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The map label for this gene is shiA [H]

Identifier: 158423572

GI number: 158423572

Start: 2248724

End: 2250226

Strand: Direct

Name: shiA [H]

Synonym: AZC_1948

Alternate gene names: 158423572

Gene position: 2248724-2250226 (Clockwise)

Preceding gene: 158423569

Following gene: 158423577

Centisome position: 41.88

GC content: 65.14

Gene sequence:

>1503_bases
ATGCAGACCATATCGAATATCGATTGGCGTGCCGCGGCGACTTCAACGAGAGTGCCCCGCAGGGCCGGCACAACGACCGG
CCAAGGTTCCGGTCACATCCGAAGATACTGGCCACGGCGCGCGCCGTGGTCTCGATGGATGCTGCCCGGTCCGACCCGAC
AATCACCAGAGGCGGACCCGATGAACGACCATGTCCCCGTGCGCGCCACCGCGGCCCGCGCGCCCCAGCCACGCAGCATG
CGCAAGATCGCCTTCGCGAGCGTCATCGGCACCACCGTCGAGTGGTACGATCTGTTCGTCTTCGGCACCGCATCGGCGCT
CGTCTTCAACAAGGTGTTCTTTCCGAGCTTCGATCCGCTCGCCGGCACGCTGCTGGCGTTCGGCACCTTCGCTTCGGCCT
ATCTTGCGCGCATCGTTGGTGCGGCGCTCTTCGGCCACTTCGGCGACAGGCTCGGCCGCAAGTCCATGCTGTTGACCTCT
TTGCTCATCATGGGGCTCGCCACCTTCGCCATAGGCCTCCTACCGAACTATTCGGTCATCGGCATTTGGGCTCCGATCCT
CCTTCTGACGCTCCGGCTCATTCAGGGCCTCGCGCTGGGCGGCGAATGGGGTGGTGCGGTGCTCATGGCGGTGGAACATG
CGCCGGACAACCGCCGGGGCCTCTATGGCTCCTGGGTGCAGATCGGCGTGCCCGCCGGCACGCTGCTCGCCAATTTCGCC
TTCCTCATCGCCAATGCGCTCATGTCCACGGAGGCGCTCGTCTCCTGGGGCTGGCGCATTCCATTCCTGGTCAGCATCCT
GCTGGTGGCGGTCGGGCTTTACGTGCGGCTCAACACGGCCGAGACGCCGTCCTTCCAGAAGGTGAAGACGGCGGGCGCGC
AAGCGAGCGTTCCTTTCGTCGCGCTGATGGCGAAGAACTGGAAGCGGGTGGTTCTGGGCGGTGTCGCCACCATGTCCACC
GGCACCTCCTTCAATCTCATCGTCGCCTTCGGCCTCACCTATGGCACGCAGACGCTGGGCTTCTCGCGCAATGAGATGCT
CTCCATCGCGCTCCTGTCCTGCGGTGCCTGCCTGCTGCTGCTTCCGGCCTTCGGCCTCCTCTCCGACGTGATCGGCCGCA
AGCCGGTGATCGTCGGCGGCATCATCGCGGAGGCCATCGTTGCCTTCCCGCTGTTCTGGCTCATGGACACCAAGGTCTTC
AGCCTCGCGCTGGTCGGCTATCTGCTGATGATGACGGCGTTCGCCGCCAATTACGGGCCGATCGCCACCTTCCTCGCGGA
GCTGTTCGGCACCAAGGTGCGTTATTCGGGCCTGTCCATCAGCTACATGCTCTCCGGCGTGCTGGGCAGCGCGGCGACGC
CCATCGTCACCACGGCACTGCTTCAGGCGACCGGCACGGGATCGTCGGTCGCCTGGTACATGATCGGCTCGGCTGCCATC
TCGACCATCGCGCTGCTGATGCTGGTGGAGACGCTGAAGGCCGATCTCGCCGAAGCGCACTGA

Upstream 100 bases:

>100_bases
GGACCTTGCTGCCTGTAAGGGAACGGCTGTGCCTGCCGGCCACGCCGCGATGACATCTGTTCTAGAGCGGCTCTGCGATG
GCGGAAAATAGAAACCGGCA

Downstream 100 bases:

>100_bases
CGTGCGGGCGGGAGCGGCCGGCTCCCGCGATCACTCACGGCCCGACCGGTGCCTCTATGTCGGCGTTCGCATCCTCGAAC
GCCGACATTTCCTCCATGAA

Product: permeases

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MQTISNIDWRAAATSTRVPRRAGTTTGQGSGHIRRYWPRRAPWSRWMLPGPTRQSPEADPMNDHVPVRATAARAPQPRSM
RKIAFASVIGTTVEWYDLFVFGTASALVFNKVFFPSFDPLAGTLLAFGTFASAYLARIVGAALFGHFGDRLGRKSMLLTS
LLIMGLATFAIGLLPNYSVIGIWAPILLLTLRLIQGLALGGEWGGAVLMAVEHAPDNRRGLYGSWVQIGVPAGTLLANFA
FLIANALMSTEALVSWGWRIPFLVSILLVAVGLYVRLNTAETPSFQKVKTAGAQASVPFVALMAKNWKRVVLGGVATMST
GTSFNLIVAFGLTYGTQTLGFSRNEMLSIALLSCGACLLLLPAFGLLSDVIGRKPVIVGGIIAEAIVAFPLFWLMDTKVF
SLALVGYLLMMTAFAANYGPIATFLAELFGTKVRYSGLSISYMLSGVLGSAATPIVTTALLQATGTGSSVAWYMIGSAAI
STIALLMLVETLKADLAEAH

