAVHPLVGVSARDLMALAASLEARSSHPLARAILSRAEADGVSVSAAEDTRTVPGRGLEGRADGRAIWLG
SDRFAEEKGFGNAIPADLRDRIEGAGSTLVAVGDETGVTGVLELRDRIRPDAKGIVARLHAQGVKTIVMLTGDNERTARA
VAAEVGIDEVRAELLPEDKVTAIEELVESHDMVAMIGDGVNDAPAMARAHYAIAMGAVGSDAAIETADIALMTDDIGKVP
WLIGHSRRAMTIIHQNIGISLATKALFVGLTAFGMASMWGAIAADVGVSLLVVANALRLLNGHQVEAGPSGGEPVGEQMA
KGALAHGH

Sequences:

>Translated_728_residues
MAILNRVVGPKVGGTEHLAFDVINGRMTILDSAERIPDDEVTQLVATTGMSAKPWDADNAAEDQAAHLARQKRFTSLSGG
FWAAGFLFHIVETGMGGALGLFAGHGEVPMPMVEAGIFAVAILFGVWLVAPKAWSSARRLSPDMNLLMVVAVAGAIGLGE
FFEAATVAFFFSLSLYLESWSVGRARNAVSALLDLAPPTARVLYDDGSESDVPAAAVAVNARFIVRGGDRIPLDGEVVDG
AGAVDQAPITGESALVPKEPGDEVYAGTINGEGTLTVRATKAASDTVLAKIIRMVGDAHARRAPVEQWVAKFARIYTPIV
MALAIAIALVPPLLLGGAWDYWFYNALVLLVIACPCALVISTPVSIVAALTASARAGVLIKGGAYVEAPGRTTALAMDKT
GTITMGEPEVAAVHPLVGVSARDLMALAASLEARSSHPLARAILSRAEADGVSVSAAEDTRTVPGRGLEGRADGRAIWLG
SDRFAEEKGFGNAIPADLRDRIEGAGSTLVAVGDETGVTGVLELRDRIRPDAKGIVARLHAQGVKTIVMLTGDNERTARA
VAAEVGIDEVRAELLPEDKVTAIEELVESHDMVAMIGDGVNDAPAMARAHYAIAMGAVGSDAAIETADIALMTDDIGKVP
WLIGHSRRAMTIIHQNIGISLATKALFVGLTAFGMASMWGAIAADVGVSLLVVANALRLLNGHQVEAGPSGGEPVGEQMA
KGALAHGH
>Mature_727_residues
AILNRVVGPKVGGTEHLAFDVINGRMTILDSAERIPDDEVTQLVATTGMSAKPWDADNAAEDQAAHLARQKRFTSLSGGF
WAAGFLFHIVETGMGGALGLFAGHGEVPMPMVEAGIFAVAILFGVWLVAPKAWSSARRLSPDMNLLMVVAVAGAIGLGEF
FEAATVAFFFSLSLYLESWSVGRARNAVSALLDLAPPTARVLYDDGSESDVPAAAVAVNARFIVRGGDRIPLDGEVVDGA
GAVDQAPITGESALVPKEPGDEVYAGTINGEGTLTVRATKAASDTVLAKIIRMVGDAHARRAPVEQWVAKFARIYTPIVM
ALAIAIALVPPLLLGGAWDYWFYNALVLLVIACPCALVISTPVSIVAALTASARAGVLIKGGAYVEAPGRTTALAMDKTG
TITMGEPEVAAVHPLVGVSARDLMALAASLEARSSHPLARAILSRAEADGVSVSAAEDTRTVPGRGLEGRADGRAIWLGS
DRFAEEKGFGNAIPADLRDRIEGAGSTLVAVGDETGVTGVLELRDRIRPDAKGIVARLHAQGVKTIVMLTGDNERTARAV
AAEVGIDEVRAELLPEDKVTAIEELVESHDMVAMIGDGVNDAPAMARAHYAIAMGAVGSDAAIETADIALMTDDIGKVPW
LIGHSRRAMTIIHQNIGISLATKALFVGLTAFGMASMWGAIAADVGVSLLVVANALRLLNGHQVEAGPSGGEPVGEQMAK
GALAHGH

Specific function: This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI115529486, Length=647, Percent_Identity=28.7480680061824, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI55743071, Length=625, Percent_Identity=30.72, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI55743073, Length=494, Percent_Identity=31.9838056680162, Blast_Score=216, Evalue=7e-56,
Organism=Homo sapiens, GI4502271, Length=259, Percent_Identity=25.4826254826255, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI83700225, Length=247, Percent_Identity=23.8866396761134, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI22748667, Length=259, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI48762687, Length=226, Percent_Identity=30.0884955752212, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI48762691, Length=226, Percent_Identity=30.0884955752212, Blast_Score=67, Evalue=7e-11,
Organism=Homo sapiens, GI48762685, Length=226, Percent_Identity=30.0884955752212, Blast_Score=67, Evalue=7e-11,
Organism=Homo sapiens, GI48762689, Length=226, Percent_Identity=30.0884955752212, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1789879, Length=588, Percent_Identity=40.9863945578231, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1786691, Length=556, Percent_Identity=38.4892086330935, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1786914, Length=445, Percent_Identity=34.8314606741573, Blast_Score=154, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17556548, Length=598, Percent_Identity=31.2709030100334, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17559224, Length=267, Percent_Identity=25.0936329588015, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320475, Length=492, Percent_Identity=30.0813008130081, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319772, Length=606, Percent_Identity=27.8877887788779, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321430, Length=558, Percent_Identity=25.6272401433692, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325221, Length=552, Percent_Identity=25.3623188405797, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321271, Length=245, Percent_Identity=28.9795918367347, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321432, Length=232, Percent_Identity=29.7413793103448, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221329854, Length=348, Percent_Identity=31.3218390804598, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24668708, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281366617, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=6e-11,
Organism=Drosophila melanogaster, GI161085803, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24668696, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24668704, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3 [H]

Molecular weight: Translated: 75661; Mature: 75530

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00154 ATPASE_E1_E2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNRVVGPKVGGTEHLAFDVINGRMTILDSAERIPDDEVTQLVATTGMSAKPWDADNA
CCHHHHHCCCCCCCCCEEEEEEECCEEEEECCHHHCCCHHHHHHHHHCCCCCCCCCCCCC
AEDQAAHLARQKRFTSLSGGFWAAGFLFHIVETGMGGALGLFAGHGEVPMPMVEAGIFAV
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHH
AILFGVWLVAPKAWSSARRLSPDMNLLMVVAVAGAIGLGEFFEAATVAFFFSLSLYLESW
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SVGRARNAVSALLDLAPPTARVLYDDGSESDVPAAAVAVNARFIVRGGDRIPLDGEVVDG
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEECC
AGAVDQAPITGESALVPKEPGDEVYAGTINGEGTLTVRATKAASDTVLAKIIRMVGDAHA
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHCCHHH
RRAPVEQWVAKFARIYTPIVMALAIAIALVPPLLLGGAWDYWFYNALVLLVIACPCALVI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
STPVSIVAALTASARAGVLIKGGAYVEAPGRTTALAMDKTGTITMGEPEVAAVHPLVGVS
HHHHHHHHHHHHCCCCCEEEECCEEEECCCCEEEEEECCCCEEEECCCCCEEECCHHCCC
ARDLMALAASLEARSSHPLARAILSRAEADGVSVSAAEDTRTVPGRGLEGRADGRAIWLG
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEC
SDRFAEEKGFGNAIPADLRDRIEGAGSTLVAVGDETGVTGVLELRDRIRPDAKGIVARLH
CCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHH
AQGVKTIVMLTGDNERTARAVAAEVGIDEVRAELLPEDKVTAIEELVESHDMVAMIGDGV
HCCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCC
NDAPAMARAHYAIAMGAVGSDAAIETADIALMTDDIGKVPWLIGHSRRAMTIIHQNIGIS
CCCHHHHHHHHHEEECCCCCCCCEEEEEEEEEECCCCCCCEEECCCCCEEEEEECCCCHH
LATKALFVGLTAFGMASMWGAIAADVGVSLLVVANALRLLNGHQVEAGPSGGEPVGEQMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHH
KGALAHGH
CCCCCCCC
>Mature Secondary Structure 
AILNRVVGPKVGGTEHLAFDVINGRMTILDSAERIPDDEVTQLVATTGMSAKPWDADNA
CHHHHHCCCCCCCCCEEEEEEECCEEEEECCHHHCCCHHHHHHHHHCCCCCCCCCCCCC
AEDQAAHLARQKRFTSLSGGFWAAGFLFHIVETGMGGALGLFAGHGEVPMPMVEAGIFAV
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHH
AILFGVWLVAPKAWSSARRLSPDMNLLMVVAVAGAIGLGEFFEAATVAFFFSLSLYLESW
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SVGRARNAVSALLDLAPPTARVLYDDGSESDVPAAAVAVNARFIVRGGDRIPLDGEVVDG
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEECC
AGAVDQAPITGESALVPKEPGDEVYAGTINGEGTLTVRATKAASDTVLAKIIRMVGDAHA
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHCCHHH
RRAPVEQWVAKFARIYTPIVMALAIAIALVPPLLLGGAWDYWFYNALVLLVIACPCALVI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
STPVSIVAALTASARAGVLIKGGAYVEAPGRTTALAMDKTGTITMGEPEVAAVHPLVGVS
HHHHHHHHHHHHCCCCCEEEECCEEEECCCCEEEEEECCCCEEEECCCCCEEECCHHCCC
ARDLMALAASLEARSSHPLARAILSRAEADGVSVSAAEDTRTVPGRGLEGRADGRAIWLG
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEC
SDRFAEEKGFGNAIPADLRDRIEGAGSTLVAVGDETGVTGVLELRDRIRPDAKGIVARLH
CCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHH
AQGVKTIVMLTGDNERTARAVAAEVGIDEVRAELLPEDKVTAIEELVESHDMVAMIGDGV
HCCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCC
NDAPAMARAHYAIAMGAVGSDAAIETADIALMTDDIGKVPWLIGHSRRAMTIIHQNIGIS
CCCHHHHHHHHHEEECCCCCCCCEEEEEEEEEECCCCCCCEEECCCCCEEEEEECCCCHH
LATKALFVGLTAFGMASMWGAIAADVGVSLLVVANALRLLNGHQVEAGPSGGEPVGEQMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHH
KGALAHGH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11302791 [H]