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The map label for this gene is hipO [H]

Identifier: 158423821

GI number: 158423821

Start: 2510362

End: 2511528

Strand: Direct

Name: hipO [H]

Synonym: AZC_2197

Alternate gene names: 158423821

Gene position: 2510362-2511528 (Clockwise)

Preceding gene: 158423819

Following gene: 158423824

Centisome position: 46.75

GC content: 69.07

Gene sequence:

>1167_bases
ATGCCCGTTCTGAATCGCGTTGCCGAAACCGCCGCCGAAATCGCCGCGTGGCGCCGTGAACTGCACGAATACCCCGAATT
GATGTTCGATCTGCCCCGCACCTCCGCCTTCGTGGCGGAGAAGCTGAGGGGGTTCGGCTGCGACGAGGTGGTGACGGGCA
TCGGGCGCAGCGGCGTGGTGGCGGTCATCCACGGCCGCGAGCGCGGAGCGGGGCGCGTCATCGGATTGCGCGCCGACATG
GACGCGCTTCCGGTGGAAGAAGAGACCGGCGCGGCCCATGCCTCGAAGGTGCCGGGCAAGATGCACGCCTGCGGCCATGA
CGGCCACACCGCCATGCTGCTGGGCGCGGCCCGGCATCTTGCCGAGACGCGGGCCTTCGCGGGCACGGCGGTGCTCATCT
TCCAGCCCGCCGAGGAAGGCGAGGGCGGCGCCCGCGTGATGATCGAGGACGGACTGCTGGAGTGCTTCCGCCCCGAGGAA
ATCTACGGCATGCACAATATGCCGGGCATTCCGGTCGGCCATTTCGCCATGCGGCCGGGCGCCATCATGGCCTCCACCGA
CCGGCTCGAGATCACGGTGGACGGTACCGGTGCCCATGCGGCGGCACCTCACCGGGGCGTCGATCCGGTGCTGGTGGGCT
CGGCCATCGTCATGGGGCTCCAGCAGGCGGTCGCGCGCAATGTGGACCCGCTCGAGGCGGCGGTCGTGTCCATCACCATG
TTCCATGCCGGCGCGGTGGAGAACGTGATTCCGCCCAAGGCGGAACTCACGGGCACCGTGCGCACCCTGAAGGCGCAGGT
ACGCGATCAACTGCGCCAGCGGCTGCGGGAGATCGTCTCCAAGATCGCGGAGGCCTACGGCGCCCGTGCGGAGCTGCGCT
GGATCGACGGCTATCCGCCGACCGTCAACCATCCCGGGCAGGCCGATTTCGCCGCGCGGGTGGCCCGCGACGTAGCCGGG
GCGGACAAGGTGGATGCGGACACGACGCCCATCATGGCGGCCGAGGACTTCTCCTTCATGCTGGAGGCCCGGCCGGGGGC
GTTCATCTTCGTGGGCAACGGCGACAGCGCGGGCCTGCACAACCCGCGCTATGATTTCGATGATGCCGCCATTCCCTACG
GTACGTCCTTCTGGGTGCGCCTCGTGGAGAGCGCCCTGCCGCTGTAA

Upstream 100 bases:

>100_bases
AGGGAGGGCAAGCCGTTCCCGCTGCGCTTCCGCATCGCAGCATCGGCGCTTGACCCCATACCGGCCCGGTTCCACGATCC
GGCTTCGACAGGAGCCCAAG

Downstream 100 bases:

>100_bases
GCGGGCGCTTCAGGCGCCCGTCTTACGGGCCGGTCTTGGGCGCAGAAGTCTTGGGCGCGCAGGTCTTGCGCAGGCGGAAG
GTGATGACGCCGTCCGTCAC

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPVLNRVAETAAEIAAWRRELHEYPELMFDLPRTSAFVAEKLRGFGCDEVVTGIGRSGVVAVIHGRERGAGRVIGLRADM
DALPVEEETGAAHASKVPGKMHACGHDGHTAMLLGAARHLAETRAFAGTAVLIFQPAEEGEGGARVMIEDGLLECFRPEE
IYGMHNMPGIPVGHFAMRPGAIMASTDRLEITVDGTGAHAAAPHRGVDPVLVGSAIVMGLQQAVARNVDPLEAAVVSITM
FHAGAVENVIPPKAELTGTVRTLKAQVRDQLRQRLREIVSKIAEAYGARAELRWIDGYPPTVNHPGQADFAARVARDVAG
ADKVDADTTPIMAAEDFSFMLEARPGAFIFVGNGDSAGLHNPRYDFDDAAIPYGTSFWVRLVESALPL

Sequences:

>Translated_388_residues
MPVLNRVAETAAEIAAWRRELHEYPELMFDLPRTSAFVAEKLRGFGCDEVVTGIGRSGVVAVIHGRERGAGRVIGLRADM
DALPVEEETGAAHASKVPGKMHACGHDGHTAMLLGAARHLAETRAFAGTAVLIFQPAEEGEGGARVMIEDGLLECFRPEE
IYGMHNMPGIPVGHFAMRPGAIMASTDRLEITVDGTGAHAAAPHRGVDPVLVGSAIVMGLQQAVARNVDPLEAAVVSITM
FHAGAVENVIPPKAELTGTVRTLKAQVRDQLRQRLREIVSKIAEAYGARAELRWIDGYPPTVNHPGQADFAARVARDVAG
ADKVDADTTPIMAAEDFSFMLEARPGAFIFVGNGDSAGLHNPRYDFDDAAIPYGTSFWVRLVESALPL
>Mature_387_residues
PVLNRVAETAAEIAAWRRELHEYPELMFDLPRTSAFVAEKLRGFGCDEVVTGIGRSGVVAVIHGRERGAGRVIGLRADMD
ALPVEEETGAAHASKVPGKMHACGHDGHTAMLLGAARHLAETRAFAGTAVLIFQPAEEGEGGARVMIEDGLLECFRPEEI
YGMHNMPGIPVGHFAMRPGAIMASTDRLEITVDGTGAHAAAPHRGVDPVLVGSAIVMGLQQAVARNVDPLEAAVVSITMF
HAGAVENVIPPKAELTGTVRTLKAQVRDQLRQRLREIVSKIAEAYGARAELRWIDGYPPTVNHPGQADFAARVARDVAGA
DKVDADTTPIMAAEDFSFMLEARPGAFIFVGNGDSAGLHNPRYDFDDAAIPYGTSFWVRLVESALPL

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=421, Percent_Identity=28.0285035629454, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41490; Mature: 41359

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLNRVAETAAEIAAWRRELHEYPELMFDLPRTSAFVAEKLRGFGCDEVVTGIGRSGVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCCCCEE
AVIHGRERGAGRVIGLRADMDALPVEEETGAAHASKVPGKMHACGHDGHTAMLLGAARHL
EEEECCCCCCCCEEEEECCCCCCCCCHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
AETRAFAGTAVLIFQPAEEGEGGARVMIEDGLLECFRPEEIYGMHNMPGIPVGHFAMRPG
HHHHHHCCEEEEEEECCCCCCCCCEEEEECCHHHHCCCHHHCCCCCCCCCCCCHHHCCCC
AIMASTDRLEITVDGTGAHAAAPHRGVDPVLVGSAIVMGLQQAVARNVDPLEAAVVSITM
CEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FHAGAVENVIPPKAELTGTVRTLKAQVRDQLRQRLREIVSKIAEAYGARAELRWIDGYPP
HHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
TVNHPGQADFAARVARDVAGADKVDADTTPIMAAEDFSFMLEARPGAFIFVGNGDSAGLH
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCCC
NPRYDFDDAAIPYGTSFWVRLVESALPL
CCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVLNRVAETAAEIAAWRRELHEYPELMFDLPRTSAFVAEKLRGFGCDEVVTGIGRSGVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCCCCEE
AVIHGRERGAGRVIGLRADMDALPVEEETGAAHASKVPGKMHACGHDGHTAMLLGAARHL
EEEECCCCCCCCEEEEECCCCCCCCCHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
AETRAFAGTAVLIFQPAEEGEGGARVMIEDGLLECFRPEEIYGMHNMPGIPVGHFAMRPG
HHHHHHCCEEEEEEECCCCCCCCCEEEEECCHHHHCCCHHHCCCCCCCCCCCCHHHCCCC
AIMASTDRLEITVDGTGAHAAAPHRGVDPVLVGSAIVMGLQQAVARNVDPLEAAVVSITM
CEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FHAGAVENVIPPKAELTGTVRTLKAQVRDQLRQRLREIVSKIAEAYGARAELRWIDGYPP
HHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
TVNHPGQADFAARVARDVAGADKVDADTTPIMAAEDFSFMLEARPGAFIFVGNGDSAGLH
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCCC
NPRYDFDDAAIPYGTSFWVRLVESALPL
CCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]