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The map label for this gene is trmH [H]

Identifier: 158423491

GI number: 158423491

Start: 2137628

End: 2138497

Strand: Direct

Name: trmH [H]

Synonym: AZC_1867

Alternate gene names: 158423491

Gene position: 2137628-2138497 (Clockwise)

Preceding gene: 158423490

Following gene: 158423498

Centisome position: 39.81

GC content: 68.05

Gene sequence:

>870_bases
ATGCCCGGCGCCGGAACCGACAGCACGAAGCCCTGGATCGCCGGCGGGCCGGTGGTGATCCTCGTCGAGCCACAGCTCGG
CGAGAATGTCGGCTCGGCGGCGCGGGCCATGGGCAATTTCGGCCTCTCCCGTCTGCGCGTGGTGAACCCGCGCGAGGGCT
GGCCGAACGAGAAGGCGCGCATCTTCTGCGCCGGTGCGGACCGAATTCTCGACGACGCCCAGCTTTTCCCCGACCTGCGC
AGCGCGCTGGCGGACGTGTCCTATGCCGTGGCCACCACGGCCCGCGAGCGCGGCATGGCCAAGCCGGTGAATGGCGCGGA
CGTGGTCGCGCAGGATGCGGTGGTGCGCCTGAAGGCGGGTGAGGACGTGGCGCTGGTCTTCGGCCGCGAGCGCACGGGTC
TCTATACGGAAGAAGTGTCGCTCTGCGATTCGATCCTCACCCTGCCGGTCAATCCCGCTTTCGCCTCGCTGAATCTGGCT
ACCGCCGTGGCTGTGACCGCCTATGAATGGTCGAAGGCGGCCACCGGCAATGCTCTGCCCTTCGTCATGCCCGAGCGCTC
GCCGCCGGCGGACAGGGTCGATCTTTTCGCCTTCTTCGATCATCTGGAGCGGGAACTGGAGGAGGCGGCTTTTTTCCGTT
CGCCGGAAAAGGCGCCTTCGACCACGCGCAACATCCGCAACATCTTCCATCGCTGCGAGCTCACCAAGCAGGATCTCGCC
ACCTTGCACGGCATGATCACCGCGCTGGTTGAGGGGCGCGAAGGCCGTGAGCAGCGCAAGGCCGAGCGCAACGCCAAGGC
CGCCGCCAAGCGCAATCCGGCCTCCGGCACGGACATCGGCGCAGCCGGGGAGGGCGGCGCGGAGGGTTGA

Upstream 100 bases:

>100_bases
GCTCCATCCGGTTCCGGCTCTATGACGGGCGCATGTCTGAGCACGTTCTTTCTTCCATGCCGCCCGCTGTCCCTTACCCG
TCCTGCCGGGGAGGGCGCTG

Downstream 100 bases:

>100_bases
GCCCGACGCGGGTCTGCCATCCCCACATCCTCGCTGGGCTGGAAACCTTTGCCGGGAAACGGTTTGGCGGTCCAGTCCGC
CGGGCGCCATCCACAGGTGG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPGAGTDSTKPWIAGGPVVILVEPQLGENVGSAARAMGNFGLSRLRVVNPREGWPNEKARIFCAGADRILDDAQLFPDLR
SALADVSYAVATTARERGMAKPVNGADVVAQDAVVRLKAGEDVALVFGRERTGLYTEEVSLCDSILTLPVNPAFASLNLA
TAVAVTAYEWSKAATGNALPFVMPERSPPADRVDLFAFFDHLERELEEAAFFRSPEKAPSTTRNIRNIFHRCELTKQDLA
TLHGMITALVEGREGREQRKAERNAKAAAKRNPASGTDIGAAGEGGAEG

Sequences:

>Translated_289_residues
MPGAGTDSTKPWIAGGPVVILVEPQLGENVGSAARAMGNFGLSRLRVVNPREGWPNEKARIFCAGADRILDDAQLFPDLR
SALADVSYAVATTARERGMAKPVNGADVVAQDAVVRLKAGEDVALVFGRERTGLYTEEVSLCDSILTLPVNPAFASLNLA
TAVAVTAYEWSKAATGNALPFVMPERSPPADRVDLFAFFDHLERELEEAAFFRSPEKAPSTTRNIRNIFHRCELTKQDLA
TLHGMITALVEGREGREQRKAERNAKAAAKRNPASGTDIGAAGEGGAEG
>Mature_288_residues
PGAGTDSTKPWIAGGPVVILVEPQLGENVGSAARAMGNFGLSRLRVVNPREGWPNEKARIFCAGADRILDDAQLFPDLRS
ALADVSYAVATTARERGMAKPVNGADVVAQDAVVRLKAGEDVALVFGRERTGLYTEEVSLCDSILTLPVNPAFASLNLAT
AVAVTAYEWSKAATGNALPFVMPERSPPADRVDLFAFFDHLERELEEAAFFRSPEKAPSTTRNIRNIFHRCELTKQDLAT
LHGMITALVEGREGREQRKAERNAKAAAKRNPASGTDIGAAGEGGAEG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=36.3636363636364, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1790865, Length=155, Percent_Identity=38.7096774193548, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30886; Mature: 30755

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGAGTDSTKPWIAGGPVVILVEPQLGENVGSAARAMGNFGLSRLRVVNPREGWPNEKAR
CCCCCCCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCE
IFCAGADRILDDAQLFPDLRSALADVSYAVATTARERGMAKPVNGADVVAQDAVVRLKAG
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEECC
EDVALVFGRERTGLYTEEVSLCDSILTLPVNPAFASLNLATAVAVTAYEWSKAATGNALP
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FVMPERSPPADRVDLFAFFDHLERELEEAAFFRSPEKAPSTTRNIRNIFHRCELTKQDLA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHH
TLHGMITALVEGREGREQRKAERNAKAAAKRNPASGTDIGAAGEGGAEG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGAGTDSTKPWIAGGPVVILVEPQLGENVGSAARAMGNFGLSRLRVVNPREGWPNEKAR
CCCCCCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCE
IFCAGADRILDDAQLFPDLRSALADVSYAVATTARERGMAKPVNGADVVAQDAVVRLKAG
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEECC
EDVALVFGRERTGLYTEEVSLCDSILTLPVNPAFASLNLATAVAVTAYEWSKAATGNALP
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FVMPERSPPADRVDLFAFFDHLERELEEAAFFRSPEKAPSTTRNIRNIFHRCELTKQDLA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHH
TLHGMITALVEGREGREQRKAERNAKAAAKRNPASGTDIGAAGEGGAEG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA