Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 158423730

GI number: 158423730

Start: 2422927

End: 2424342

Strand: Direct

Name: leuC [H]

Synonym: AZC_2106

Alternate gene names: 158423730

Gene position: 2422927-2424342 (Clockwise)

Preceding gene: 158423728

Following gene: 158423731

Centisome position: 45.12

GC content: 68.43

Gene sequence:

>1416_bases
ATGGCGGCGAAGACCGCGCGAACCCTGTTCGACAAGGTGTGGGACGCGCATGTGGTGGTGCAGCGGGAGGACGGCCAGAG
CCTGCTCTGGATCGACCGTCACTTCCTGCACGAGGGTTCTTTCCATGCCTTCGACAAGGTGGCTGGCCGTGGCGCTTCGC
TGGCGCGGCCGGACCTCACCTTTGGCGTCGAGGACCATTATGTGCCCACCCGCGTGCGGCAGCTGGACCTGATCGATCCC
GCCATCCGCCGCATGATGGAACAGCTGCGCGCCAACACGCTGCGCCATAAGGTGCGCCTGTTCGATGTGGATGATCCTGC
GCAGGGCATCGTCCATGTGGTGGGGCCGGAGCAGGGGCTGACGCTGCCTGGCCTTTCCATCGTCTGCGGCGACAGTCACA
CGGCAACCCATGGCGCGTTCGGCGCGCTTGCCTTCGGCATCGGTGCCTCCGAGGTTGCCCATGTGCTGATGACGCAGACG
CTGTGGCAGAACAGGCCCAAGCGCTTGCGCATCCGCGTGGAGGGCAGGCTGGCGCCGGGCATCGTCGCCAAGGACGTGGC
GCTCTCCATCATCGCCGCCATCGGCGCGGATGGCGCGGCTGGCCATGCCATCGAATATGCAGGCTCGGTCGTGCGCGGCC
TCTCCATGGAAGGGCGGCTCACGCTCTGCAACCTCTCCATCGAGGCGGGCGGGCGCTGCGGCATGATCGCCCCTGACGCC
ACGACCCTCGATTATGTGCGCGGGCGGCCCTATGCGCCCGAAGGCGCGTTGTTCGATGCGGCGGCGGAGGCGTGGCTGGC
GCTCGGCAGTGATGCGGATGCGGAATTTGACCGTGAGGTGGTGCTGGATGGCGATGCCATAGCGCCCATCGTCACTTGGG
GCACGAGCCCCGAGGATGCGCTGCCCATCACCGCCACCGTGCCTGATCCCGAGCGGGTGGACGACGCGGCGAAGGCCGCC
CACATCCGCGATGCCGTCTCCTACATGGACCTGCGGCCGGGCCAGCGGCTCAGCGACATTGCGGTGGACCGCGTCTTCAT
CGGCTCCTGCACCAACAGCCGGATCGAGGATCTGCGCGCCGCCGCCGCCGTTCTAGCGGGGCGTTCCGCCAAGGTGCCGG
GGCTCGTGTCGCCCGGTTCCGCACTGGTGAAGCGGCAGGCGGAGGAGGAGGGACTGGACGCCATCTTCCGCGCCGCCGGC
CTTGAATGGGTGGAAGCCGGCTGCTCCATGTGCGTCGGCATGAATGGCGATCTCGTGCCGGCCGGTGAGCGCTGCGCCTC
CACCACCAATCGCAATTTCAAGGGCCGGCAGGGGCCCGGCGCCCGCACGCACCTCATGTCTCCCGCCATGGTGGCTGCGG
CGGCCGTGACGGGCCATCTCACCGACATGCGCCCGCTGCTTGAGGGGAGGAACTGA

Upstream 100 bases:

>100_bases
CTCGGTGCAGGACAGTAAGCTGTACTAGATATAAAACAAGTATTGCCTCTCGCGCCGGAACGGGCCTACCCTTTGGCACA
GCATTTCCGGAGGCGACGCA

Downstream 100 bases:

>100_bases
TGCAGCCCTTCATCCGGCTCGAATCCATCGGCTGCCCGCTCCCGCTCACGGGCGTTGACACCGACCAGCTTATCCCCGCG
CGCTTCATGAAGCGCTCGCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAAKTARTLFDKVWDAHVVVQREDGQSLLWIDRHFLHEGSFHAFDKVAGRGASLARPDLTFGVEDHYVPTRVRQLDLIDP
AIRRMMEQLRANTLRHKVRLFDVDDPAQGIVHVVGPEQGLTLPGLSIVCGDSHTATHGAFGALAFGIGASEVAHVLMTQT
LWQNRPKRLRIRVEGRLAPGIVAKDVALSIIAAIGADGAAGHAIEYAGSVVRGLSMEGRLTLCNLSIEAGGRCGMIAPDA
TTLDYVRGRPYAPEGALFDAAAEAWLALGSDADAEFDREVVLDGDAIAPIVTWGTSPEDALPITATVPDPERVDDAAKAA
HIRDAVSYMDLRPGQRLSDIAVDRVFIGSCTNSRIEDLRAAAAVLAGRSAKVPGLVSPGSALVKRQAEEEGLDAIFRAAG
LEWVEAGCSMCVGMNGDLVPAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDMRPLLEGRN

Sequences:

