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The map label for this gene is dadB [H]

Identifier: 158423689

GI number: 158423689

Start: 2378407

End: 2379588

Strand: Direct

Name: dadB [H]

Synonym: AZC_2065

Alternate gene names: 158423689

Gene position: 2378407-2379588 (Clockwise)

Preceding gene: 158423688

Following gene: 158423690

Centisome position: 44.29

GC content: 67.77

Gene sequence:

>1182_bases
ATGCGCGGAAATGCGGACGATCCTGCCGTAAACTTTGCACTGCAAAACGGCGCGGATGATGCCATGAACGATTATCGGGC
GAAAACCATGCTTGCGGATGGCGGCTGTGGTGTCCTCACCATCGATCTCGGTGCCCTCGCGCGCAATTACCGCAAGCTGA
AAGAGACCGCCGCGCCTGCCGCCGTCGCTGCCGTGGTGAAGGCCGATGCCTACGGGCTTGGGGCTGGGCTCGTCAGCGAG
CGCCTCTATGCGGAAGGCTGCCGGCACTTCTTCGTCGCCCATTTCGTCGAGGCCGTGCGCCTGCGCCCGACCCTGCCGGT
GGAAGCGACCCTTCTCGTCCTTAACGGCCTGATGCCGGGCGAGGAGGAGCACGCGGCGGACCTGGGCGTGACGCCGGTCA
TCAATTGCCTTGAGCAGGCGGCCGCCTGGGCGACAGAGGCGAAGGTCCGTGGGACACGCCTCAAGGCCGCGATCCAGATT
GATACCGGCATGTCTCGCCTCGGTCTGTCGGAGGCGGATGTCGCGTCCTTGCGGGCGGACAGCCTGTTCGCCGAGGCGGT
CGAGCCCATCCTGCTTATGAGCCATCTCGCCTGTGCCGATACGCCGGCCGCTCCGGCGAGCGCCGGCCAGCTGGCCGTCA
TGCAGCGTGTTGCAGCACACTTCCCCGGCCTGCCGGTGTCGCTCGCCAATTCCGGCGGCGTCTTCCTTGGCGGCGCCTTC
CATTTCGCCCTCGCGCGGCCGGGCATTGCCGTTTATGGCGGCGCGCCCGTGAGCGACGTTGCCAATCCCATGGAGCCGGT
GGTCCGCATCGACATCCCGGTCATCCAGGCGCGAACCGTGCCGGCGGGCACGGCGGTGGGCTATGGCGGCACCTTCGTCA
CCACCGGCGAGACGCGGCTCGCGACGCTGAGCGGCGGCTATGCGGACGGCATTCCACGCCATCTCAGCTCCAGCGGCGCC
CTCTACTATCAGGGCGTGCGTTTGCCCATCGCCGGGCGCGTCTCCATGGACAGCCTCACTGTGGATGCCTCGGCGCTGCC
GGAAGGGGGCCTCCGGCTCGGCACCATGGTCGAATTCGTCGGCCCCAGCCAATCGCTGGAACAGGTGGCCGCCGACGCCG
GCACCATCTCCTACGAGATCCTGACCCGCCTCGGCCGTCGCTATCGCCGGGTCTACATCTGA

Upstream 100 bases:

>100_bases
CCGCGCAGGACGGACGGTCTCTCAAACGGCTTCGCGTTTCGTGCGTGGCCTCGAACGGAGTTGTGATATCAGCATTAAAA
TCCGCTGTTTCGTGCAGTAA

Downstream 100 bases:

>100_bases
CTATTCCAACAAGAACAAAATCCTACAGAGGGTGACATGCGGATCATCGTTCTGGGCGCCGGCGTCATCGGCGTCACCTC
CGCCTATTTCCTGGCGAAGG

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRGNADDPAVNFALQNGADDAMNDYRAKTMLADGGCGVLTIDLGALARNYRKLKETAAPAAVAAVVKADAYGLGAGLVSE
RLYAEGCRHFFVAHFVEAVRLRPTLPVEATLLVLNGLMPGEEEHAADLGVTPVINCLEQAAAWATEAKVRGTRLKAAIQI
DTGMSRLGLSEADVASLRADSLFAEAVEPILLMSHLACADTPAAPASAGQLAVMQRVAAHFPGLPVSLANSGGVFLGGAF
HFALARPGIAVYGGAPVSDVANPMEPVVRIDIPVIQARTVPAGTAVGYGGTFVTTGETRLATLSGGYADGIPRHLSSSGA
LYYQGVRLPIAGRVSMDSLTVDASALPEGGLRLGTMVEFVGPSQSLEQVAADAGTISYEILTRLGRRYRRVYI

Sequences:

>Translated_393_residues
MRGNADDPAVNFALQNGADDAMNDYRAKTMLADGGCGVLTIDLGALARNYRKLKETAAPAAVAAVVKADAYGLGAGLVSE
RLYAEGCRHFFVAHFVEAVRLRPTLPVEATLLVLNGLMPGEEEHAADLGVTPVINCLEQAAAWATEAKVRGTRLKAAIQI
DTGMSRLGLSEADVASLRADSLFAEAVEPILLMSHLACADTPAAPASAGQLAVMQRVAAHFPGLPVSLANSGGVFLGGAF
HFALARPGIAVYGGAPVSDVANPMEPVVRIDIPVIQARTVPAGTAVGYGGTFVTTGETRLATLSGGYADGIPRHLSSSGA
LYYQGVRLPIAGRVSMDSLTVDASALPEGGLRLGTMVEFVGPSQSLEQVAADAGTISYEILTRLGRRYRRVYI
>Mature_393_residues
MRGNADDPAVNFALQNGADDAMNDYRAKTMLADGGCGVLTIDLGALARNYRKLKETAAPAAVAAVVKADAYGLGAGLVSE
RLYAEGCRHFFVAHFVEAVRLRPTLPVEATLLVLNGLMPGEEEHAADLGVTPVINCLEQAAAWATEAKVRGTRLKAAIQI
DTGMSRLGLSEADVASLRADSLFAEAVEPILLMSHLACADTPAAPASAGQLAVMQRVAAHFPGLPVSLANSGGVFLGGAF
HFALARPGIAVYGGAPVSDVANPMEPVVRIDIPVIQARTVPAGTAVGYGGTFVTTGETRLATLSGGYADGIPRHLSSSGA
LYYQGVRLPIAGRVSMDSLTVDASALPEGGLRLGTMVEFVGPSQSLEQVAADAGTISYEILTRLGRRYRRVYI

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=363, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790487, Length=365, Percent_Identity=33.4246575342466, Blast_Score=147, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 40871; Mature: 40871

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGNADDPAVNFALQNGADDAMNDYRAKTMLADGGCGVLTIDLGALARNYRKLKETAAPA
CCCCCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCCEEEEEHHHHHHHHHHHHHHHCHH
AVAAVVKADAYGLGAGLVSERLYAEGCRHFFVAHFVEAVRLRPTLPVEATLLVLNGLMPG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
EEEHAADLGVTPVINCLEQAAAWATEAKVRGTRLKAAIQIDTGMSRLGLSEADVASLRAD
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCHHHHHHHHHH
SLFAEAVEPILLMSHLACADTPAAPASAGQLAVMQRVAAHFPGLPVSLANSGGVFLGGAF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHH
HFALARPGIAVYGGAPVSDVANPMEPVVRIDIPVIQARTVPAGTAVGYGGTFVTTGETRL
HHHHHCCCEEEECCCCHHHHCCCCCCEEEEECCEEEEECCCCCCEECCCCEEEECCCCEE
ATLSGGYADGIPRHLSSSGALYYQGVRLPIAGRVSMDSLTVDASALPEGGLRLGTMVEFV
EEECCCCCCCCHHHHCCCCCEEEECEEECEECCEECCCEEECHHCCCCCCCCHHHHHEEC
GPSQSLEQVAADAGTISYEILTRLGRRYRRVYI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGNADDPAVNFALQNGADDAMNDYRAKTMLADGGCGVLTIDLGALARNYRKLKETAAPA
CCCCCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCCEEEEEHHHHHHHHHHHHHHHCHH
AVAAVVKADAYGLGAGLVSERLYAEGCRHFFVAHFVEAVRLRPTLPVEATLLVLNGLMPG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
EEEHAADLGVTPVINCLEQAAAWATEAKVRGTRLKAAIQIDTGMSRLGLSEADVASLRAD
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCHHHHHHHHHH
SLFAEAVEPILLMSHLACADTPAAPASAGQLAVMQRVAAHFPGLPVSLANSGGVFLGGAF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHH
HFALARPGIAVYGGAPVSDVANPMEPVVRIDIPVIQARTVPAGTAVGYGGTFVTTGETRL
HHHHHCCCEEEECCCCHHHHCCCCCCEEEEECCEEEEECCCCCCEECCCCEEEECCCCEE
ATLSGGYADGIPRHLSSSGALYYQGVRLPIAGRVSMDSLTVDASALPEGGLRLGTMVEFV
EEECCCCCCCCHHHHCCCCCEEEECEEECEECCEECCCEEECHHCCCCCCCCHHHHHEEC
GPSQSLEQVAADAGTISYEILTRLGRRYRRVYI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]