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The map label for this gene is rhtB [H]

Identifier: 158423951

GI number: 158423951

Start: 2649627

End: 2650238

Strand: Reverse

Name: rhtB [H]

Synonym: AZC_2327

Alternate gene names: 158423951

Gene position: 2650238-2649627 (Counterclockwise)

Preceding gene: 158423952

Following gene: 158423950

Centisome position: 49.35

GC content: 69.93

Gene sequence:

>612_bases
ATGATCTCCGCAGAGAGCCTGGCCATCTTCATCCTCGCGTGCCTCGCTCTCTGCGCGACGCCCGGCCCCAATGTGGCGCT
CATTGTCGGCACCAGCCTGAAGCACGGGCGCAAGGCCGGCATGCTGAGCGCGCTCGGGGTCAATGTCGGCATCATCCTCC
AGCTCGCCGCCGTGGCGGCGGGCCTTTCCTGGCTGGTGGACCTGTTCGCCCGCCATTTCGACGTGATCCGCTATCTCGGC
GCGGCCTATCTGATCTGGCTGGCCGTGTTGCAATGGCGCGGTGCCGGCAAGATGGGCGACAAACCCATGCCCACCCGCCG
GGCGTCCTTCGGGCGTGGCTTCGCGGTCGCCTTCGCCAATCCCAAGACGCTGCTGTTCCACGCGGCCTTCCTGCCGCAGT
TCGTCACCAACACCGCCGATCCCGGCCCGCAGATCGCGCTGCTTGCGGCCATCTTCGCGCTGATCGCGTTGGTGGGCGAC
ATGATCTGGGCAGTGGTGGCGGACCGGGCCCGCGCGGCGCTCGCCGGCCGCTTCACCCGCATCGCCGACCGGGTGTCCGC
CGGCATTCTCGCCGGCGGGGCCTTGGTGCTGCTCGCCGCCGGCCGGCGCTGA

Upstream 100 bases:

>100_bases
GGTCTCGGTGGAGCACATCAGCGAGGACGAGGCGGGCGAAGCCACCGAGGGCGAAGGCGAGGAGACCGGCGCCCCCGACG
AGAGCCCCGAGGCCTGATCC

Downstream 100 bases:

>100_bases
CCCGCCCCATTGCGCTGCGCGCCGTCTAAGGCTACCCCATCGCCATGAGCCGACCCGACCACACGCGCCGCGTTGCCATC
TATGCCGGTTCGTTCGATCC

Product: homoserine/homoserine lactone/threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MISAESLAIFILACLALCATPGPNVALIVGTSLKHGRKAGMLSALGVNVGIILQLAAVAAGLSWLVDLFARHFDVIRYLG
AAYLIWLAVLQWRGAGKMGDKPMPTRRASFGRGFAVAFANPKTLLFHAAFLPQFVTNTADPGPQIALLAAIFALIALVGD
MIWAVVADRARAALAGRFTRIADRVSAGILAGGALVLLAAGRR

Sequences:

>Translated_203_residues
MISAESLAIFILACLALCATPGPNVALIVGTSLKHGRKAGMLSALGVNVGIILQLAAVAAGLSWLVDLFARHFDVIRYLG
AAYLIWLAVLQWRGAGKMGDKPMPTRRASFGRGFAVAFANPKTLLFHAAFLPQFVTNTADPGPQIALLAAIFALIALVGD
MIWAVVADRARAALAGRFTRIADRVSAGILAGGALVLLAAGRR
>Mature_203_residues
MISAESLAIFILACLALCATPGPNVALIVGTSLKHGRKAGMLSALGVNVGIILQLAAVAAGLSWLVDLFARHFDVIRYLG
AAYLIWLAVLQWRGAGKMGDKPMPTRRASFGRGFAVAFANPKTLLFHAAFLPQFVTNTADPGPQIALLAAIFALIALVGD
MIWAVVADRARAALAGRFTRIADRVSAGILAGGALVLLAAGRR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=166, Percent_Identity=31.9277108433735, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788099, Length=155, Percent_Identity=29.6774193548387, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786522, Length=195, Percent_Identity=31.2820512820513, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21216; Mature: 21216

Theoretical pI: Translated: 11.64; Mature: 11.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAESLAIFILACLALCATPGPNVALIVGTSLKHGRKAGMLSALGVNVGIILQLAAVAA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
GLSWLVDLFARHFDVIRYLGAAYLIWLAVLQWRGAGKMGDKPMPTRRASFGRGFAVAFAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEECC
PKTLLFHAAFLPQFVTNTADPGPQIALLAAIFALIALVGDMIWAVVADRARAALAGRFTR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADRVSAGILAGGALVLLAAGRR
HHHHHHHHHHHCCHHEEEECCCC
>Mature Secondary Structure
MISAESLAIFILACLALCATPGPNVALIVGTSLKHGRKAGMLSALGVNVGIILQLAAVAA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
GLSWLVDLFARHFDVIRYLGAAYLIWLAVLQWRGAGKMGDKPMPTRRASFGRGFAVAFAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEECC
PKTLLFHAAFLPQFVTNTADPGPQIALLAAIFALIALVGDMIWAVVADRARAALAGRFTR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADRVSAGILAGGALVLLAAGRR
HHHHHHHHHHHCCHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]