Sequences:

>Translated_500_residues
MQTISNIDWRAAATSTRVPRRAGTTTGQGSGHIRRYWPRRAPWSRWMLPGPTRQSPEADPMNDHVPVRATAARAPQPRSM
RKIAFASVIGTTVEWYDLFVFGTASALVFNKVFFPSFDPLAGTLLAFGTFASAYLARIVGAALFGHFGDRLGRKSMLLTS
LLIMGLATFAIGLLPNYSVIGIWAPILLLTLRLIQGLALGGEWGGAVLMAVEHAPDNRRGLYGSWVQIGVPAGTLLANFA
FLIANALMSTEALVSWGWRIPFLVSILLVAVGLYVRLNTAETPSFQKVKTAGAQASVPFVALMAKNWKRVVLGGVATMST
GTSFNLIVAFGLTYGTQTLGFSRNEMLSIALLSCGACLLLLPAFGLLSDVIGRKPVIVGGIIAEAIVAFPLFWLMDTKVF
SLALVGYLLMMTAFAANYGPIATFLAELFGTKVRYSGLSISYMLSGVLGSAATPIVTTALLQATGTGSSVAWYMIGSAAI
STIALLMLVETLKADLAEAH
>Mature_500_residues
MQTISNIDWRAAATSTRVPRRAGTTTGQGSGHIRRYWPRRAPWSRWMLPGPTRQSPEADPMNDHVPVRATAARAPQPRSM
RKIAFASVIGTTVEWYDLFVFGTASALVFNKVFFPSFDPLAGTLLAFGTFASAYLARIVGAALFGHFGDRLGRKSMLLTS
LLIMGLATFAIGLLPNYSVIGIWAPILLLTLRLIQGLALGGEWGGAVLMAVEHAPDNRRGLYGSWVQIGVPAGTLLANFA
FLIANALMSTEALVSWGWRIPFLVSILLVAVGLYVRLNTAETPSFQKVKTAGAQASVPFVALMAKNWKRVVLGGVATMST
GTSFNLIVAFGLTYGTQTLGFSRNEMLSIALLSCGACLLLLPAFGLLSDVIGRKPVIVGGIIAEAIVAFPLFWLMDTKVF
SLALVGYLLMMTAFAANYGPIATFLAELFGTKVRYSGLSISYMLSGVLGSAATPIVTTALLQATGTGSSVAWYMIGSAAI
STIALLMLVETLKADLAEAH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=38.2775119617225, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=41.7840375586854, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=32.2274881516588, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=30.5210918114144, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082231, Length=402, Percent_Identity=21.6417910447761, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082404, Length=363, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 53368; Mature: 53368

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTISNIDWRAAATSTRVPRRAGTTTGQGSGHIRRYWPRRAPWSRWMLPGPTRQSPEADP
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
MNDHVPVRATAARAPQPRSMRKIAFASVIGTTVEWYDLFVFGTASALVFNKVFFPSFDPL
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AGTLLAFGTFASAYLARIVGAALFGHFGDRLGRKSMLLTSLLIMGLATFAIGLLPNYSVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIWAPILLLTLRLIQGLALGGEWGGAVLMAVEHAPDNRRGLYGSWVQIGVPAGTLLANFA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHH
FLIANALMSTEALVSWGWRIPFLVSILLVAVGLYVRLNTAETPSFQKVKTAGAQASVPFV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCCHH
ALMAKNWKRVVLGGVATMSTGTSFNLIVAFGLTYGTQTLGFSRNEMLSIALLSCGACLLL
HHHHCCCHHHHHCCHHEECCCCCEEEEEEEEHHHCCHHCCCCHHHHHHHHHHHHHHHHHH
LPAFGLLSDVIGRKPVIVGGIIAEAIVAFPLFWLMDTKVFSLALVGYLLMMTAFAANYGP
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IATFLAELFGTKVRYSGLSISYMLSGVLGSAATPIVTTALLQATGTGSSVAWYMIGSAAI
HHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHH
STIALLMLVETLKADLAEAH
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTISNIDWRAAATSTRVPRRAGTTTGQGSGHIRRYWPRRAPWSRWMLPGPTRQSPEADP
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
MNDHVPVRATAARAPQPRSMRKIAFASVIGTTVEWYDLFVFGTASALVFNKVFFPSFDPL
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AGTLLAFGTFASAYLARIVGAALFGHFGDRLGRKSMLLTSLLIMGLATFAIGLLPNYSVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIWAPILLLTLRLIQGLALGGEWGGAVLMAVEHAPDNRRGLYGSWVQIGVPAGTLLANFA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHH
FLIANALMSTEALVSWGWRIPFLVSILLVAVGLYVRLNTAETPSFQKVKTAGAQASVPFV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCCHH
ALMAKNWKRVVLGGVATMSTGTSFNLIVAFGLTYGTQTLGFSRNEMLSIALLSCGACLLL
HHHHCCCHHHHHCCHHEECCCCCEEEEEEEEHHHCCHHCCCCHHHHHHHHHHHHHHHHHH
LPAFGLLSDVIGRKPVIVGGIIAEAIVAFPLFWLMDTKVFSLALVGYLLMMTAFAANYGP
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IATFLAELFGTKVRYSGLSISYMLSGVLGSAATPIVTTALLQATGTGSSVAWYMIGSAAI
HHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHH
STIALLMLVETLKADLAEAH
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]