>Translated_471_residues
MAAKTARTLFDKVWDAHVVVQREDGQSLLWIDRHFLHEGSFHAFDKVAGRGASLARPDLTFGVEDHYVPTRVRQLDLIDP
AIRRMMEQLRANTLRHKVRLFDVDDPAQGIVHVVGPEQGLTLPGLSIVCGDSHTATHGAFGALAFGIGASEVAHVLMTQT
LWQNRPKRLRIRVEGRLAPGIVAKDVALSIIAAIGADGAAGHAIEYAGSVVRGLSMEGRLTLCNLSIEAGGRCGMIAPDA
TTLDYVRGRPYAPEGALFDAAAEAWLALGSDADAEFDREVVLDGDAIAPIVTWGTSPEDALPITATVPDPERVDDAAKAA
HIRDAVSYMDLRPGQRLSDIAVDRVFIGSCTNSRIEDLRAAAAVLAGRSAKVPGLVSPGSALVKRQAEEEGLDAIFRAAG
LEWVEAGCSMCVGMNGDLVPAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDMRPLLEGRN
>Mature_470_residues
AAKTARTLFDKVWDAHVVVQREDGQSLLWIDRHFLHEGSFHAFDKVAGRGASLARPDLTFGVEDHYVPTRVRQLDLIDPA
IRRMMEQLRANTLRHKVRLFDVDDPAQGIVHVVGPEQGLTLPGLSIVCGDSHTATHGAFGALAFGIGASEVAHVLMTQTL
WQNRPKRLRIRVEGRLAPGIVAKDVALSIIAAIGADGAAGHAIEYAGSVVRGLSMEGRLTLCNLSIEAGGRCGMIAPDAT
TLDYVRGRPYAPEGALFDAAAEAWLALGSDADAEFDREVVLDGDAIAPIVTWGTSPEDALPITATVPDPERVDDAAKAAH
IRDAVSYMDLRPGQRLSDIAVDRVFIGSCTNSRIEDLRAAAAVLAGRSAKVPGLVSPGSALVKRQAEEEGLDAIFRAAGL
EWVEAGCSMCVGMNGDLVPAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDMRPLLEGRN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=406, Percent_Identity=24.8768472906404, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI41352693, Length=420, Percent_Identity=26.1904761904762, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4501867, Length=443, Percent_Identity=25.2821670428894, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=53.8793103448276, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787531, Length=404, Percent_Identity=26.980198019802, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081781, Length=363, Percent_Identity=25.3443526170799, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=376, Percent_Identity=29.5212765957447, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564738, Length=376, Percent_Identity=29.5212765957447, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=420, Percent_Identity=24.7619047619048, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25149342, Length=326, Percent_Identity=28.5276073619632, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=53.3755274261603, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320440, Length=420, Percent_Identity=25.2380952380952, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=384, Percent_Identity=27.8645833333333, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323335, Length=368, Percent_Identity=27.445652173913, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=27.2321428571429, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=27.2321428571429, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161076999, Length=431, Percent_Identity=26.9141531322506, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571643, Length=450, Percent_Identity=26.2222222222222, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645686, Length=402, Percent_Identity=26.1194029850746, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17137564, Length=433, Percent_Identity=25.8660508083141, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50129; Mature: 49997

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTARTLFDKVWDAHVVVQREDGQSLLWIDRHFLHEGSFHAFDKVAGRGASLARPDLT
CCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEHHHHCCCCCHHHHHHHCCCCCCCCCCCE
FGVEDHYVPTRVRQLDLIDPAIRRMMEQLRANTLRHKVRLFDVDDPAQGIVHVVGPEQGL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCC
TLPGLSIVCGDSHTATHGAFGALAFGIGASEVAHVLMTQTLWQNRPKRLRIRVEGRLAPG
CCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
IVAKDVALSIIAAIGADGAAGHAIEYAGSVVRGLSMEGRLTLCNLSIEAGGRCGMIAPDA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCC
TTLDYVRGRPYAPEGALFDAAAEAWLALGSDADAEFDREVVLDGDAIAPIVTWGTSPEDA
CHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCC
LPITATVPDPERVDDAAKAAHIRDAVSYMDLRPGQRLSDIAVDRVFIGSCTNSRIEDLRA
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCCHHHHHHHH
AAAVLAGRSAKVPGLVSPGSALVKRQAEEEGLDAIFRAAGLEWVEAGCSMCVGMNGDLVP
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHEEECCCCCCCC
AGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDMRPLLEGRN
CCHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAKTARTLFDKVWDAHVVVQREDGQSLLWIDRHFLHEGSFHAFDKVAGRGASLARPDLT
CCHHHHHHHHHHCCCEEEEEECCCCEEEEEEHHHHCCCCCHHHHHHHCCCCCCCCCCCE
FGVEDHYVPTRVRQLDLIDPAIRRMMEQLRANTLRHKVRLFDVDDPAQGIVHVVGPEQGL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCC
TLPGLSIVCGDSHTATHGAFGALAFGIGASEVAHVLMTQTLWQNRPKRLRIRVEGRLAPG
CCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
IVAKDVALSIIAAIGADGAAGHAIEYAGSVVRGLSMEGRLTLCNLSIEAGGRCGMIAPDA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCC
TTLDYVRGRPYAPEGALFDAAAEAWLALGSDADAEFDREVVLDGDAIAPIVTWGTSPEDA
CHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCC
LPITATVPDPERVDDAAKAAHIRDAVSYMDLRPGQRLSDIAVDRVFIGSCTNSRIEDLRA
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCCHHHHHHHH
AAAVLAGRSAKVPGLVSPGSALVKRQAEEEGLDAIFRAAGLEWVEAGCSMCVGMNGDLVP
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHEEECCCCCCCC
AGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDMRPLLEGRN
CCHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA