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The map label for this gene is leuD [H]

Identifier: 158423731

GI number: 158423731

Start: 2424342

End: 2424956

Strand: Direct

Name: leuD [H]

Synonym: AZC_2107

Alternate gene names: 158423731

Gene position: 2424342-2424956 (Clockwise)

Preceding gene: 158423730

Following gene: 158423732

Centisome position: 45.15

GC content: 66.5

Gene sequence:

>615_bases
ATGCAGCCCTTCATCCGGCTCGAATCCATCGGCTGCCCGCTCCCGCTCACGGGCGTTGACACCGACCAGCTTATCCCCGC
GCGCTTCATGAAGCGCTCGCGGGCGGAAGGCTATGGCGGCTATCTGCTGCATGACCTGCGGTTTGAGGGGGAGGGCAAGC
CGATTGCCGACTTCCCGCTCAACCAGCCGCTCTATCAGAGCGCGGAAGTGCTGGTGGCGCGGCGCAATTTCGGCAGCGGC
TCGTCCCGTGAGGCGGCGGTCTATGCGCTGGCGGACTTCGGCTTCCGTTGCGTTGTGGCACCCAGTTTCGGCGACATCTT
CGCCTCCAACGCCGTGAACAACGGCCTGCTGCCCGCACGCGTCTCGGAGGCTGATTGCGAGGCGCTGCTGGCGGGGCTCG
CAACGCAGAATCGCATGGTGGTGGACCTTGAGGCCTGCACCATCGTCTGCGGCAATCTGAGCGTCCCCTTCGAGGTGGAC
CCCGTGTGGCGCACCAAGCTGCTCAATGGTTGGGACGACATCGACCTCACCCTGAGCCGGCAGGCGGAAATCGCCGCCTT
CACGGCCTCCGACGCGAGCCTGCGCCCATGGATGGCGCCGCGCCGTCCGGAATGA

Upstream 100 bases:

>100_bases
CCGGCGCCCGCACGCACCTCATGTCTCCCGCCATGGTGGCTGCGGCGGCCGTGACGGGCCATCTCACCGACATGCGCCCG
CTGCTTGAGGGGAGGAACTG

Downstream 100 bases:

>100_bases
TGCGCAAAGCTCCACCTGGGCGCTTGACAGCAGCCGGGTGGAGCGGTCTCTTAAGACAGAAACCTGTCTTATATTAAAGA
CAGATCAGGGATGCCCATGT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MQPFIRLESIGCPLPLTGVDTDQLIPARFMKRSRAEGYGGYLLHDLRFEGEGKPIADFPLNQPLYQSAEVLVARRNFGSG
SSREAAVYALADFGFRCVVAPSFGDIFASNAVNNGLLPARVSEADCEALLAGLATQNRMVVDLEACTIVCGNLSVPFEVD
PVWRTKLLNGWDDIDLTLSRQAEIAAFTASDASLRPWMAPRRPE

Sequences:

>Translated_204_residues
MQPFIRLESIGCPLPLTGVDTDQLIPARFMKRSRAEGYGGYLLHDLRFEGEGKPIADFPLNQPLYQSAEVLVARRNFGSG
SSREAAVYALADFGFRCVVAPSFGDIFASNAVNNGLLPARVSEADCEALLAGLATQNRMVVDLEACTIVCGNLSVPFEVD
PVWRTKLLNGWDDIDLTLSRQAEIAAFTASDASLRPWMAPRRPE
>Mature_204_residues
MQPFIRLESIGCPLPLTGVDTDQLIPARFMKRSRAEGYGGYLLHDLRFEGEGKPIADFPLNQPLYQSAEVLVARRNFGSG
SSREAAVYALADFGFRCVVAPSFGDIFASNAVNNGLLPARVSEADCEALLAGLATQNRMVVDLEACTIVCGNLSVPFEVD
PVWRTKLLNGWDDIDLTLSRQAEIAAFTASDASLRPWMAPRRPE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=43.298969072165, Blast_Score=153, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=40.6091370558376, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22294; Mature: 22294

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFIRLESIGCPLPLTGVDTDQLIPARFMKRSRAEGYGGYLLHDLRFEGEGKPIADFPL
CCCCEEECCCCCCCCCCCCCCHHCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCCC
NQPLYQSAEVLVARRNFGSGSSREAAVYALADFGFRCVVAPSFGDIFASNAVNNGLLPAR
CCCHHHHHEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCC
VSEADCEALLAGLATQNRMVVDLEACTIVCGNLSVPFEVDPVWRTKLLNGWDDIDLTLSR
CCHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCEEECCHHHHHHCCCCCCEEEEECC
QAEIAAFTASDASLRPWMAPRRPE
CCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPFIRLESIGCPLPLTGVDTDQLIPARFMKRSRAEGYGGYLLHDLRFEGEGKPIADFPL
CCCCEEECCCCCCCCCCCCCCHHCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCCC
NQPLYQSAEVLVARRNFGSGSSREAAVYALADFGFRCVVAPSFGDIFASNAVNNGLLPAR
CCCHHHHHEEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCC
VSEADCEALLAGLATQNRMVVDLEACTIVCGNLSVPFEVDPVWRTKLLNGWDDIDLTLSR
CCHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCEEECCHHHHHHCCCCCCEEEEECC
QAEIAAFTASDASLRPWMAPRRPE
CCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA