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The map label for this gene is fis [H]

Identifier: 158423728

GI number: 158423728

Start: 2420832

End: 2421377

Strand: Direct

Name: fis [H]

Synonym: AZC_2104

Alternate gene names: 158423728

Gene position: 2420832-2421377 (Clockwise)

Preceding gene: 158423727

Following gene: 158423730

Centisome position: 45.08

GC content: 65.75

Gene sequence:

>546_bases
ATGTCGATGACGGAGACCACCACACCCCGCCTGCTGATCGTCGAGGATGACGCGTCCTTCGCGCGCGCCCTGCGCCGCTC
CTTCGAACGGCGAGGCTACGAAGTCCGGCTGACCAGCAGTCTCGAAGGCGTGCAGTCCGTGCTCTCCGAAGTGACGCCGG
CCTATGCGGTCGTGGACCTGAAGCTCGCTGGCGGTTCCGGCCTTGAGTGCGTGAAGGAACTGCATGGCCGCGACGGTGAC
ATGCGCATCGTCGTCCTCACCGGTTTTGCCAGCATCGCGACCGCCGTGGAGGCCATCAAGCTCGGCGCCTGCCACTATCT
CGCCAAGCCGGCGAACACGGACGACATCGAGGCAGCCTTCGGCAAGGGGGGCGGCGACCCGACCATCGCGGTCACGGCCC
GTCCCACGTCGCTGAAAACGCTGGAGTGGGAACGCATCCACGAAACGCTGCTGGAAACCGACTTCAACATTTCCGAGACC
GCCCGCCGTCTCGGCATGCATCGCCGCACGCTCGCCCGCAAGCTCGAGAAGAAGCGTGTTCCCTGA

Upstream 100 bases:

>100_bases
GTCAATGTGGTCCGCAAGCTTGGAGGCACCGTAACAGCGCAGAACGAGGCTGACGGAGCGTCCGTCATCCTGGATTTGCC
GCTGCATGCTCTCTCACCCG

Downstream 100 bases:

>100_bases
GGTCGTCCTGGCCCGGCGGAGCCGTCAGGCGACGGCGGCGCCCGCTGCGGATCCGGCAAGGCGTCCCAGCACCACGGCGG
TGAGCAGGCCATTCCCTGAC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSMTETTTPRLLIVEDDASFARALRRSFERRGYEVRLTSSLEGVQSVLSEVTPAYAVVDLKLAGGSGLECVKELHGRDGD
MRIVVLTGFASIATAVEAIKLGACHYLAKPANTDDIEAAFGKGGGDPTIAVTARPTSLKTLEWERIHETLLETDFNISET
ARRLGMHRRTLARKLEKKRVP

Sequences:

>Translated_181_residues
MSMTETTTPRLLIVEDDASFARALRRSFERRGYEVRLTSSLEGVQSVLSEVTPAYAVVDLKLAGGSGLECVKELHGRDGD
MRIVVLTGFASIATAVEAIKLGACHYLAKPANTDDIEAAFGKGGGDPTIAVTARPTSLKTLEWERIHETLLETDFNISET
ARRLGMHRRTLARKLEKKRVP
>Mature_180_residues
SMTETTTPRLLIVEDDASFARALRRSFERRGYEVRLTSSLEGVQSVLSEVTPAYAVVDLKLAGGSGLECVKELHGRDGDM
RIVVLTGFASIATAVEAIKLGACHYLAKPANTDDIEAAFGKGGGDPTIAVTARPTSLKTLEWERIHETLLETDFNISETA
RRLGMHRRTLARKLEKKRVP

Specific function: Member of the two-component regulatory system regB/regA. Involved in transactivating anaerobic expression of the photosynthetic apparatus. It is a transcriptional regulator that is responsible for activating expression of the puf, puh, and puc operons in

COG id: COG4567

COG function: function code TK; Response regulator consisting of a CheY-like receiver domain and a Fis-type HTH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=26.1261261261261, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=113, Percent_Identity=30.0884955752212, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR002197
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 19842; Mature: 19711

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTETTTPRLLIVEDDASFARALRRSFERRGYEVRLTSSLEGVQSVLSEVTPAYAVVDL
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEEEE
KLAGGSGLECVKELHGRDGDMRIVVLTGFASIATAVEAIKLGACHYLAKPANTDDIEAAF
EECCCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
GKGGGDPTIAVTARPTSLKTLEWERIHETLLETDFNISETARRLGMHRRTLARKLEKKRV
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
P
C
>Mature Secondary Structure 
SMTETTTPRLLIVEDDASFARALRRSFERRGYEVRLTSSLEGVQSVLSEVTPAYAVVDL
CCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEEEE
KLAGGSGLECVKELHGRDGDMRIVVLTGFASIATAVEAIKLGACHYLAKPANTDDIEAAF
EECCCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
GKGGGDPTIAVTARPTSLKTLEWERIHETLLETDFNISETARRLGMHRRTLARKLEKKRV
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA